National Repository of Grey Literature 82 records found  previous11 - 20nextend  jump to record: Search took 0.01 seconds. 
Comparison of subjective and instrumental evaluation of direct agglutination of anticardiolipin antibodies in the diagnosis of syphilis.
Ryšavá, Monika ; Rathouská, Jana (advisor) ; Jílek, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Monika Ryšavá Supervisor: PharmDr. Jana Rathouská, Ph.D. Consultant: MUDr. Hana Zákoucká Title of Thesis: Comparison of subjective and instrumental evaluation of direct agglutination of anticardiolipin antibodies in the diagnosis of syphilis Background: The thesis consisted of a theoretical summary of information about the syphilis disease and its diagnosis. Furthermore, it consisted of an experimental part, the aim of which was to compare the evaluation results of manual and instrumental performance of the nontreponemal test. The evaluation was compared in three groups of patients. The first group consisted of patients with a recent diagnosis of syphilis, the second group consisted of patients already treated for syphilis, and the third group consisted of patients with a negative test for syphilis. Another aim was to evaluate the agreement of sorting the samples into negative and positive, regardless of the antibody titer. The aim was also to evaluate the repeatability and reproducibility of the automatic RPR assessment using the AIX1000 analyzer. Methods: The study was performed at the National Syphilis Reference Laboratory with samples sent for confirmation. 170 samples were...
Carotuximab effects on inflammation during liver fibrosis development
Sharif, Navid ; Rathouská, Jana (advisor) ; Němečková, Ivana (referee)
Chronic liver inflammation is a process that results in the distortion of the liver parenchyma. Constant damage caused by inflammation would lead to formation of fibrotic tissue which can impair the normal liver physiology. Liver fibrosis is one of the common causes of morbidity and mortality worldwide. Non-alcoholic fatty liver disease (NAFLD) is a pathological state that is characterised by excessive accumulation of lipid in the hepatocytes (hepatic steatosis) which can trigger the inflammatory response in the liver. Carotuximab (TRC 105) is a monoclonal antibody that has been used for the treatment of a number of cancers, including hepatocellular carcinoma (HCC). The aim of this study was to investigate the role of carotuximab in liver inflammation in a mouse model of 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-induced liver fibrosis. Eighteen 3-month-old C57BL/6 male mice were recruited in this study. They were divided into three groups: control group (n=6) - fed chow diet for the period of 4 weeks, DDC group (n=6) - fed DDC diet for the period of 4 weeks, DDC group treated with carotuximab (DDC+TRC, n=6) - fed DDC diet for the period of 4 weeks and to that TRC 105 (15mg/kg) was administered. The impact of the diet and TRC 105 on mouse weight, as well as the effect on liver damage (levels...
Nutritional factors in etiopathogenesis of Alzheimer's disease
Park, Hyojung ; Hronek, Miloslav (advisor) ; Rathouská, Jana (referee)
2. ABSTRACT Alzheimer's disease (AD) is a genetic, progressive, neurodegenerative disorder. AD is responsible for the vast majority of dementia cases, and the increasing prevalence of the disease worldwide is a major public health concern. This paper provides evidence from epidemiological studies is mounting that diet and nutrition are among the many potential risk factors being investigated in relation to the development of AD. These factors are closely related to our way of life, what we eat, and the pathophysiology that can modify and prevent in our day-to-day activities. Since there currently exists no effective and proven treatment or preventative measure for AD, increasing focus is being placed on disease prevention in general and the healthy diet in particular. To reduce the probability of developing AD, preventive intervention should be started as early as possible. Due to the neurodegenerative characteristics of this condition, adequate diet must be taken into account as it has both protective and preventative benefits. These include caffeine, isoflavone phytoestrogen, polyphenols, vitamin B, vitamin D, antioxidant vitamins (vitamins C, E, beta-carotene), minerals (avoid copper, iron, and aluminium), unsaturated fatty acids, vitamin B, and vitamin D. There is evidence that the DASH, Mediterranean,...
