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Investment Construction Co-financed from EU Funds
Sklenářová, Hana ; Mach, Drahomír (referee) ; Hrabincová, Dagmar (advisor)
This theses is focused on the European Union and EU funds. Description of structural policy and its history, with a focus on structural and cohesion funds. Furthermore, attention is focused on the subsidy received from the ROP southeast. In summary is given a new programming framework 2014-2020. The practical part of the thesis was to describe, analyze and evaluate the project co-financed by EU structural funds. The project was implemented within the ROP SE and tackle integrated transport in the region Breclav.
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Evaluation of phenolic substances content in plant material
Hollá, Marcela ; Sklenářová, Hana (advisor) ; Hroboňová, Katarína (referee) ; Štěrbová, Petra (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Marcela Hollá Supervisor: assoc. prof. PharmDr. Hana Sklenářová, Ph.D. Consultant: prof. RNDr. Dalibor Šatínský, Ph.D. Title of Doctoral Thesis: Evaluation of phenolic substances content in plant material Scientific community has always been interested in the research of natural substances. The main reasons are a huge diversity of substances themselves and their significant impact on human health. The development of new analytical and extraction methods, including new approaches for sample preparation, enable obtaining more detailed knowledge about the structure and biological properties of plant substances. A significant group consists of substances with antioxidant effects, such as phenolic compounds, which are an important part of human food. The presented dissertation thesis deals with determination of selected groups of natural substances from the point of view of specific requirements for chromatographic separation, choice of detection technique with regard to the selectivity and sensitivity of the determination, as well as the possibility of applying modern and green extraction approaches. The first part of the presented work is devoted to the issue of determination of selected plant...
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Aplication of separation methods for determination of biologically active substances
Bílková, Aneta ; Sklenářová, Hana (advisor) ; Česla, Petr (referee) ; Solich, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: RNDr. Aneta Bílková Supervisor: assoc. prof. PharmDr. Hana Sklenářová, Ph.D. Consultant: assoc. prof. PharmDr. Petr Chocholouš, Ph.D. Title: Application of separation methods for determination of biologically active substances The submitted dissertation thesis presents an annotated set of three publications, focusing on the application of separation methods for the determination of biologically active phenolic substances, pesticide residues in fruits, with regard to the use of modern storage technologies, and the application result - a utility model based again on monitoring the phenolic substances content with respect to different conditions of plant material drying. In the theoretical part, the target analytes are characterized from an analytical point of view, with respect to their physico-chemical properties, stability, antioxidantion activity. It also describes sample preparation methods for the determination of biologically active phenolic substances, selected extraction procedures and an overview of the methods used for the determination of the described substances in plant material. The main part of the dissertation deals with the development, optimization and validation of a rapid...
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Automated monitoring of Metridia luciferase released from genetically modified cell line
Jozičová, Natali ; Sklenářová, Hana (advisor) ; Horstkotte, Burkhard (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of: Analytical Chemistry Candidate: Natali Jozičová Supervisor: doc. PharmDr. Hana Sklenářová, Ph.D. Consultant: prof. Manuel Miró Title of the diploma thesis: Automated monitoring of Metridia luciferase released from genetically modified cell line. The diploma thesis reports automatic real-time monitoring of the release of Metridia luciferase from genetically modified cells. A previously optimized method for monitoring the natural release of luciferase from cells was used during the experiment. At the same time, the toxic effect of bile acids (chenodeoxycholic and deoxycholic acid) on changes in cell membrane permeability was tested. Liver epithelial cell line modified with the Metridia luciferase plasmid were used during the experiment. After this genetic modification, the cells were able to synthesize the active Metridia luciferase enzyme. This enzyme generates luminescence upon reaction with substrate coelenterazine. The emitted light was detected in the flow cell of a spectrofluorometer at a wavelength of 485 nm. The measurement was taken in a sequential injection system connected to a 3D printed module containing an insert with the tested cell monolayer. This automated system allowed real-time monitoring of the luciferase...
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Automated monitoring of sulisobenzone liberation through model membrane
Marel, David ; Sklenářová, Hana (advisor) ; Chocholouš, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: David Marel Supervisor: assoc. prof. PharmDr. Hana Sklenářová, Ph.D. Title of the diploma thesis: Automated monitoring of sulisobenzone liberation through model membrane This diploma thesis deals with the monitoring of biologically active substance liberation through a model membrane using sequential injection analysis. A UV - VIS spectrophotometer was used for the detection. The tested biologically active substance was sulisobenzone (one of the UV filters used in sunscreens) and the permeation through the polycarbonate membrane was monitored using a Franz cell. At the beginning of the work, a calibration curve was created in two media - water and phosphate buffer of pH 7.4. According to these curves, the concentrations - which were further used in the liberation tests - were selected. The given concentrations of sulisobenzone were prepared in different solvents, namely in water, in 10% ethanol, in 10% propylene glycol and in 5% propylene glycol. The tested solvents were selected based on the assumption of increasing the permeability of the membrane for the liberation of substances with limited solubility in the liberation medium. Each tested solution was measured for 2 hours, with individual...
