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Patterning of excitable cells on multi-electrode arrays
Slavík, Jan ; doc. RNDr. Petr. Skládal, CSc. (referee) ; Průcha,, Jaroslav (referee) ; Hubálek, Jaromír (advisor)
Práce se zabývá zarovnáváním excitabilních buněk na multielektrodových polích. Nejprve bylo analyzováno zarovnávání excitabilních buněk. Byly použity embryonální neurony z hippocampusu potkanů a HL-1 buňky, které jsou odvozeny z AT-1 linie nádorových myších atriálních kardiomyocytů. Zarovnávání bylo testováno na drážkovaných površích a na površích s materiály s různou buněčnou afinitou. Bylo prokázáno, že na drážkových površích se ve směru drážek zarovnávají neurony i HL-1 buňky, ale na površích s různou chemickou affinitou se zarovnávají pouze neurony. Dále byly vyrobeny vlastní multielektrodová pole, na těchto multielektrodových polích byly kultivovány HL-1 buňky a byl změřen a analyzován akčních potenciál HL-1 buněk. Cílem bylo prokázat, že je možné měřit akční potenciál na vyrobených multielektrodových polích. Pro zarovnání buněk na multielektrodovém poli bylo vyrobeno speciální multieletrodové pole s uniformním povrchem. Toto multielektrodové pole je nazýváno planární multielektrodové pole. Planární multielektrodové pole bylo vyrobeno speciálním vyrobním procesem. Vrstvy planárního multielektrodového pole byly deponovány na pomocný substrát v opačném pořadí. Pomocný substrátem pro depozici byla křemíková deska, na který byla nadeponována další pomocná vrstva zlata. Horní izolační vrstva planárního multielektrodové pole byla deponována jako první a nejspodnější vrstva substrátu byla nadeponována jako poslední. Planární multielektrodové pole i s pomocnou zlatou vrstvou bylo strhnuto s křemíku díky nízké adhezi zlata ke křemíku a planární multielektrodové pole se otočilo vzhůru nohama. Pomocná zlatá vrstva byla odstraněna mokrým leptadlem a tím bylo planární multielektrodové pole dokončeno. Na planárním multielektrodovém poli byly zarovnány HL-1 buňky do pruhů chemickou metodou pomocí kombinace otisku adhezní látky a následným potažení neotisklých ploch anti-adhezní látkou. Elektrofyziologické vlastnosti zarovnaných HL-1 buněk byly změřeny pomocí planárního multielektrodového pole. Tímto experimentem byla představena výrobní technologie pro výrobu planárních multielektrodových polí a toto planární multielektrodové pole bylo úspěšně testováno pro zarovnání HL-1 buněk na jeho povrchu kombinací otisku adhezní látky a potahování antiadhezivním činidlem.
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Design of the CSR Concept in the Company Management System
Macháčová, Zuzana ; Slavík, Jan (referee) ; Putnová, Anna (advisor)
The diploma thesis deals with the design of the CSR concept in a selected company. On the basis of theoretical knowledge together with a suitably chosen methodology, the main part of the work follows, where the level of corporate social responsibility in the economic, social and environmental areas is evaluated. The results are followed by a design part, which contains ideas and measures leading to the creation of the company's concept.
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ID Gallery
Slavík, Jiří ; Sochorová,, Zuzana (referee) ; Struhala, Karel (advisor)
The aim of the thesis is to design a two-storey gallery and café building in Chrudim. The thesis has three parts. The first part presents the architectural and structural designs for the building permit. The building consists of two parts: a two -storey gallery and a one-storey café with necessary facilities. There is a green roof above the gallery and a terrace above the café. The envelope wall is insulated with mineral wool and loadbearing structures are designed from reinforced concrete. Internal non-load-bearing structures are designed of aerated concrete and plasterboard. The floors are monolithic reinforced concrete. I evaluated the building for Energy Performance Certificate. The second part proposes the design of selected building services: HVAC, artificial lighting and rainwater management system. There are two mechanical ventilation unit with heat recovery, which partly provide heating and cooling. The remaining heat is provided by local district heating grid. The designs also include concept of a photovoltaic plant on the roof. Third part assesses the life cycle assessment (LCA) of the structures. All the proposed compositions have the same main function - to ensure the stability of the building, fire safety, weather protection and thermal comfort. Six similar compositions of the vertical wall structure are proposed, combining different types of load-bearing masonry with ETICS system, ventilated facade, variant of aerated concrete blocks or VELOX system formwork. For the selected compositions, the functional unit is 1 m2 with a service life of 50 years and a heat transfer coefficient of U=0.216 ± 0.002 W/m2.K.
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Proposal of the Company's Financial Plan
Bednarovičová, Andrea ; Slavík, Jan (referee) ; Žižlavský, Ondřej (advisor)
This master’s thesis deals about topic of financial planning of the company. At first there are defined theoretical ways out of financial planning. The next part analyses contemporary state of the company. Subsequently there is worked out the financial plan for the years 2012-2014 in in realistic, optimistic and pessimistic variant. The end of the thesis forms evaluation of created financial plans.
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Assessment of the Impact of Planning on Land Prices
Slavík, Jan ; Šestáková, Romana (referee) ; Doležalová, Monika (advisor)
This diploma thesis describes methodology background for price evaluation of real properties. The case study presents the evaluation of selected land in cadastral area Podolí u Brna, town Podolí in different conditions of town and country planning. The administrative price and market price are obtained from evaluation in three different steps of country planning. The obtained results are interpreted and changes in prices are outlined with an effect of town and country planning changes.
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Proposal of Marketing Mix of a Selected Company
Kubíncová, Ema ; Slávik, Jozef (referee) ; Milichovský, František (advisor)
This diploma thesis deals with a company, that sells gastronomy supplies on the Slovak market. It focuses on the proposal of a marketing mix of a new ecological disposable food and drink packaging that the company intends to import from the Netherlands. The work is divided into three main parts. The first part is theoretical and explains the basic theoretical knowledge needed to understand the topic. The second part of the thesis is analytical and includes an analysis of the current marketing mix of the company and its marketing environment. The analytical part also includes a marketing survey, the aim of which is to find out the preferences of the target segment. The last part of the work presents proposals and recommendations in the field of marketing mix, formulated based on the results obtained through analyses.
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Life cycle assessment of municipal waste
Vlach, Josef ; Slavík, Jan (referee) ; Jícha, Jaroslav (advisor)
The aim of this study is to present methodology life cycle (LCA) and Life Cycle Assessment of disposal the municipal solid waste chain. There is described the procedure of generation of the LCA studies according to the standards ČSN EN ISO 14 040 and ČSN EN ISO 14 044 in the first and second part. In the third part of this diploma there are described LCA analyses that have already been created. The software GEMIS (Global Emission Model for Integrated System) is described in the fourth part. The computer model GEMIS has been used as a calculation tool in this study. The fifth part of this study includes waste management and different kinds of disposal municipal solid waste. In this part is describe basic data about landfilling and incieration. There is shown LCA analysis of disposal municipial solid waste in the sixth part. Data processing was carried out by means of the software GEMIS 4.3. The results were interpreted for incineration and landfilling. Finally there is mentioned results for 1 TJ different elektrical energize to.
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