Benign prostatic hyperplasia
Hrbáčová, Patricie ; Rathouská, Jana (advisor) ; Fikrová, Petra (referee)
Background: Benign prostatic hyperplasia is a relatively common disease affecting men at a later age. It affects a large number of the population and causes discomfort in everyday life. In order to improve the patient's quality of life, it is essential to know the necessary facts about this disease, its origin and effects on the body. Main findings: Under the influence of various factors, prostate cells proliferate and thus enlarge. The enlarged gland then presses on the urethra and causes difficulty urinating. By early and effective diagnosis, we can distinguish benign prostatic hyperplasia from other diseases and also ensure appropriate therapy. Conclusions: Information was sought on the risk factors that lead to this disease, the development of the disease and its consequences for the male organism. The work also describes methods of diagnosis and treatment options depending on the extent of the disease. Keywords: benign prostatic hyperplasia, pathophysiology, diagnostics, treatment
Autoimmune diseases of the thyroid gland
Šlapáková, Tereza ; Rathouská, Jana (advisor) ; Fikrová, Petra (referee)
Background: The aim of this work is to provide a comprehensive overview of thyroid diseases, especially the group of autoimmune diseases, summarize the mechanisms of autoimmunity, physiology of the thyroid gland, introduce selected diseases and the possibilities of their diagnosis or treatment. Main findings: Autoimmune diseases of the thyroid gland are a group of diseases that arise due to a loss of tolerance to their own antigens. However, these mechanisms are not fully studied, many factors are involved. They are more common in women, especially in the working age. Therefore there is a connection with sex hormones. Conclusions: Autoimmune diseases of the thyroid gland are multifactorial diseases that require thorough diagnostics and individualization of therapy. Scientific development and application of the results of new studies to practice enable patients to have a better quality of life. Keywords: thyroid gland, autoimmunity, autoimmune diseases of the thyroid gland
Carotuximab effects on inflammation during liver fibrosis development
Sharif, Navid ; Rathouská, Jana (advisor) ; Němečková, Ivana (referee)
Chronic liver inflammation is a process that results in the distortion of the liver parenchyma. Constant damage caused by inflammation would lead to formation of fibrotic tissue which can impair the normal liver physiology. Liver fibrosis is one of the common causes of morbidity and mortality worldwide. Non-alcoholic fatty liver disease (NAFLD) is a pathological state that is characterised by excessive accumulation of lipid in the hepatocytes (hepatic steatosis) which can trigger the inflammatory response in the liver. Carotuximab (TRC 105) is a monoclonal antibody that has been used for the treatment of a number of cancers, including hepatocellular carcinoma (HCC). The aim of this study was to investigate the role of carotuximab in liver inflammation in a mouse model of 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-induced liver fibrosis. Eighteen 3-month-old C57BL/6 male mice were recruited in this study. They were divided into three groups: control group (n=6) - fed chow diet for the period of 4 weeks, DDC group (n=6) - fed DDC diet for the period of 4 weeks, DDC group treated with carotuximab (DDC+TRC, n=6) - fed DDC diet for the period of 4 weeks and to that TRC 105 (15mg/kg) was administered. The impact of the diet and TRC 105 on mouse weight, as well as the effect on liver damage (levels...
Carotiximab effects on endoglin expression and signaling in liver fibrosis
Mohammadi, SeyedehNiloufar ; Nachtigal, Petr (advisor) ; Rathouská, Jana (referee)
CHARLES UNIVERSITY Faculty of Pharmacy in Hradec Králové Department of Biological and Medical science Carotuximab effect on endoglin expression and signaling in liver fibrosis Diploma thesis Seyedehniloufar Mohammadi Hradec Králové 2022 Supervisor: Prof. PharmDr. Petr Nachtigal, Ph.D. Consultant: M.Sc. Samira Eissazadeh Abstract Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Supervisor: Prof. PharmDr. Petr Nachtigal, Ph.D Student: Seyedehniloufar Mohammadi Title of the thesis: Carotuximab effects on endoglin expression and signaling in liver fibrosis Objective of the thesis Endoglin is a 180 kDa transmembrane glycoprotein that acts as a coreceptor for binding to the Transforming growth factor β (TGFβ) superfamily and is found in two forms in the blood: membrane endoglin (Eng) and soluble endoglin (sEng) circulating in the blood. It has been demonstrated that Eng might play an important role in the process of liver fibrosis, inflammation, and endothelial dysfunction. According to different studies, Eng can have prefibrotic or antifibrotic activity. The precise impact of Eng expression and signalling changes, and sEng levels during liver disorders are still unknown. Carotuximab, TRC105 (chimeric IgG1), is a therapeutic monoclonal antibody that binds to...
