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National Repository of Grey Literature

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	Antidiuretic hormone Tenorová, Dana ; Netopilová, Miloslava (advisor) ; Šimůnek, Tomáš (referee) Detailed record
	Evaluation of DNA damage and repair in various pathological stages Tenorová, Dana ; Němečková, Ivana (advisor) ; Rathouská, Jana (referee) author: Dana Tenorová title: Evaluation of DNA damage and repair in various pathological stages Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové field of study: Specialist in Laboratory Methods Chronic obstructive pulmonary disease (COPD) is a serious disease characterized by airflow obstruction that is caused by chronic bronchitis or pulmonary emphysema. As a result of these changes occurs in the organism the oxidative stress with subsequent damage to various biomolecules, which can be measured using a wide range of methods. The primary aim of our study was the evaluation of DNA damage in patients with COPD and comparison to the damage found in the healthy control group. We used electrophoretic method comet assay and we analyzed various types of DNA damage - DNA single strand breaks (SSB), oxidized purines and oxidized pirimidines. We used two forms of this method, i.e. a classical alkaline comet assay and its enzymatic modification, where we worked with endonuclease III (Endo III) and formamidopyrimidin-DNA-glycosylase-III (FPG). Our results showed the significantly higher levels of DNA damage in patients with COPD compared with the control group, especially when using the modified comet assay. In conclusion, we have examined the possibility of comet assay... Detailed record
	Antidiuretic hormone Tenorová, Dana ; Šimůnek, Tomáš (referee) ; Netopilová, Miloslava (advisor) Detailed record

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