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The usage of qi gong for training of perceiving body schema
Pospíšilová, Eva ; Pánek, David (advisor) ; Véle, František (referee)
Title: The usage of qi-gong for training of perceiving body schema Summary: The goal of the work is to prove the presence of alpha activity in the electroencencefalographic record throughout the duration of the exercise qi-gong with open eyes and closed eyes, and to evaluate changes in the distribution of the scalp alpha activity with native EEG before and after the exercise. The observed research file was created from five probands between the ages of twenty-seven to fifty-two, which all practiced qi-gong for a duration of at least twelve months. The results showed the presence of alpha activity during exercising qi-gong with closed eyes in four probands, and in three probands there was also a present alpha activity during the exercise of qi-gong with open eyes. Furthermore was proven that the change in distribution of alpha activity during exercise of qi-gong with open eyes was from parietooccipital regions going temporo-frontally in comparison with the exercise of qi- gong with closed eyes and native EEG before and after exercise. The acquired results support in literature the described change of generators of alpha activity localized in the deeper structures of the brain. This process is connected with the decreased activity of the cerebral cortex with an increase in the particular limbic structures....
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Adenylate cyclase toxin of bacteria Bordetella pertussis: mechanism of potassium efflux from macrophages
Pospíšilová, Eva ; Mašín, Jiří (advisor)
The adenylate cyclase toxin-hemolysin (ACT or CyaA) is a key virulence factor of Bordetella pertussis, the agent of the human respiratory disease whooping cough. CyaA penetrates phagocytes expressing the CD11b/CD18 integrin and exhibits two different activities. One toxin conformer oligomerizes in cell membrane and permeabilizes it by forming small cation-selective pores. Another toxin conformer appears to act as monomer. It forms a calcium influx path in the membrane, concomitantly with translocating into cells the adenylate cyclase enzyme domain that binds calmodulin and catalyzes unregulated conversion of cytosolic ATP into cAMP. We show here that CyaA causes efflux of K+ from CD11b+ cells by a mechanism that requires binding of CyaA to integrin CD11b/CD18 and permeabilization of the cellular membrane by pore-forming conformer of CyaA. Intact CyaA and the enzymatically inactive CyaA-AC- toxoid unable to generate cAMP produced the same kinetics of K+ efflux showing that elevation or signaling of cAMP had no role in this activity. The truncated CyaA variant (CyaA-AC) devoid of its invasive AC domain failed to promote K+ efflux despite a normal pore forming activity on erythrocyte and artificial membranes. However, binding of the MAb 3D1, which recognizes the distal segment of the AC domain...
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název v anglickém jazyce není uveden
Pospíšilová, Eva ; Paleček, Jiří (advisor) ; Kršiak, Miloslav (referee) ; Rokyta, Richard (referee)
Tissue injury leads to increased sensitivity to noxious and innocuous stimuli due to mechanisms of peripheral sensitisation of primary nociceptors and central sensitization of neurons in the spinal dorsal horns. The subpopulation of capsaicin - sensitive sensory neurons plays an important role in this process. The capsaicin - sensitive neurons express capsaicin TRPV1 receptors (transient receptor potential 1) on their peripheral and central terminals, local high concentration capsaicin treatment can induce regional destruction of these endings. It is well estabilished that TRPV1 receptors play the key role in neural transmission of nociceptive information and its modulation. The aims of this study were to investigate the role of capsaicin - sensitive primary afferent fibers and the involvement of peripheral and central TRPV1 receptors in the development of hypersensitivity after surgical tissue trauma, to test the effect of high concentration capsaicin and the specific TRPV1 antagonist treatment on postoperative pain and to enlighten the function of central TRPV1 receptors in the neural mechanisms of nociception. Using behavioral testing methods, the responses to mechanical (Von Frey filaments) and thermal stimuli (radiant heat source) were tested on the rat plantar incision model of surgical pain...
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Adenylate cyclase toxin of bacteria Bordetella pertussis: mechanism of potassium efflux from macrophages
Pospíšilová, Eva ; Konopásek, Ivo (referee) ; Mašín, Jiří (advisor)
The adenylate cyclase toxin-hemolysin (ACT or CyaA) is a key virulence factor of Bordetella pertussis, the agent of the human respiratory disease whooping cough. CyaA penetrates phagocytes expressing the CD11b/CD18 integrin and exhibits two different activities. One toxin conformer oligomerizes in cell membrane and permeabilizes it by forming small cation-selective pores. Another toxin conformer appears to act as monomer. It forms a calcium influx path in the membrane, concomitantly with translocating into cells the adenylate cyclase enzyme domain that binds calmodulin and catalyzes unregulated conversion of cytosolic ATP into cAMP. We show here that CyaA causes efflux of K+ from CD11b+ cells by a mechanism that requires binding of CyaA to integrin CD11b/CD18 and permeabilization of the cellular membrane by pore-forming conformer of CyaA. Intact CyaA and the enzymatically inactive CyaA-AC- toxoid unable to generate cAMP produced the same kinetics of K+ efflux showing that elevation or signaling of cAMP had no role in this activity. The truncated CyaA variant (CyaA-AC) devoid of its invasive AC domain failed to promote K+ efflux despite a normal pore forming activity on erythrocyte and artificial membranes. However, binding of the MAb 3D1, which recognizes the distal segment of the AC domain...
