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Determination of amino acids using a coulometric detector with replaceable filling
Prokešová, Edita ; Dejmková, Hana (advisor) ; Fischer, Jan (referee)
This Master Thesis evolves the previously developed construction of coulometric detector with replaceable filling with microspheres of glassy carbon, modifies the filling proces and introduces copper microparticles as the electroactive material. In case of carbon microbeads, different composition of the filling suspension and rapid fillig replacement by means of air bubbles injecting is tested; in case of copper microbeads, also the general construction of the detector was modified and besides the filling process, suitable procedure for the activation of the copper surface and for the cleaning of the detector was seeked. Multivariate statistical analysis was used for the development of method for the determiantion of tyrosine using FIA with electrochemical detection on the detector made of glassy carbon beads. The optimum conditions for the determination of tyrosine were: flow rate 0.6 ml·min-1 , the potential of 1.3 V, the volume ot the injection loop of 50 µl, in a B-R buffer pH 4.5. The limit of detection was determined to 0.5 µmol·dm-3 and the limit of quantification to 1.6 µmol·dm-3 . Phenylalanine was determined by the FIA with the coulometric detection, using detector filled with copper microparticles. optimum conditions for the determination were as follows: flow rate 0.6 ml·min-1 phosphate...
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Determination of amino acids using a coulometric detector with replaceable filling
Prokešová, Edita ; Dejmková, Hana (advisor) ; Fischer, Jan (referee)
This Master Thesis evolves the previously developed construction of coulometric detector with replaceable filling with microspheres of glassy carbon, modifies the filling proces and introduces copper microparticles as the electroactive material. In case of carbon microbeads, different composition of the filling suspension and rapid fillig replacement by means of air bubbles injecting is tested; in case of copper microbeads, also the general construction of the detector was modified and besides the filling process, suitable procedure for the activation of the copper surface and for the cleaning of the detector was seeked. Multivariate statistical analysis was used for the development of method for the determiantion of tyrosine using FIA with electrochemical detection on the detector made of glassy carbon beads. The optimum conditions for the determination of tyrosine were: flow rate 0.6 ml·min-1 , the potential of 1.3 V, the volume ot the injection loop of 50 µl, in a B-R buffer pH 4.5. The limit of detection was determined to 0.5 µmol·dm-3 and the limit of quantification to 1.6 µmol·dm-3 . Phenylalanine was determined by the FIA with the coulometric detection, using detector filled with copper microparticles. optimum conditions for the determination were as follows: flow rate 0.6 ml·min-1 phosphate...
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