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Analysis of Andrographis leaves extract
Tonarová, Marta ; Jahodář, Luděk (advisor) ; Macáková, Kateřina (referee)
1 CHARLES UNIVERSITY IN PRAGUE FACULTY OF PHARMACY IN HRADEC KRÁLOVÉ DEPARTMENT OF PHARMACEUTICAL BOTANY AND ECOLOGY Analysis of Andrographis leaves extract. Marta Tonarová, 2010 Abstract: The analysis of the extract of leaves Andrographis paniculata (Acanthaceae) was accomplished. The commercial ethanol extract with the contents of andrographolides not higher than 10 % represented the tested material. In case of using the standard andrographolide, the thin layer chromatography has proved its very low amount in the extract only, in spite of the fact that studying literature consideres its contents as majority. The identity of the standard was verified by mesuring its NMR spectrums, which refers to the informations in the studying literature. Contents of andrographolides was fixed by the high performance liquid chromatography extanded to the standard, with using of isocratic elution and spectrophotometric detection by the wave lenght 223 nm. Total contents of the andrographolides in the extract was 12,23 % and consequently it refers to the production protocol. The main component in the contents of the extract was isolated by using preparative TLC. On the basis of fixing the NMR spectrums and their confrontation with the studying literature it was determined as 14-deoxy-11,12-didehydroandrographolide.
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Biologically active metabolites of plants VIII. Alkaloids of Fumaria officinalis L. and their biological activity
Mazel Tonarová, Marta ; Cahlíková, Lucie (advisor) ; Opletal, Lubomír (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical botany Candidate Mgr. Marta Mazel Tonarová Consultant Prof. Ing. Lucie Cahlíková, Ph.D. Title of Thesis Biologically active metabolites of plants VIII. Alkaloids of Fumaria officinalis L. and their biological activity Mazel Tonarová M.: Biologically active metabolites of plants VIII. Alkaloids of Fumaria officinalis L. and their biological activity. Charles University, Faculty of Pharmacy in Hradec Králové. Department of Pharmaceutical Botany, Hradec Králové, 2021. Rigorosum thesis. Eleven fractions were prepared for further processing, this was based on previously performed column chromatography of diethyl ether extract of Fumaria officinalis L. Two alkaloids were isolated by using preparative TLC of fraction 138-178, followed by crystallization. The alkaloids were identified based on their structural analysis - using MS and MNR spectrum, specific optical rotation and melting point as (-)-fumaritine and (+)-parfumine. Both alkaloids were tested for their inhibitory activities against prolyloligopeptidase, acetylcholine- and butyrylcholinesterases. The measured IC50 values were compared with the known standard values. Neither (-)-fumaritine nor (+)-parfumine showed more significant inhibitory activities...
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Analysis of Andrographis leaves extract
Tonarová, Marta ; Jahodář, Luděk (advisor) ; Macáková, Kateřina (referee)
1 CHARLES UNIVERSITY IN PRAGUE FACULTY OF PHARMACY IN HRADEC KRÁLOVÉ DEPARTMENT OF PHARMACEUTICAL BOTANY AND ECOLOGY Analysis of Andrographis leaves extract. Marta Tonarová, 2010 Abstract: The analysis of the extract of leaves Andrographis paniculata (Acanthaceae) was accomplished. The commercial ethanol extract with the contents of andrographolides not higher than 10 % represented the tested material. In case of using the standard andrographolide, the thin layer chromatography has proved its very low amount in the extract only, in spite of the fact that studying literature consideres its contents as majority. The identity of the standard was verified by mesuring its NMR spectrums, which refers to the informations in the studying literature. Contents of andrographolides was fixed by the high performance liquid chromatography extanded to the standard, with using of isocratic elution and spectrophotometric detection by the wave lenght 223 nm. Total contents of the andrographolides in the extract was 12,23 % and consequently it refers to the production protocol. The main component in the contents of the extract was isolated by using preparative TLC. On the basis of fixing the NMR spectrums and their confrontation with the studying literature it was determined as 14-deoxy-11,12-didehydroandrographolide.
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