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Application of analytical methods for analysis of dyes employed in works of art.
Svobodová, Eva ; Bosáková, Zuzana (advisor) ; Kopecká, Ivana (referee) ; Feltl, Ladislav (referee)
Natural organic dyes suffer from degradation during the time. Therefore, the works of art need to be restored and the identification of dyes by analytical methods is important here. The possibilities of using three separation (thin-layer chromatography - TLC, capillary zone electrophoresis - CZE, and micellar electrokinetic chromatography - MEKC) and two spectroscopic (infrared microspectroscopy - IR and Raman microspectroscopy) methods for the identification of red dyes (acaroid, brazil, dragon blood, kamala, logwood, cochineal, madder, lac, and sandalwood) were studied and critically discussed. In TLC, under optimised conditions (silicagel with octadecyl, 80/20 (v/v) MeOH/1% TEA in 0.1 mol·dm-3 acetate buffer, pH 5, and 80/20 (v/v) MeOH/1% TEA in 0.025 mol·dm-3 tetraborate buffer, pH 7 and 9), the stain broadening of sandalwood, logwood, acaroid, dragon blood, and brazil was observed. Carminic acid, haematein and purpurin were not detected. A comparison of CZE and MEKC showed that MEKC was superior as it permits separation of all analytes, using 0.015 mol·dm-3 sodium dodecyl sulfate in 0.01 mol·dm-3 tetraborate buffer, pH 8.5, at a voltage of 20 kV. The developed method was validated and applied to the identification of dyes in Rubia tinctorum, Rubia cordifolia, and in the mycelium of fungi...
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Nestinars in southeastern part of Bulgaria
Svobodová, Eva ; Šalanda, Bohuslav (advisor) ; Štěpánková, Jana (referee)
Diploma thesis Nestinars in southeastern part of Bulgaria focuses on the material and spiritual culture of Nestinars, a small group of people living in region of Strandja. The body of work describes in detail the specific phenomena of spiritual culture, which cut across the structure of traditional and contemporary Bulgarian folk customs. It describes ceremonies that are part of the clan, family, annual festivals and church calendar. Nestinars as a subculture, whose socio-cultural negotiations and ideas to some extent differ from the majority of the Orthodox population, they are perceived externally as part of it. In this work we meet with traditional clothing, folklore music and holy sacrifice. The parallel with the Greek rite anastnaria is mentioned in the work as well. Both groups represent a traditional ceremony where believers show their devotion to the saints by dance and walking on burning wood charcoal. Keywords nestinars - social group tradition - the transmission of customs and beliefs from generation to generation usually in framwork of certain culture or social group ceremony - a formal religious or public occassion, typically one celebraing particular event or anniversary; or ritual observances or procedures performed at grand and formal occassions to keep group's norms and values of...
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Constitution of interested persons in physical activity and their aims in reconditioning center of VŠTJ MEDICINA PRAHA
Svobodová, Eva ; Matoulek, Martin (advisor) ; Svačina, Štěpán (referee)
Title: Constitution of interested persons in physical aktivity and their aims in reconditioning center of VŠTJ MEDICINA PRAHA Objectives: The first aim of this work is to find out, what is the main motivation of the patiens with the metabolic syndrome in the beginning of the physical excercise. The second aim is through analysis of clients of reconditioning center for assuring sufficient professionally led classes of physical aktivity. Methods: Socio-epidemiologic research on sample of clients of reconditioning center of VŠTJ MEDICINA PRAHA at first class. For research questionnaires were used. Results: The survey involved 84 new clients, 69 women and 15 men whose average age was 48.6 ± 13.8 years. The largest group, 64%, consisted of respondents with secondary education. As the main motivation of 62 respondents reported weight loss. In the comparison with the current population is in the file higher incidence of diabetes and hypertension. Keywords: metabolic syndrome, obesity, hypertension, diabetes, motivation
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Analysis of pigments from integument of Graphosoma semipunctatum.
Krajíček, Jan ; Svobodová, Eva (referee) ; Bosáková, Zuzana (advisor)
Pterines belong to an important group of compounds, acting as pigments in many species. Some of them are probably responsible for characteristic coloration of the insect group (Hereroptera). This coloration is considered to be a visual warning signal for optically orientating predators (birds, lizards etc.). In this work, pterines in the species of Graphosoma semipunctatum have been investigated by high performance liquid chromatography. To develope an appropriate separation method, the reverse-phase separation mode with C18 stationary phase (Spherisorb ODS 2) and binary mobile phase (organic modifier/buffer or water) was used. The effect of type and content of organic modifiers (methanol, ethanol, tetrahydrofuran) and concentration of phosphate buffer pH 3.0 (10 - 30 mM) in the mobile phases on retention and separation behavior of the studied pterines (leukopterin, biopterin, xanthopterin, isoxanthopterin and erythropterin) was studied. Under the optimized separation conditions (5/95 (v/v) methanol/20 mM phosphate buffer, pH 3.0, flow rate 0.7 ml.min-1 , UV detection at 290 nm), the extract from the integument of Graphosoma semipunctatum was analyzed.
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Social work with pregnant drug addicted women
Svobodová, Eva ; Velemínský, Miloš (advisor) ; Šípek, Jiří (referee) ; Miovský, Michal (referee)
Myšlenka věnovat se profesně tématu tak citlivému, stigmatizovanému a přitom tak reálnému jako je problematika těhotných drogově závislých žen vznikala pozvolna během mé profesní činnosti orientované na drogově závislou populaci. Poté, co jsem v Českých Budějovicích začala realizovat Terénní program pro drogově závislé (2000) a současně vybudovala Rodičovskou skupinu pro rodiče (2000), jejichž děti mají problémy s drogami jsem měla možnost se v rámci mého studijního pobytu ve Spojených Státech (2002) seznámit s celou řadou projektů orientovaných na pomoc těhotným drogově závislým ženám a matkám v různých fázích vývoje závislosti. Tato problematika mne zcela zaujala.
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The emotional intentions in the interwar political cartoons (1918-1939)
Svobodová, Eva ; Köpplová, Barbara (referee) ; Sekera, Martin (advisor)
Diploma thesis "The Emotional Intentions in the Interwar Political Cartoons (1918-1939)" deals with the view of selected events and personalities, by which is expected a highly emotional (she had: emotionally heady - laden) public evaluation. This view of political themes is followed in the period October 1918 - December 1919 and May 1938 - March 1939, in the objectively politically dynamic periods and within the field of collective emotions hypertrophic periods. The basic thesis states: Caricatures exude mainly negative emotions (though at the same time - secondarily - may involve in the perception positive emotions too, the overall tone of the caricatures and their persuasive intention is primarily an evaluative negation of the shown). Aim of my thesis is to try - by using semiotic analysis - to identify what emotions could be excited by recipients and to provide the emotional agenda. 1 also mention stereotypes in the representation of individual phenomena, but their representation is not the core part of the work. The working method is an innovation of traditional approaches to the interpretation of the cartoons, their forms and contents. Its aim is to verify productivity of this chosen method for any other use. The primary sources are copies of satirical magazines and newspapers, and secondary...
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