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Advanced retinal vessel segmentation methods in colour fundus images
Svoboda, Ondřej ; Jan, Jiří (referee) ; Odstrčilík, Jan (advisor)
Segmentation of vasculature tree is an important step of the process of image processing. There are many methods of automatic blood vessel segmentation. These methods are based on matched filters, pattern recognition or image classification. Use of automatic retinal image processing greatly simplifies and accelerates retinal images diagnosis. The aim of the automatic image segmentation algorithms is thresholding. This work primarily deals with retinal image thresholding. We discuss a few works using local and global image thresholding and supervised image classification to segmentation of blood tree from retinal images. Subsequently is to set of results from two different methods used image classification and discuss effectiveness of the vessel segmentation. Use image classification instead of global thresholding changed statistics of first method on healthy part of HRF. Sensitivity and accuracy decreased to 62,32 %, respectively 94,99 %. Specificity increased to 95,75 %. Second method achieved sensitivity 69.24 %, specificity 98.86% and 95.29 % accuracy. Combining the results of both methods achieved sensitivity up to72.48%, specificity to 98.59% and the accuracy to 95.75%. This confirmed the assumption that the classifier will achieve better results. At the same time, was shown that extend the feature vector combining the results from both methods have increased sensitivity, specificity and accuracy.
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Single railway electrification system design
Svoboda, Ondřej ; Radil, Lukáš (referee) ; Pelc, Jiří (referee) ; Drápela, Jiří (advisor)
The main topic of the thesis is the design of single-track electrification of a direct current system of 3 kV and an alternating current system of 5 kV 50 Hz and then economically evaluate it over a period of 30 years. The work deals with basic traction systems and also deals with the calculation of main parameters of electric trains. The thesis describes in detail the procedure for the design of the electrification of the track and a partial introduction to the simulation programs that were used for energy calculations.
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Study of cardiomyocytes viability using confocal microscopy
Chutný, Pavel ; Svoboda, Ondřej (referee) ; Čmiel, Vratislav (advisor)
This work describes the study of cardiomyocytes viability using confocal microscopy. The theoretic introduction covers the basic principle of fluorescence together with fluoresceins in cells biology. The struction of cardiomyocytes, their selection from the lively heart and the methods of viability examination is comprised in the next part. The third topic is about confocal mikroskopy and its posibilities in cardiomyocytes imaging. In next chapters are described the experiments and their evaluating.
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Plasmide DNA isolation from bacteria and transfection to HEK293 cell line
Bačovská, Kristýna ; Chmelíková, Larisa (referee) ; Svoboda, Ondřej (advisor)
Plasmid DNA is commonly used in fields of molecular biology and genetic engineering. This work deals with methods of DNA isolation and topics related to this procedure. In the experimental part of the work, phenol-chloroform extraction is used. First of all, plasmids Channelrhodopsin-2, ASAP1 and Kir 2.1 were amplified in bacterial strain DH5. 22 isolations were accomplished and the yield was validated using gel electrophoresis and transfection to HEK293 cell line. The most successful isolation was the isolation of plasmid ASAP1, the overall percentage success rate was 30 %.
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Web Information System Design Proposal
Svoboda, Ondřej ; Koutný, Miroslav (referee) ; Dydowicz, Petr (advisor)
This bachelor’s thesis deals with evaluation and design of a website, where will also be designed information website system (further IS). It will have a form of a calendar and it should help the firm to better time planning, organization of work, guidance of employees and echo checking of information. In IS there will be modules for evidence of workers, customers, orders, services, costs and particular jobs. IS design will be like that so there is an opportunity to add extending modules.
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Friction and air feeders for paper sheets
Svoboda, Ondřej ; Košťál, David (referee) ; Brandejs, Jan (advisor)
The thesis is focused on summarizing the evidence on the issue of paper sheets feeding at the final processing of documents. The thesis includes dividing of paper feeders, their characterization, and description of friction and air feeders, which are most widespread. As a model for the description methods of sheets feeding were used machinery CFM 700 and GPM 540A of Cyklos company. The thesis also compares friction and air feeding from the constructional point of view. The conclusion focuses on the new methods in the paper feeding in future.
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Electrophysiological characterization of Kir2.1 membrane channel
Měsíčková, Klára ; Chmelíková, Larisa (referee) ; Svoboda, Ondřej (advisor)
The topic of this thesis is electrophysiological characterization of Kir2.1 membrane channel. Inward rectifier potassium channel Kir2.1 is located in muscular, heart and nerve cells and its dysfunction causes various diseases. Practical part of this stage is focused on cultivation of the HEK293T cell line that is used to transfection of the plasmid Kir2.1 and subsequent measurement of the ionic current through the electrophysiological method patch-clamp in whole-cell mode.
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Analysis of ZED stereocamera in outdoor environment
Svoboda, Ondřej ; Věchet, Stanislav (referee) ; Krejsa, Jiří (advisor)
The Master thesis is focused on analyzing stereo camera ZED in the outdoor environment. There is compared ZEDfu visual odometry with commonly used methods like GPS or wheel odometry. Moreover, the thesis includes analyses of SLAM in the changeable outdoor environment, too. The simultaneous mapping and localization in RTAB-Map were processed separately with SIFT and BRISK descriptors. The aim of this master thesis is to analyze the behaviour ZED camera in the outdoor environment for future implementation in mobile robotics.
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Information System for a School
Svoboda, Ondřej ; Fiala, Jiří (referee) ; Částek, Petr (advisor)
This bachelor's thesis describes designing and creation of information system for Higher school of education, which will improve communication between students and lectors. Final application is written in PHP scripting language and uses MySQL database and AJAX technology. During implementation object-oriented programming paradigm was used.
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