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Modification of glutathione S-transferase by selected glycating agents
Průchová, Zuzana ; Dršata, Jaroslav (advisor) ; Trnková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Zuzana Průchová Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Title of diploma thesis: Modification of Glutathione S-transferase by selected glycating agents Glycation is a non-enzymatic reaction, which leads to the binding of carbonyl compounds to free amino groups of amino acids, peptides, proteins or other biomolecules. This process is closely related to impaired protein function in diabetes mellitus, during aging, and in neurodegenerative diseases. The main purpose of this thesis was to monitor the impact of several glycating agents on the properties of the enzyme glutathione S-transeferase (GST, EC 2.5.1.18) which plays a key role in the detoxification of many exogenous as well as endogenous compounds. Methylglyoxal (0.5-2 mM), glyceraldehyde (0.5-10 mM), glucose (50 mM), and fructose (50 mM) were selected as glycating agents. The course of protein glycation was evaluated by following methods: GST catalytic activity assessment, determination of primary amino groups, assessment of AGEs formation using fluorescence and Western blotting, generation of high molecular cross-links and aggregates by denaturationg polyacrylamide gel electrophoresis (SDS-PAGE), changes in GST molecular...
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Modification of glutathione S-transferase by selected glycating agents
Průchová, Zuzana ; Dršata, Jaroslav (advisor) ; Trnková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Zuzana Průchová Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Title of diploma thesis: Modification of Glutathione S-transferase by selected glycating agents Glycation is a non-enzymatic reaction, which leads to the binding of carbonyl compounds to free amino groups of amino acids, peptides, proteins or other biomolecules. This process is closely related to impaired protein function in diabetes mellitus, during aging, and in neurodegenerative diseases. The main purpose of this thesis was to monitor the impact of several glycating agents on the properties of the enzyme glutathione S-transeferase (GST, EC 2.5.1.18) which plays a key role in the detoxification of many exogenous as well as endogenous compounds. Methylglyoxal (0.5-2 mM), glyceraldehyde (0.5-10 mM), glucose (50 mM), and fructose (50 mM) were selected as glycating agents. The course of protein glycation was evaluated by following methods: GST catalytic activity assessment, determination of primary amino groups, assessment of AGEs formation using fluorescence and Western blotting, generation of high molecular cross-links and aggregates by denaturationg polyacrylamide gel electrophoresis (SDS-PAGE), changes in GST molecular...
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Concluding a contract resulting from public tender
Průchová, Zuzana ; Horáček, Tomáš (advisor) ; Eichlerová, Kateřina (referee)
The purpose of writing this thesis is to analyse the most important aspects of concluding a contract resulting from public tender. The thesis is composed of three main chapters. The first chapter deals with the definitions of basic terminology in the field of public tender, the purpose of the public procurement procedure and legislation related to the concluding a contract resulting from public tender. The second chapter concerns with the most important and the most interesting aspects of the final phase of public procurement procedure. I point out that the bidder is bound by its offer and that he has the obligation to cooperate when entering into the contract within specified period of time. In this part of the thesis I also describe the procedure of concluding small-scale public contracts and the procedure of concluding a contract resulting from public tender based on framework agreement. I also examine the publication of contracts in relation to the proposal of Register of Contracts Act. The third and final chapter is focused on the possibility of changing the contract resulting from public tender. In this context, I mention the case law of the Court of Justice of the European Union, especially key decision Pressetext. On the basis of this decision new provision related to the prohibition of...
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Modification of glutathione S-transferase by selected glycating agents
Průchová, Zuzana ; Dršata, Jaroslav (advisor) ; Trnková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Zuzana Průchová Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Title of diploma thesis: Modification of Glutathione S-transferase by selected glycating agents Glycation is a non-enzymatic reaction, which leads to the binding of carbonyl compounds to free amino groups of amino acids, peptides, proteins or other biomolecules. This process is closely related to impaired protein function in diabetes mellitus, during aging, and in neurodegenerative diseases. The main purpose of this thesis was to monitor the impact of several glycating agents on the properties of the enzyme glutathione S-transeferase (GST, EC 2.5.1.18) which plays a key role in the detoxification of many exogenous as well as endogenous compounds. Methylglyoxal (0.5-2 mM), glyceraldehyde (0.5-10 mM), glucose (50 mM), and fructose (50 mM) were selected as glycating agents. The course of protein glycation was evaluated by following methods: GST catalytic activity assessment, determination of primary amino groups, assessment of AGEs formation using fluorescence and Western blotting, generation of high molecular cross-links and aggregates by denaturationg polyacrylamide gel electrophoresis (SDS-PAGE), changes in GST molecular...
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Modification of glutathione S-transferase by selected glycating agents
Průchová, Zuzana ; Dršata, Jaroslav (advisor) ; Trnková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Zuzana Průchová Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Title of diploma thesis: Modification of Glutathione S-transferase by selected glycating agents Glycation is a non-enzymatic reaction, which leads to the binding of carbonyl compounds to free amino groups of amino acids, peptides, proteins or other biomolecules. This process is closely related to impaired protein function in diabetes mellitus, during aging, and in neurodegenerative diseases. The main purpose of this thesis was to monitor the impact of several glycating agents on the properties of the enzyme glutathione S-transeferase (GST, EC 2.5.1.18) which plays a key role in the detoxification of many exogenous as well as endogenous compounds. Methylglyoxal (0.5-2 mM), glyceraldehyde (0.5-10 mM), glucose (50 mM), and fructose (50 mM) were selected as glycating agents. The course of protein glycation was evaluated by following methods: GST catalytic activity assessment, determination of primary amino groups, assessment of AGEs formation using fluorescence and Western blotting, generation of high molecular cross-links and aggregates by denaturationg polyacrylamide gel electrophoresis (SDS-PAGE), changes in GST molecular...
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