|
|
Cappillary electrophoresis of peptides and proteins
Sedláková, Pavla
This thesis is devoted to the development of new methods for the separation of peptides and proteins. Attention is focused on proteins that stick to the capillary wall (various dynamic coatings of the inner surface of the capillary). In this work many modes of the capillary electrophoreses or its combination with the mass spectrometry are used. The theoretical part describes CE separation mechanisms of peptides and proteins, problems of choose of right separation conditions and possibilities of suppressing adsorption of these compounds to the inner wall of the capillary. Furthermore it characterizes the individual modes of the capillary electrophoreses and their use for the separation of peptides and proteins, various types of detection and possibilities of improving of the detection sensitivity. The experimental part describes chemicals, instrumentation, materials and methods used for this study and the preparation of the individual samples is also described. Results and findings are summarized and discussed in the last part of this study. The individual parts of this work are devoted to these new developed methods for the separation of peptides and proteins: (i) Capillary electrophoresis of peptides and proteins with plug of Pluronic F 127 gel. (ii) Separation of poly(amidoamine) (PAMAM)...
|
|
|
Marketing of the luxury brand Dior
Sedláková, Pavla ; Procházková, Markéta (advisor) ; Horáková, Iva (referee)
The main topic of this bachelor thesis is the marketing of the luxury fashion brand Dior. The aim is to describe and analyze marketing methods. This thesis describes which tools Dior use to gain customers and how these tools work. The theoretical part of my work is focused on introducing marketing, marketing mix and marketing strategy. I was also devoted to luxury. The methodological part introduces selected methods of bachelor thesis and hypotheses. On of the part of the thesis is an analysis of questionnaire survey on the perception of the Dior brand and the term luxury. The practical part also includes interviews with fashion bloggers and their look at luxury, luxury brands and the Dior brand.
|
|
|
Cappillary electrophoresis of peptides and proteins
Sedláková, Pavla
This thesis is devoted to the development of new methods for the separation of peptides and proteins. Attention is focused on proteins that stick to the capillary wall (various dynamic coatings of the inner surface of the capillary). In this work many modes of the capillary electrophoreses or its combination with the mass spectrometry are used. The theoretical part describes CE separation mechanisms of peptides and proteins, problems of choose of right separation conditions and possibilities of suppressing adsorption of these compounds to the inner wall of the capillary. Furthermore it characterizes the individual modes of the capillary electrophoreses and their use for the separation of peptides and proteins, various types of detection and possibilities of improving of the detection sensitivity. The experimental part describes chemicals, instrumentation, materials and methods used for this study and the preparation of the individual samples is also described. Results and findings are summarized and discussed in the last part of this study. The individual parts of this work are devoted to these new developed methods for the separation of peptides and proteins: (i) Capillary electrophoresis of peptides and proteins with plug of Pluronic F 127 gel. (ii) Separation of poly(amidoamine) (PAMAM)...
|
|
|
ANALYSIS OF NON-ENZYMATIC POSTTRANSLATIONAL MODIFICATED (GLYCATED) ALBUMIN BY NANO-LC/MS/MS
Šťastná, Zdeňka ; Pataridis, Statis ; Sedláková, Pavla ; Mikšík, Ivan
Posttranslational modifications of proteins are important reactions, which significantly affect the function of proteins in the organism. In principle, they can be divided into enzymatic and non-enzymatic modifications. Non-enzymatic reactions include glycation (earlier called nonezymatic glycosylation), which plays an important role in the development of chronic complications of diabetes mellitus, uremia, in the process of aging and degeneration of the brain.This work deals with the study of glycated albumins (human serum albumin and bovine serum albumin). Methodologically we used nano-liquid chromatography coupled to Q-TOF mass spectrometer. In vitro modified proteins were cleavaged by trypsin and arising peptides were separated on C18 nano column with trap-column. Peptides and their modifications were analysed by high-resolution Q-TOF mass spectrometer MaXis with precision determination of mass below 2 ppm. We found some modifications of proteins. Besides well known carboxymethyllysine new ones were determined - create mass shift 78, 132 and 218. Origin of these modifications is discussed and possible structure is presented. All found modifications were allocated to the structure of proteins and reactivity to various oxo-compounds was also examined
|
|
|
Open-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested proteins
Mikšík, Ivan ; Lacinová, Kateřina ; Zmatlíková, Zdeňka ; Sedláková, Pavla ; Král, V. ; Sýkora, D. ; Řezanka, P. ; Kašička, Václav
Open-tubular capillary electrochromatography (OT-CEC) using bare gold nanoparticles-based stationary phase has been applied to separation of tryptic peptide fragments of native and glycated proteins, bovine serum albumin and human transferrin. OT-CEC with bare gold nanoparticles stationary phase was found to be a suitable technique for separation of complex peptide mixtures originating from enzymatic (tryptic) digestion of native and glycated bovine serum albumin and human transferrin, and for glycation studies of these proteins.
|
guest ::
RSS feed.