National Repository of Grey Literature 45 records found  previous11 - 20nextend  jump to record: Search took 0.00 seconds. 
Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice
Krejcarová, Jana ; Rathouská, Jana (advisor) ; Nachtigal, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice Author: Jana Krejcarová Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: This thesis was focused on the detection and subsequent evaluation of the expression of adhesion molecules ICAM-1, VCAM-1 and COX-2 and P-selectin in mouse aortas. Control and monitored group of mice differed in the genetic material, where monitored group of mice had unfunctional genes for apolipoprotein E and LDL receptor (apoE−/− /LDLR−/− ). Methods: For the study, two months old male mice of the C57BL/6J strain were used. Males were divided into two groups - control and monitored with unfunctional receptor for low- density lipoprotein and for apolipoprotein E. Both groups were fed by a standard diet for rodents. For detection and subsequent quantification of the expression of the markers VCAM-1, ICAM-1, COX-2 and P-selectin, Western blot analysis was used. Levels of soluble ICAM-1 and P-selectin in plasma were assessed by Luminex®. Results: Western blot analysis showed no significant difference in the expression of VCAM-1, ICAM-1, COX-2 and P-selectin between both groups of mice. Using the...
The recommended vaccination of the adult population in the Czech Republic
Nová, Petra ; Rathouská, Jana (advisor) ; Janďourek, Ondřej (referee)
Author: Petra Nová, DiS. Title: The recommended vaccination of the adult population in the Czech Republic Bachelor thesis Charles University, Faculty of Pharmacy in Hradec Králové Field of study: Medical Laboratory Technician Background: The aim is to refer the possibilities of recommended vaccines for adults and focus on the currently available vaccines on the Czech market. The partial aim of the work is to describe the importance of vaccination as such and partly to disprove the opinions of its uselessness. At the end, the work deals with data processing and evaluation of vaccines dispensing in two selected pharmacies. Methods: Statistical evaluation of vaccines dispensing in two private pharmacies in Jicin in 2014 - 2016. Evaluation includes the types of dispensed vaccines, gender and the age categories for which the individual vaccines are determined. Results: Concerning the dispensed vaccines, there were mainly vaccines against influenza, tick-borne encephalitis and viral hepatitis A. The most commonly prescribed vaccines were against the flu. In 2015, September-December period was recorded the largest number of these dispensed vaccines. Conversely, in 2016 there was the lowest interest in this vaccination. Persons between 45 - 60 years were the most represented age category for the flu...
Western blot analysis of selected oxidative stress markers in aorta of apoE/LDLR deficient mice
Čermáková, Martina ; Rathouská, Jana (advisor) ; Blažíčková, Kateřina (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Western blot analysis of selected oxidative stress markers in aorta of apoE/LDLR deficient mice Author of diploma thesis: Bc. Martina Čermáková Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: The aim of this diploma thesis was to evaluate expression of selected oxidative stress molecules (SOD-3, iNOS, catalase, HO-1) in cardiac aorta and nitrate and nitrite levels in plasma in two groups of mice. These two groups of mice varied in genetic features - the monitored group was, compared to control group of mice, deficient for apolipoprotein E and LDL receptor. Methods: For the study, 2 months old male mice were fed a standard rodent diet. The males were divided into two groups - control (C57BL/6J) and monitored (apoE-/- /LDLR- /- ). Western blot method was used to evaluate and quantify iNOS, SOD-3, catalase and HO-1 molecules in the aorta. HPLC method was used to quantify nitrate and nitrite levels in plasma. Result: HPLC method did not show statistically significant differences in the nitrate and nitrite levels in plasma between the two groups of examined mice. Western blot analysis of aortic samples showed statistically significantly higher SOD-3 expression (208.39 %) and a significantly...
Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice
Krejcarová, Jana ; Rathouská, Jana (advisor) ; Nachtigal, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice Author: Jana Krejcarová Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: This thesis was focused on the detection and subsequent evaluation of the expression of adhesion molecules ICAM-1, VCAM-1 and COX-2 and P-selectin in mouse aortas. Control and monitored group of mice differed in the genetic material, where monitored group of mice had unfunctional genes for apolipoprotein E and LDL receptor (apoE−/− /LDLR−/− ). Methods: For the study, two months old male mice of the C57BL/6J strain were used. Males were divided into two groups - control and monitored with unfunctional receptor for low- density lipoprotein and for apolipoprotein E. Both groups were fed by a standard diet for rodents. For detection and subsequent quantification of the expression of the markers VCAM-1, ICAM-1, COX-2 and P-selectin, Western blot analysis was used. Levels of soluble ICAM-1 and P-selectin in plasma were assessed by Luminex®. Results: Western blot analysis showed no significant difference in the expression of VCAM-1, ICAM-1, COX-2 and P-selectin between both groups of mice. Using the...
Up-to-date antiretroviral therapy
Teichmann, Radek ; Jílek, Petr (advisor) ; Rathouská, Jana (referee)
2 Abstract Radek Teichmann Modern antiretroviral therapy Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Health bioanalytics - Medical Laboratory Modern antiretroviral therapy is a form of treatment that suppresses the spread of the virus in the cell and in the case of some drugs prevents viral entry into the cell. The aim of this therapy is to restore the number of white blood cells to the original values. Combined antiretroviral therapy can effectively reduce HIV replication and slow down the progression of immunodeficiency. It will ensure a significant increase in life expectancy to HIV-infected patients. The Thesis discusses the treatment of diseases associated with HIV. The main objective is to create a comprehensive overview of the evaluation of drugs called antiretrovirals.
