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Molecular biological analyses of Sand gudgeon (Romanogobio kesslerii) in the waters of the Czech Republic and Slovakia
Mendel, Jan ; Lusk, Stanislav ; Lusková, Věra ; Koščo, J. ; Papoušek, Ivo ; Halačka, Karel ; Vetešník, Lukáš
Kessler's gudgeon belongs to critically endangered species protected by both national and European legislation. We present the first findings concerning the genetic diversity of 5 R. kesslerii populations from the Czech and Slovak rivers. On the basis of sequencing of the control region, as well as of the first intron of the S7 r-protein, 30 specimens were analysed. Altogether nine haplotypes were identified. Analyses of mtDNA showed the existence of two groups differing by 6.2%. The first group included the specimens from the rivers Bečva and Ipel and the second group included the specimens from the rivers Topl’á, Laborec and Morava. The inter-population diversity was 0.4% within the first group and it ranged from 0.5% to 1.0% within the second group. The combination of the methods of sequencing of parts of mtDNA and nDNA and allozyme analysis revealed the presence of hybrids of G. gobio and R. kesslerii in the Morava and Ipel’ rivers.
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On the occurrence of rare and protected lamprey and fish species in the Morava River drainage area
Lusk, Stanislav ; Lusková, Věra ; Lojkásek, B. ; Halačka, Karel ; Vetešník, Lukáš ; Merta, L.
The occurrence of a considerable number of rare and protected lamprey and fish species in the Czech Republic is exclusive for the drainage area of the Morava River. Eudontomyzon mariae occurs only in the Račí potok Brook. Lampetra planeri occurs in a section ca 20 km long in the upper part of the Morava R. Romanogobio kessleri initially occurred only in the Bečva R. After 2000 was newly recorded in the Morava R. Romanogobio albipinnatus occurs in the lower reaches of the rivers Morava and Dyje; locally, we have observed in the upper parts of these rivers. After 1990 species Pelecus cultratus, Gymnocephalus schraetser, G. baloni, Zingel streber, and Z. zingel re-occurred in the confluence of Morava and Dyje rivers. The only actual range of Sabanejewia balcanica in the Czech Republic is in a short section of the Vlára R. (the Váh R. drainage area) near the boundary of Slovakia. Cobitis elongatoides occurs, in its pure form, only in the upper part of the Dyje R. drainage area.
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Several biological characteristic of Carassius auratus
Vetešník, Lukáš ; Lusk, Stanislav ; Spurný, P.
Based on realized research it was found that the present population of Carassius auratus occurred in the lower reach of the river Dyje does not have any significant differences in plastic and meristic characters when compared to the individuals occupying this area 27 years ago. The growth depends on the living conditions of studied individuals. The growth of 2n males was significantly slower comparing to 3n females.
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Diploid, triploid and tetraploid silver crucian carp Carassius auratus from the lower reach of Dyje River: the first results of reproductive characteristics and experimental hybridization
Flajšhans, Martin ; Lusková, Věra ; Vetešník, Lukáš ; Halačka, Karel ; Rodina, Marek ; Lusk, Stanislav ; Gela, David
Diploid, triploid and tetraploid males of silver crucian carp, Carassius auratus, produced live motile spermatozoa, capable of fertilization. Flow cytometry for the relative DNA content in male gametes revealed spermatozoa of diploid males to be haploid, those of triploid males to be aneuploid and those of tetraploid male to be aneuploid and/or diploid. Concentration of spermatozoa and the percentage of live spermatozoa showed a tendency to drop with increasing ploidy level and this effect was significant for the tetraploid male compared to those of lower ploidy levels.
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Some biological characteristics of the invasion species Perccottus glenii
Halačka, Karel ; Vetešník, Lukáš ; Koščo, J.
The occurrence of this exotic species was first observed in the Latorica River, E Slovakia, in 1998. Perccottus glenii occupied the rivers Latorica, Bodrog and Tisza together with the aquatic habitats in their floodplains. We carried out analysis of growth, haematology, fecundity, morphology of skin and gonad of individuals Amur sleeper in the locality at Svata Maria (the Bodrog River drainage area). In the locality under study the invasion of Perccottus glenii resulted in an almost total destruction of numerous populations of the native species Umbra krameri, Carassius carassius, and Leucaspius delineatus. In the past, these species were among the most numerous in that locality. Perccottus glenii has appeared to be a typical 'invasive' species that is a serious threat for native fish species showing identical habitat requirements.
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The ploidy of Carassius auratus in the Czech Republic in light of the artificial reproduction
Halačka, Karel ; Vetešník, Lukáš ; Flajšhans, M.
State of population of Carassius "gibelio" (the sex ratio and ploidy level) was for a long time observed in the Dyje river floodplain, where it has expanded by natural migration from the Danube river through Morava river in 1975-1976. Results show the time dynamics for both parameters, with increasing proportion of males and increasing proportion of diploids of both sex and the formation diploid-polyploid complex. Diploid, triploid and tetraploid males and females of silver crucian carp, Carassius auratus, as well as males of silver Blicca bjoerkna and Rutilus rutilus, were used in mating. The results show different reaction of females on used sperm even in the same group of ploidy. The existence of some groups of the individuals with a different strategy of reproduction in frame single population could be the explanation.
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