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Development and validation of UHPLC method for determination of meloxicam in dissolution medium
Švaňová, Petra ; Matysová, Ludmila (advisor) ; Sklenářová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Petra Švaňová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Development and validation of a UHPLC method for determination of meloxicam in a dissolution medium This diploma thesis covers the development and subsequent validation of a UHPLC method for determination of meloxicam in a dissolution medium. The columm LiChrospher®, 5 µm RP-18 100 Å (125x4mm) was used for method development. As mobile phase were used methanol, isopropyl alcohol and phosphate buffer (50 mmol/l) in a 51:9:40 ratio, while the aqueous phase pH was adjusted to value 6.8. Flow rate was set to 1.0 ml/min, column temperature was 30řC and injection volume was set to 20 µl. Analytes were detected on the wavelength of 363 nm. The results of the partial validation showed that it is necessary to further optimise the method in order to provide correct results even in the dissolution medium after dissolution.
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Development of UHPLC-DAD method for separation of phenolic substances in plant material and extraction optimization
Turčanová, Anna ; Sklenářová, Hana (advisor) ; Šatínský, Dalibor (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Anna Turčanová Supervisor: doc. PharmDr. Hana Sklenářová, Ph.D. Advisor: Mgr. Marcela Hollá Title of the diploma thesis: Development of UHPLC-DAD method for separation of phenolic substances in plant material and extraction optimization This diploma thesis deals with development of UHPLC-DAD method for separation of selected phenolic substances (arbutin, chlorogenic, neochlorogenic, cryptochlorogenic, isochlorogenic A and caffeic acid, cynarin, catechin, epicatechin, rutin, hyperoside, hirsutrin, reynoutrin, guaiaverin, quercitrin and quercetin). UHPLC analysis of these phenolic substances took place on a Luna® Omega Polar C18 100 (150 x 2.1 mm; 1.6 μm) column with fully porous particles. As a part of the optimization method, a column with core-shell particles (Kinetex® Polar C18) and a column with a hybrid stationary phase (YMC-Triart C18 ExRS) were also tested. The sample injection volume was 2 μl, the flow rate has been set to 0.3 ml/min and temperature of the column has been set to 30 řC. As a mobile phase ultrapure water acidified with acetic acid to pH 2.8 in combination with acetonitrile has been used. The total analysis time was 17.5 min and detection was performed using a DAD detector at...
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The use of HPLC technique in the analysis of phenolic compounds in plant material of fruit trees
Adamcová, Anežka ; Šatínský, Dalibor (advisor) ; Sklenářová, Hana (referee) ; Vávra, Radek (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Anežka Adamcová Supervisor: Prof. RNDr. Dalibor Šatínský, Ph.D. Title of the Dissertation Thesis: The use of HPLC technique in the analysis of phenolic compounds in plant material of fruit trees Although fruits are well studied and known for their high content of antioxidants and positive health benefits, the main aim of the presented dissertation thesis consists in the characterization of biological active compounds in individual parts of fruit tree material (leaves, bark, buds, flowers, and wood chips). The theoretical part is focused on fruit trees and the characterization of phenolic compounds. Their properties and the effects based on current scientific studies are shown. A large part of thesis is dealing with the chromatographic methods for the separation of phenolic substances including the current trends. The extraction process is also included. The practical part deals with the validation and development of screening HPLC-DAD method for the analysis of monitored phenolic compounds (phloridzin, phloretin, chlorogenic acid, quercitrin, rutin, arbutin, 1,5-dicafeoylquinic acid, and 3,5-dicafeoylquinic acid) in individual parts of fruit trees. The main objective of this thesis is to...
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The use of HPLC technique in the analysis of phenolic compounds in plant material of fruit trees
Adamcová, Anežka ; Šatínský, Dalibor (advisor) ; Sklenářová, Hana (referee) ; Vávra, Radek (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Anežka Adamcová Supervisor: Prof. RNDr. Dalibor Šatínský, Ph.D. Title of the Dissertation Thesis: The use of HPLC technique in the analysis of phenolic compounds in plant material of fruit trees Although fruits are well studied and known for their high content of antioxidants and positive health benefits, the main aim of the presented dissertation thesis consists in the characterization of biological active compounds in individual parts of fruit tree material (leaves, bark, buds, flowers, and wood chips). The theoretical part is focused on fruit trees and the characterization of phenolic compounds. Their properties and the effects based on current scientific studies are shown. A large part of thesis is dealing with the chromatographic methods for the separation of phenolic substances including the current trends. The extraction process is also included. The practical part deals with the validation and development of screening HPLC-DAD method for the analysis of monitored phenolic compounds (phloridzin, phloretin, chlorogenic acid, quercitrin, rutin, arbutin, 1,5-dicafeoylquinic acid, and 3,5-dicafeoylquinic acid) in individual parts of fruit trees. The main objective of this thesis is to...
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