|
|
Chování psů při interakcích s člověkem
Pospíšilová, Klára
The aim of the bachelor thesis was to find out how dogs behave when interacting with their owner. On the basis of the questionnaire data I found out that the influence of the sex in the command lie down and the leg is conclusive - bitches more often staring into the owner's eyes. In the second part, which was focused on observation, I found many factors that were conclusive. Neutered dogs, dogs living outside the apartment, large dogs, and dogs living with children showed more submissive behavior. Bitches more often stared into the eyes of their owner for more than 5 seconds, and the same behavior was shown by training dogs.
|
|
|
Inhibitors of the eukaryotic translation initiation factor eIF4E1
Pospíšilová, Klára ; Pospíšek, Martin (advisor) ; Roithová, Adriana (referee)
Protein synthesis is one of the most important processes that take place in a cell. Thus there are various mechanisms in a cell that regulate it. If that regulation fails it may lead to serious pathologies. An example of this is an abnormal increase in the production of eukaryotic translation initiation factor 4E1 which occurs in some types of cancer including head and neck squamous cell carcinoma, colorectal cancer, cervical cancer or lung cancer. Enhanced availability of factor 4E1 enables transformed cells to undertake a more intense translation. The expression of individual proteins is not increased to the same extent though. The enhancement in the level of factor 4E1 has a more significant effect on oncogenic proteins. Malignant transformation caused by an incorrect regulation of factor 4E1 can be precluded by an application of compounds that impair the activity of factor 4E1. A summarization of 4E1 inhibitors is the subject of this work.
|
|
| |
|
|
Use of specific inhibitors of carbonic anhydrase IX for fluorecent imaging of cancer cells
Pospíšilová, Klára
Human carbonic anhydrases are metalloenzymes that are involved in many physiological processes in the body, but also play an important role in the pathogenesis of numerous diseases. Under regular conditions, expression of carbonic anhydrase IX (CAIX) is very limited, unlike that of other 14 human carbonic anhydrase isozymes. But in hypoxic tumors this enzyme is highly overexpressed on the cell surface. For this reason, this enzyme represents a good target for therapy and diagnosis of tumors and thus various anti-CAIX monoclonal antibodies and specific inhibitors are being developed. In this work we investigated the possibility to use fluorescent polymer conjugate carrying a CAIX specific inhibitor for florescent labeling of tumor cells. Specific binding of polymer conjugate to different cell lines was investigated by flow cytometry and confocal microscopy. Binding properties of the polymer conjugate was compared to CAIX specific monoclonal antibody M75 and its single-chain fragment scFv M75. Ability of the polymer conjugate to inhibit CAIX enzyme activity was also investigated. For these experiments, recombinant protein CAII was prepared and purified, which was also used for protein crystallization. Tests of inhibitory activities allowed to identify novel inhibitors CAIX with better inhibitory...
|
|
| |
|
|
Historical Changes in the Discourse of Sexuality in the Czech Criminal Law
Pospíšilová, Klára ; Sokolová, Věra (advisor) ; Špondrová, Pavla (referee)
This thesis is focused on historical changes in the social constructions of sexuality, particularly in the czech criminal law. Primary sources for the analysis are criminal codes issued in 1950, 1961 and 2009, including relevant amendments. The aim of the research is (by use the in-depth textual analysis and critical gender position) to highlight the historical changeability of the discourse of sexuality and to uncover related meanings in all texts used for the analysis. I also focus on the specific way, how it comes to the social construction of gender and thus which representation of masculinity and femininity is possible to see in the criminal law related to the defined criminal acts related to the sexuality. Criminal codes are understood as the official, universally applicable documents. How the czech criminal law contributes to the social control of sexuality? The most imporant keywords are: gender, discourse, language, criminal law, sexuality.
|
|
|
Use of specific inhibitors of carbonic anhydrase IX for fluorecent imaging of cancer cells
Pospíšilová, Klára ; Maloy Řezáčová, Pavlína (advisor) ; Novotný, Marian (referee)
Human carbonic anhydrases are metalloenzymes that are involved in many physiological processes in the body, but also play an important role in the pathogenesis of numerous diseases. Under regular conditions, expression of carbonic anhydrase IX (CAIX) is very limited, unlike that of other 14 human carbonic anhydrase isozymes. But in hypoxic tumors this enzyme is highly overexpressed on the cell surface. For this reason, this enzyme represents a good target for therapy and diagnosis of tumors and thus various anti-CAIX monoclonal antibodies and specific inhibitors are being developed. In this work we investigated the possibility to use fluorescent polymer conjugate carrying a CAIX specific inhibitor for florescent labeling of tumor cells. Specific binding of polymer conjugate to different cell lines was investigated by flow cytometry and confocal microscopy. Binding properties of the polymer conjugate was compared to CAIX specific monoclonal antibody M75 and its single-chain fragment scFv M75. Ability of the polymer conjugate to inhibit CAIX enzyme activity was also investigated. For these experiments, recombinant protein CAII was prepared and purified, which was also used for protein crystallization. Tests of inhibitory activities allowed to identify novel inhibitors CAIX with better inhibitory...
|
|
| |
|
| |
|
| |
guest ::
RSS feed.