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Management of a contributory organization
VOŠAHLÍKOVÁ, Marie
The aim of this diploma thesis was to develop a proposal for improved use of funds in a contributory school facility. The work consists of two parts, theoretical and practical. The theoretical part tries to briefly explain the topics related to the management of the contributory organization and the basic concepts used in financial analysis, non-profit sector and school facilities. The practical part is focused on the analysis of the finances of the contributory organization and the financial analysis was used. Sources of funding, school expenditures and financial results are mapped for the period 2016-2020. At the end of the work is an evaluation and recommendations for improved financial management.
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Modulatory effect of monovalent ions on δ-opioid receptors
Vošahlíková, Miroslava ; Svoboda, Petr (advisor) ; Jakubík, Jan (referee) ; Kršiak, Miloslav (referee)
The exact role of opioid receptors in drug addiction and modulatory mechanism of action of monovalent cations on these receptors are still not fully understood. Our results support the view that the mechanism of addiction to morphine is primarily based on desensitization of μ- and δ-opioid receptors. Desenzitization of agonist response proceeds already at the level of G protein functional activity. Long-term exposure of rats to morphine resulted in increase of number of δ-opioid receptors and change of their sensitivity to sodium ions. Analysis of the effect of different monovalent ions on agonist binding in δ-OR- Gi1α (Cys351 -Ile351 )-HEK293 cell line confirmed the preferential sensitivity of δ-opioid receptor to sodium ions. We have distinguished the high- and low-affinity Na+ sites. Biophysical analysis of interaction of lithium, sodium, potassium and cesium ions with plasma membranes isolated from HEK293 cells with the help of fluorescent probes indicated that monovalent ions interact, in low-affinity manner, with the polar, membrane-water interface of membrane bilayer. Key words: morphine, forebrain cortex, opioid receptors, G proteins, monovalent ions, plasma membrane, fluorescence spectroscopy.
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Modulatory effect of monovalent ions on δ-opioid receptors
Vošahlíková, Miroslava ; Svoboda, Petr (advisor) ; Jakubík, Jan (referee) ; Kršiak, Miloslav (referee)
The exact role of opioid receptors in drug addiction and modulatory mechanism of action of monovalent cations on these receptors are still not fully understood. Our results support the view that the mechanism of addiction to morphine is primarily based on desensitization of μ- and δ-opioid receptors. Desenzitization of agonist response proceeds already at the level of G protein functional activity. Long-term exposure of rats to morphine resulted in increase of number of δ-opioid receptors and change of their sensitivity to sodium ions. Analysis of the effect of different monovalent ions on agonist binding in δ-OR- Gi1α (Cys351 -Ile351 )-HEK293 cell line confirmed the preferential sensitivity of δ-opioid receptor to sodium ions. We have distinguished the high- and low-affinity Na+ sites. Biophysical analysis of interaction of lithium, sodium, potassium and cesium ions with plasma membranes isolated from HEK293 cells with the help of fluorescent probes indicated that monovalent ions interact, in low-affinity manner, with the polar, membrane-water interface of membrane bilayer. Key words: morphine, forebrain cortex, opioid receptors, G proteins, monovalent ions, plasma membrane, fluorescence spectroscopy.
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Journey of a Hero: The Treatment of Mythology in the Work of William Butler Yeats
Vošahlíková, Martina ; Ženíšek, Jakub (advisor) ; Grmela, Josef (referee)
The thesis deals primarily with the influence of mythology in the work of William Butler Yeats and particularly focuses on his depiction of the ancient Celtic warrior Cuchulainn. The work briefly introduces the background of Irish mythology and its recordings; however the main interest lies in the interpretation of the myth by the Irish literary movement represented by W.B. Yeats and its contribution to the constitution of Ireland's national identity. The literary portrayal is contrasted with the way the hero Cuchualinn was recalled by the political leaders of the Irish revolution to illustrate the process of national symbols' formation. The challenge of the resulting establishment exemplified by the work of Mary T. Condren in the final part of the thesis points out to the opportunity of intentional selection of such symbols and opens the discussion of art's authority and influence.
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