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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Mechanisms of antibiotic action and their relationship to the shape of the inhibition zones in E-test
 Brzobohatá, Hana ; Fišer, Radovan (advisor) ; Vrana, Branislav (referee)
The aim of this work is antibiotic susceptibility testing, especially the method of Etest. Etest is a widespread technique due to its simplicity and accuracy in both research and clinical conditions. Understanding the method and its influencing factors is necessary for accurate reading of the MIC (minimum inhibitory concentration) and understanding of bacteria- antibiotic interactions. The purpose of the study was to demonstrate connections between antibiotic action mechanism and zone of inhibition shape. Also, to point out the obstacles that might complicate results interpretation. The pictures of Etest results from research papers were compared to the models of antibiotic diffusion. The zone of inhibition shape is affected by the diffusion rate, molecular characteristics of antibiotic, by the bacterial strain performances and by the specific bacteria-antibiotic interactions.
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Complexity of analytical sampling procedures for persistent toxic compounds. Water Sampling by Semipermeable membrane device and Data Analysis.
 Ocelka, Tomáš ; Ševčík, Jiří (advisor) ; Vrána, Branislav (referee) ; Punčochář, Miroslav (referee)
Charles University Faculty of Natural Sciences Department of Analytical Chemistry Summary of Doctoral Thesis STUDY AND DEVELOPMENT OF SAMPLING METHODS FOR PERSISTENT ORGANIC COMPOUNDS METHODOLOGY OF DATA ANALYSIS FOR POPS ASSESSMENT Dipl. Ing. Tomas Ocelka Prague 2010 Complexity of analytical sampling procedures of toxic compounds. Water Sampling by Semipermeable membrane device and Data analysis. Summary of Doctoral Thesis. TABLE OF CONTENTS 3 TABLE OF CONTENTS Section Page TABLE OF CONTENTS .....................................................................................3 1 INTRODUCTION/THEORY ..................................................................5 2 EXPERIMENTAL PART..........................................................................7 A. Semipermeable membrane device (SPMDs).................................7 B. Used biota, bioassays.....................................................................8 C. Sampling protocol ..........................................................................8 D. Analytical methods.........................................................................9 E. Statistical and gnostic methods .....................................................9 F. Applications.................................................................................. 11 3...
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