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The Utilization of Ultrasound-guided Biopsy in the Management of Ovarian Tumors
Vlasák, Pavel ; Presl, Jiří (advisor) ; Fínek, Jindřich (referee) ; Zikán, Michal (referee)
Study objectives: To compare the diagnostic reliability, accuracy and safety of ultrasound-guided biopsy and ascites puncture in patients with primarily inoperable malignant tumor of the ovary. Study design: Retrospective and prospective data analysis Settings: Department of Obstetrics and Gynecology, Medical Faculty and Teaching Hospital in Pilsen Methods: We retrospectively analyzed a group of patients with primarily inoperable ovarian tumor, who underwent an ascites puncture with subsequent cytologic examination as a part of tumor verification before neoadjuvent chemotherapy commencement. The results were compared to a group of patients, who underwent an ultrasound-guided biopsy with subsequent histopathologic examination for determining of their diagnosis. We compared the adequacy, i.e. possibility to verify biologic nature, origin and histologic subtype of the tumor, accuracy, i.e. agreement between the result of the diagnostic method and final postoperative histology, and safety, i.e. recorded complications of the procedure. To validate the result of this comparative study we prospectively evaluated a group of patients where both methods were used. Results: In the ascites puncture group, a total of 55 procedures were performed. Adequate sample was obtained in in all patients and one severe...
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Biomarkers of epithelial ovarian tumors and of the endometrium
Presl, Jiří ; Novotný, Zdeněk (advisor) ; Špaček, Jiří (referee) ; Fingerová, Helena (referee)
Structured abstract Study objectives: Ovarian carcinoma 1/ comparison of sensitivities among monitored markers CA 125, HE4, CA 19-9, CEA, TK, TPS, MonoTotal 2/ comparison of false positivity of markers CA 125 and HE4 3/ use of CA 125, HE4 and ROMA index in the diagnostics of ovarian carcinoma 4/ use of CA 125 and HE4 in the follow-up of ovarian cancer Endometrial carcinoma 1/ feasibility of use of biomarkers CA125 and HE4 in patients with endometrial cancer in pre- operative management Study design: Retrospective data analysis Settings: Department of Obstetrics and Gynecology, Medical Faculty and Teaching Hospital in Pilsen Patients and Methods: Ovarian cancer 1/ Sensitivity of markers CA 125, HE4, CA 19-9, CEA, TK, TPS, and MonoTotal was assessed in 266 patients - 19 with ovarian cancer and 247 with benign disorders. 2/ False positivity of markers CA125 and HE4 was evaluated in a total of 390 patients with benign diagnoses - 60 women with endometriosis, 70 pregnant patients, 67 patients with ascites, 60 with pleural effusion, 25 with cardiac failure , 80 with renal insufficiency and 28 with hepatic failure. 3/ As a part of this objective we evaluated 552 patients with abnormal pelvic abnormality - 30 women had a histologically confirmed malignant ovarian tumor. Other 522 women had a benign condition. The...
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The Utilization of the Molecular-Biology Method One-Step Nucleic Acid Amplification (OSNA) for Examination of Sentinel Lymph Nodes in Endometrial Cancer Patients
Kosťun, Jan ; Presl, Jiří (advisor) ; Fínek, Jindřich (referee) ; Roztočil, Aleš (referee)
Hypothesis The One-Step Nucleic Acid Amplification method could represent an effective intraoperative tool for detection of metastatic involvement of lymphatic nodes on the level of ultrastaging in endometrial cancer patients. Objective Utilization of the One-Step Nucleic Acid Amplification (OSNA) molecular biology method for the detection of the micrometastatic and macrometastatic involvement of sentinel lymph nodes in endometrial cancer patients. The objective is a comparison with the conclusion of the histopathological ultrastaging of sentinel lymph nodes and a description of the clinical consequences of this method. Methods Patients indicated for the surgical treatment of endometrial cancer underwent the detection of sentinel lymph nodes that was executed using the intracervical application of a tracer. Nodes larger than 5 mm were cut into sections 2 mm thick parallel to the short axis of the node. Odd sections were examined using the OSNA method, while even ones were examined by hematoxylin and eosin (H&E) and immunohistochemical examination to detect cytokeratin 19 antibody (IHC CK19) based on an ultrastaging-relevant protocol. Nodes of the size of 5 mm and smaller were divided into halves along the longitudinal axis with one half being examined using the OSNA method and the other half by...
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The Utilization of the Molecular-Biology Method One-Step Nucleic Acid Amplification (OSNA) for Examination of Sentinel Lymph Nodes in Endometrial Cancer Patients
Kosťun, Jan ; Presl, Jiří (advisor) ; Fínek, Jindřich (referee) ; Roztočil, Aleš (referee)
Hypothesis The One-Step Nucleic Acid Amplification method could represent an effective intraoperative tool for detection of metastatic involvement of lymphatic nodes on the level of ultrastaging in endometrial cancer patients. Objective Utilization of the One-Step Nucleic Acid Amplification (OSNA) molecular biology method for the detection of the micrometastatic and macrometastatic involvement of sentinel lymph nodes in endometrial cancer patients. The objective is a comparison with the conclusion of the histopathological ultrastaging of sentinel lymph nodes and a description of the clinical consequences of this method. Methods Patients indicated for the surgical treatment of endometrial cancer underwent the detection of sentinel lymph nodes that was executed using the intracervical application of a tracer. Nodes larger than 5 mm were cut into sections 2 mm thick parallel to the short axis of the node. Odd sections were examined using the OSNA method, while even ones were examined by hematoxylin and eosin (H&E) and immunohistochemical examination to detect cytokeratin 19 antibody (IHC CK19) based on an ultrastaging-relevant protocol. Nodes of the size of 5 mm and smaller were divided into halves along the longitudinal axis with one half being examined using the OSNA method and the other half by...
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Biomarkers of epithelial ovarian tumors and of the endometrium
Presl, Jiří ; Novotný, Zdeněk (advisor) ; Špaček, Jiří (referee) ; Fingerová, Helena (referee)
Structured abstract Study objectives: Ovarian carcinoma 1/ comparison of sensitivities among monitored markers CA 125, HE4, CA 19-9, CEA, TK, TPS, MonoTotal 2/ comparison of false positivity of markers CA 125 and HE4 3/ use of CA 125, HE4 and ROMA index in the diagnostics of ovarian carcinoma 4/ use of CA 125 and HE4 in the follow-up of ovarian cancer Endometrial carcinoma 1/ feasibility of use of biomarkers CA125 and HE4 in patients with endometrial cancer in pre- operative management Study design: Retrospective data analysis Settings: Department of Obstetrics and Gynecology, Medical Faculty and Teaching Hospital in Pilsen Patients and Methods: Ovarian cancer 1/ Sensitivity of markers CA 125, HE4, CA 19-9, CEA, TK, TPS, and MonoTotal was assessed in 266 patients - 19 with ovarian cancer and 247 with benign disorders. 2/ False positivity of markers CA125 and HE4 was evaluated in a total of 390 patients with benign diagnoses - 60 women with endometriosis, 70 pregnant patients, 67 patients with ascites, 60 with pleural effusion, 25 with cardiac failure , 80 with renal insufficiency and 28 with hepatic failure. 3/ As a part of this objective we evaluated 552 patients with abnormal pelvic abnormality - 30 women had a histologically confirmed malignant ovarian tumor. Other 522 women had a benign condition. The...
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