Multiple sclerosis - laboratory aspects
Lhotáková, Vendula ; Jílek, Petr (advisor) ; Rathouská, Jana (referee)
56 22 Abstract Author: Vendula Lhotáková Title: Multiple sclerosis - laboratory aspects Bachelor work Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové Field of study: Medical laboratory technician, combined study form Background: Bachler work thesis is written as a literary review. The main effort is to point to the diagnosis of multiple sclerosis, to set out its main risk factors highlighted immunological background and last but not least, to find possible biomarkers of disease. Main findings: Multiple Sclerosis (MS) is an inflammatory auto-immune disease affecting especially the younger generation. When the disease causes damage to the myelin -protective layer of axons (neuronal projections), it is characterized by inflammatory lesions in the brain causing CNS dysfunction. MS history dates back to the 14th century , although the reversal in the diagnosis of the disease occurred only in the 20s. Diagnosis is difficult because symptoms of the disease are not uniform. With technological advances, however, the diagnosis is considerably improved. Nowadays, diagnostic processes are based on a particular variety of laboratory tests including Magnetic resonance imaging. Very useful is the test of the cerebro spinal fluid, as well as other markers of the disease. RS is determined genetically and...
Immunohistochemical detection of PECAM-1, endoglin and VCAM-1 in aorta of transgenic mice with high levels of soluble endoglin
Brlicová, Monika ; Rathouská, Jana (advisor) ; Filipský, Tomáš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Immunohistochemical detection of PECAM-1, endoglin and VCAM-1 in aorta of transgenic mice with high levels of soluble endoglin Rigorous thesis Mgr. Monika Brlicová Consultant of rigorous thesis: PharmDr. Jana Rathouská, Ph.D. Background: We observed an effect of high level of human soluble endoglin (sEng) on an expression of tissue endoglin and adhesion molecules VCAM-1 and PECAM-1 in descendent aortas of experimental animals. Methods: 6 months old female transgenic mice of strain CBAxC5B7BL/6J with high plasma levels of human soluble endoglin (Sol-Eng+ ) and the same types of mice, which show signs of low plasma levels of human soluble endoglin as control mice were used for this rigorous thesis. Both groups were fed by high fat diet for 3 months. After that the levels of total cholesterol in the blood of these animals were biochemically determined and then statistically evaluated. The immunohistochemical method ImmPRESSTM was used for detection of protein expression in aortic sections. Results: Biochemical analysis showed a statistically insignificant difference in total cholesterol levels in both groups. Immunohistochemical analysis ImmPRESSTM showed no differences in the expression of...
Treatment of decubitus using moist wound healing method
Zubíková, Markéta ; Rathouská, Jana (advisor) ; Kudláčková, Zděnka (referee)
Bc. Markéta Zubíková Treatment of decubitus using moist wound healing method Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Specialist in laboratory methods The thesis deals with decubitus and method of moist wound healing. The treatment of pressure ulcers is still a hot topic that falls into the problems of modern nursing. Decubitus ulcers are chronic wounds caused by pressure. An important role is prevention, which is based on positioning, rigorous hygiene of the patient, modified bed and use of anti-decubitus aids. Treatment of pressure ulcers is long and complex . Objectives: This thesis compares the quality of care in two hospitals at their wards for chronically ill patients ("LDN 1" in Český Brod and "LDN 2" in the Mnichovo Hradiště) for the year 2015. We assessed the number of patients with decubitus, the most common location of decubitus, risk factors, patient age, usage of moist wound healing method in the hospital and the most common choice of a particular product from this method of healing. The questionnaire survey is focused more on the method of moist wound healing. The aim of this part of the study was to determine whether the method of moist coverage was used and whether the department has enough material to treat patients. Another objective was...
Comparison phenotype with genotype of red blood cells antigens using serologic and microarray techniques
Králová, Jana ; Řeháček, Vít (advisor) ; Rathouská, Jana (referee)
The diploma thesis deals with the erythrocyte antigens genotyping using microarray techniques. It compares the results of microarray methods with the phenotypes obtained by serological techniques. The aim of the theses is to evaluate the degree of identical results (phenotype - genotype) and the applicability of microarray techniques in the immunohematology laboratory. The testing was carried out in the National Reference Laboratory, Institute of Hematology and Blood Transfusion, Prague, in the period January 2014 - December 2015. Methods BLOODchip Reference and ID CORE XT™, Progenika Biopharma S. A., were used for genotyping. Serological determination of phenotypes was performed using Grifols and BioRad gel agglutination techniques. The study encompassed 78 samples of healthy donors of diagnostic red blood cells, 62 patient samples with no possibility to determine the presence of certain antigens by serological methods, and 28 patient samples with the discrepant result of RhD determination. Genotyping of the donors' samples enabled to record variant and weak antigens not detectable by serological methods. Donor samples displayed 100% conformity of serological and genotyping results. Considerable variability in patients' samples was found in comparism of genotyping and serological techniques. The...

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