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Slitter rewinder control
Růžička, Kamil ; Pásek, Jan (referee) ; Richter, Miloslav (advisor)
This diploma thesis deals with the control of unwinding and winding tension sections on the slitter rewinder for packaging material. The essay was created in collaboration with SOMA company, which provided a machine for testing and measuring. The theoretical part describes the function of bitter rewinder, possible mechanical approaches of control the tension sections, and real mechanics of control on our machine. The next part explores selected hardware and its use on the rewinder. Further chapters are focused on the individual measuring chains, calibration, computations of required quantities and control algorithms. The last part compares the theoretical and measured values and evaluates the results of this essay.
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Greyscale to binary image conversion
Růžička, Kamil ; Horák, Karel (referee) ; Richter, Miloslav (advisor)
In the first part of the bachelor’s thesis are described the basic terms in obtaining and description of a digital image. The next part describes the theory about possibilities of image processing, which are essential for the correct separation of the text from the background, and thus for the correct conversion grayscale image to binary image. Then was performed the analysis of the task with the preparation of data for image processing. Then were applied different methods for converting grayscale to binary images and it was compiled user application. The final step is to evaluate the practical realization of the conversion, the subjective testing of respondents and objektive testing with the OCR software.
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The role of gene AHL20 in initiation and development of lateral roots.
Svobodová, Barbora ; Soukup, Aleš (advisor) ; Růžička, Kamil (referee)
Members of the AHL (AT-hook motif nuclear-localized) gene family have been found in all sequenced plant land species, hence a link between divergence of AHL genes and colonization of terrestrial environments by plants is assumed. One of the adaptations of plants to the terrestrial environment was the development of a root system. Currently there is only a few information about the possible function of AHL genes in root system establishment and development. A typical characteristic of AHL genes is the presence of an AT-hook motif and a highly conserved PPC domain. Many studies have confirmed the involvement of AHL genes in a wide range of plant processes. They are potentially strong regulators of transcription at different levels. They function as transcription factors, interact with other transcription factors through the PPC domain, and are capable of chromatin remodeling through histone modifications. We selected candidate genes based on publicly available transcriptomic data: AHL18, AHL19, AHL20, AHL21, AHL22, AHL23, AHL27, and AHL28 with predicted function in root formation and development. The first aim of this thesis was to investigate possible involvement of AHL genes in lateral root development. For AHL19, AHL20, AHL21, AHL23, AHL27, and AHL28, we examined transcription rates at the root...
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Adenosine methylation (m6A) in plant mRNA
Holoborodko, Anastasiia ; Růžička, Kamil (advisor) ; Náprstková, Alena (referee)
In eukaryotic organisms, there are known more than 200 internal post-transcriptional modifications of RNA. N6-methyladenosine (m6 A) is the most common modification in mRNA. In plants, m6 A affects many aspects of mRNA processing, such as mRNA splicing, alternative polyadenylation, export from the nucleus, its overall stability or translation. Adding, removing, and perceiving of m6 A are handled by protein complexes containing methyltransferases (writers), demethylases (erasers), and m6A-binding proteins (readers), respectively. Dynamic regulations of m6 A have a significant effect on plant development. Also, m6 A exerts its role in response to abiotic stress and viral infection. This thesis summarizes current knowledge on m6A in plants in light of the latest advances in animal experimental models.
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Functions of the exocyst complex in secretion and cell wall biogenesis
Vukašinović, Nemanja ; Synek, Lukáš (advisor) ; Růžička, Kamil (referee) ; Kost, Benedikt (referee)
The mechanical strength of plant tissues and organs can be attributed to specific properties of the cell wall. In many cases, in order to establish their final shape, cells deposit various cell wall materials in a localized manner. This is achieved by highly organized action of the endomembrane system which is essential for biosynthesis and secretion of cell wall proteins and polysaccharides. The exocyst complex is a conserved tethering complex in eukaryotes and it is involved in tethering of secretory vesicles to the sites of secretion at the plasma membrane. In this study, we address several aspects of the plant exocyst complex architecture and cell wall development using molecular biology techniques and advanced confocal microscopy. We demonstrated that two SEC10 exocyst subunits are present in Arabidopsis thaliana and share redundant functions. We also showed that the architecture of the plant exocyst complex shares several structural features with the yeast one. We demonstrated the importance of the functional EXO84b exocyst subunit for normal tracheary element development and showed that the main constituents of the secondary cell walls are deposited normally in exocyst mutants. We described a clear difference in the exocyst microtubule-independent dynamics in epidermal cells vs. cell type...
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Slitter rewinder control
Růžička, Kamil ; Pásek, Jan (referee) ; Richter, Miloslav (advisor)
This diploma thesis deals with the control of unwinding and winding tension sections on the slitter rewinder for packaging material. The essay was created in collaboration with SOMA company, which provided a machine for testing and measuring. The theoretical part describes the function of bitter rewinder, possible mechanical approaches of control the tension sections, and real mechanics of control on our machine. The next part explores selected hardware and its use on the rewinder. Further chapters are focused on the individual measuring chains, calibration, computations of required quantities and control algorithms. The last part compares the theoretical and measured values and evaluates the results of this essay.
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Heterotrimeric G-protein signaling in plants
Šonka, Josef ; Srba, Miroslav (advisor) ; Růžička, Kamil (referee)
G-protein signalling is a key mechanism of cells interaction with the environment. It has a great significance for the coordination of cell division and differentiation in animals, Fungi and Mycetozoa. The research of this type of signalling has also a large consequence in clinical practise. This work summarizes the findings of the importance and principles of heterotrimeric G-proteins signalling in plants which have been published since the first records up to 2012.
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Greyscale to binary image conversion
Růžička, Kamil ; Horák, Karel (referee) ; Richter, Miloslav (advisor)
In the first part of the bachelor’s thesis are described the basic terms in obtaining and description of a digital image. The next part describes the theory about possibilities of image processing, which are essential for the correct separation of the text from the background, and thus for the correct conversion grayscale image to binary image. Then was performed the analysis of the task with the preparation of data for image processing. Then were applied different methods for converting grayscale to binary images and it was compiled user application. The final step is to evaluate the practical realization of the conversion, the subjective testing of respondents and objektive testing with the OCR software.
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