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Nursing practices internal medicine in context with the development.
POSPÍŠILOVÁ, Eva
Nursing methods are understood as a collection of knowledge and instructions about how to perform nursing in order to achieve the most effective nursing care. It's not a set of fixed rules. Their development relies on the development of medicine and technology. They are in accordance with medicinal and technological knowledge. The goal of this thesis is to map the development of certain nursing methods in internal departments from the second half of the 19th century to the present day. It has been a qualitative research carried out by studying and analyzing written documents. This thesis was based on searching, sorting and consequent processing of historical archived publications and contemporary professional sources related to this topic. The thesis is divided into two parts. The first part deals with the history of nursing. It contains a brief description of the beginning of nursing along with the factors which have influenced nursing. Foundations of modern nursing have formed thanks to Florence Nightingale, who also influenced the development of nursing in the Czech lands. The history of Czech nursing is mostly concentrated around the period of the world wars. The second part is dedicated to specific nursing activities concerning healthy living spaces, rooms for patients, patients? beds and their modifications, care of patients? hygiene and monitoring the vital functions. The timeframe begins at the first half of the 19th century due to the establishment of real nursing professions at that time. Individual stages are ten years long. They indicate complex development of specific nursing methods from their beginnings to the present day. Mapping the historical development of certain nursing methods can contribute to the modern nurse's work. Past knowledge can help them to avoid mistakes and use of outdated methods. My thesis may also lead to a reflection of current methods, which may be replaced by more advanced ones.
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Možnosti a způsoby využití kalů a sedimentů z ČOV
ENVISAN-GEM a.s., Praha ; AGRO-EKO, Ostrava ; Výzkumný ústav vodohospodářský T.G. Masaryka, Praha ; Petrášková, L ; Vosáhlová, Simona ; Kyclt, Robin ; Holuša, Václav ; Pospíšilová, Eva ; Sýkora, Karel ; Sedláček, Miroslav ; Michalová, Marie
Současný stav v přípravě a diskusi o výhledovém nakládání s kaly a bioodpady v EU. Současný stav nakládání s čistírenskými kaly v ČR. Údaje o produkci a nakládání s kaly, produkce ostatních odpadů z ČOV v ČR. Charakteristika technického stavu kalového hospodářství v ČR. Prognóza vývoje následného využití/odstranění kalu z ČOV. Odhad investičních nákladů na kalové hospodářství s vazbou na nakládání s kalem podle zákona o odpadech. Nové trendy v nakládání s kaly. Dosahovaná kvalita kalů ve vztahu k limitům rizikových látek a platné legislativy.
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Možnosti a způsoby využití kalů a sedimentů z ČOV
AGRO-EKO, Ostrava ; ASLAB ; Ústřední kontrolní a zkušební ústav zemědělský Brno, oddělení agrochemie, půdy a výživy rostlin, Plzeň ; Státní zdravotní ústav, Praha ; Výzkumný ústav vodohospodářský T.G. Masaryka, Praha ; Brožková, Zuzana ; Holuša, Václav ; Suk, Michal ; Sedláček, Miroslav ; Geuss, Erik ; Pospíšilová, Eva ; Nižňanská, A. ; Koruna, Ivan ; Sezima, Tomáš ; Chvátal, Vladislav ; Matějů, Ladislava ; Michalová, Marie
V roce 2002 byly vyhodnoceny schopnosti hlavních producentů kalu využít kaly v zemědělství z hlediska technologie, byl zjišťován praktický dopad nakládání s kaly z komunálních ČOV ve vybraných zemích Evropy z hlediska hodnocení nebezpečných vlastností - zejména infekčnosti, provedeno orientační ekonomické posouzení celého procesu využívání a odstraňování čistírenských kalů v ČR, byly vybrány laboratoře pro provádění rozborů kalů, poloprovozně ověřena recyklace čistírenských kalů, navrženo hodnocení vlivu aplikace kalů z komunálních ČOV na zemědělskou půdu a rostlinnou produkci ve vztahu na půdní registr spolu s konkretizací potenciálních rizik s ohledem na plnění produkčních a ekologických funkcí půdy (návrh a rozpracování), proběhla příprava na budoucí úpravy vyhlášky podle výhledové novely Směrnice č. 86/278/EEC v ČR a hodnocení zejména zdravotního rizika při dalším využití kalů (kompostování, rekultivace, energetické využití) včetně stanovení mikrobiologických kritérií.
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