JNK3 MAP-kinase localization in the brain of mice with Alzheimer's disease
Kohoutová, Lucie ; Rathouská, Jana (advisor) ; Herink, Josef (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Lucie Kohoutová Supervisor: PharmDr. Jana Rathouská, Ph. D. Specialized supervisor: Dr. Maria Javier Ramirez Title of diploma thesis: JNK3 MAP-kinase localization in the brain of mice with Alzheimer's disease Background: The pathogenesis of Alzheimer's disease (AD) is incompletely understood. Discovery of c-Jun N-terminal kinases (JNKs), involved in regulation of gene expression, inflammation, cell proliferation and apoptosis, may contribute to better understanding of AD pathogenesis. JNK3 is a brain-specific JNK isoform involved in apoptosis of mammalian neurons. Precise localization of JNK3 in the brain still remains unclear. JNK3 silencing achieved by gene knockout had directly decreased beta amyloid (Aβ) levels in previous AD models in mice. We performed an immunohistological study to localize JNK3 gene in the mice brain in order to better focus future gene targeting therapy. Methods: A transgenic mice model of AD (Tg2576) has been used to study the expression of JNK3 by western blotting compared to the wild type. In the same brains localization of pJNK3 in astroglia (GFAP), microglia (Ox42), neurons (NeuN), senile plaques (Aβ) or pTau, using immunohistochemistry staining...
Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice
Krejcarová, Jana ; Rathouská, Jana (advisor) ; Nachtigal, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Diploma Thesis: Western blot analysis of selected inflammatory markers in aorta of apoE/LDLR deficient mice Author: Jana Krejcarová Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: This thesis was focused on the detection and subsequent evaluation of the expression of adhesion molecules ICAM-1, VCAM-1 and COX-2 and P-selectin in mouse aortas. Control and monitored group of mice differed in the genetic material, where monitored group of mice had unfunctional genes for apolipoprotein E and LDL receptor (apoE−/− /LDLR−/− ). Methods: For the study, two months old male mice of the C57BL/6J strain were used. Males were divided into two groups - control and monitored with unfunctional receptor for low- density lipoprotein and for apolipoprotein E. Both groups were fed by a standard diet for rodents. For detection and subsequent quantification of the expression of the markers VCAM-1, ICAM-1, COX-2 and P-selectin, Western blot analysis was used. Levels of soluble ICAM-1 and P-selectin in plasma were assessed by Luminex®. Results: Western blot analysis showed no significant difference in the expression of VCAM-1, ICAM-1, COX-2 and P-selectin between both groups of mice. Using the...
Assessment of chelating properties of 6,7-dihydroxycoumarin
Kolouch, Jan ; Filipský, Tomáš (advisor) ; Rathouská, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Mgr. Jan Kolouch Consultant: PharmDr. Tomáš Filipský, Ph.D. Title of thesis: Assessment of chelating properties of 6,7-dihydroxycoumarin Iron is an indispensable element in the human body. This element plays irreplaceable role in many metabolic processes, such as transport of oxygen and synthesis of DNA. The essential feature of iron is its ability to transport electrons and facilitation of catalytic reactions. These reactions are realized by redox properties of iron, which naturally occurs at two different oxidation states, i.e. Fe2+ and Fe3+ . Nevertheless, this element can catalyze not only beneficial, but also toxic reactions. One of those is Fenton reaction, which results in the formation of reactive oxygen species that are toxic to the surrounding tissue. In this thesis we have focused on the assessment of chelating properties of 6,7- dihydroxycoumarin (67DHC) at various pH conditions (4.5, 5.5, 6.8 and 7.5) using both competitive and direct UV-Vis spectrophotometry. Further, we have evaluated its reducing activity at pH 4.5 and 5.5. By the analysis of UV-Vis absorption spectra we have found out, that 67DHC chelated Fe2+ ions at pH 5.5, 6.8 and 7.5 at the chelating ratio 2:1...

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