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The comparison of different types of stationary phases in HPLC analysis of medicament Diclofenac emulgel
Poláčková, Jitka ; Matysová, Ludmila (advisor) ; Karlíček, Rolf (referee)
The aim of this rigorous work is a comparison of different types of stationary phases for the analysis of the drug Diclofenac emulgel. It is necessary to take into account the many specialized materials from the pharmacopoeias, ICH guidelines and professional scientific journals in solution of the development of HPLC methodology, validation and choice of stationary phase analytical columns. Several types of stationary phases were tested, with the testing of conditions of the separation of active substances from the pharmaceutical formulation - diclofenac emulgel. The best results were placed on Discovery HS-F5 (15 cm x 4.6 mm, 5 m), time analysis was less than 4 minutes; Zorbax SB - CN (4.6 x 150 mm, 5 m) time of analysis was less than 5 min; Chromolith Performance RP - 18e (100 - 3 mm) time analysis was less than 3 minutes.
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Development and validation of method for determination of clotrimazole in cream using HPLC
Poláčková, Jitka ; Matysová, Ludmila (advisor) ; Chocholoušová Havlíková, Lucie (referee)
The topic of my work was to optimalize and to validate the method for determination of capacity of the Clotrimazol, degradeted product (2-chlorophenyl) diphenylmethanol and conservations (methylparaben and propylparaben) in the preparation Clotrimazol ointment. My effort for this work was to develop the method that could half for total separation the particular defined substance in this sample, in the acceptable time, to find inside standard, to optimalize the conditions of separation and the method verify. The optimum chromatographic conditions were found on column Zorbax SB - Phenyl, 3,5 μm, 4,6 x 75 mm in the flow of mobile phase 0,5 ml per minute and wave - length 210 nm. Composition of the mobile phase was acetonitrile - water (55:45), pH water component was modified by acid phosphoric 5 % on 3,2. If these conditions are used, this method is validating. The testing parameters were accuracy, correctness, linearity, selectivity, robustness, detection and quantitative limit and test if the chromatographic system is acceptable.
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Development and validation of method for determination of clotrimazole in cream using HPLC
Poláčková, Jitka ; Matysová, Ludmila (advisor) ; Chocholoušová Havlíková, Lucie (referee)
The topic of my work was to optimalize and to validate the method for determination of capacity of the Clotrimazol, degradeted product (2-chlorophenyl) diphenylmethanol and conservations (methylparaben and propylparaben) in the preparation Clotrimazol ointment. My effort for this work was to develop the method that could half for total separation the particular defined substance in this sample, in the acceptable time, to find inside standard, to optimalize the conditions of separation and the method verify. The optimum chromatographic conditions were found on column Zorbax SB - Phenyl, 3,5 μm, 4,6 x 75 mm in the flow of mobile phase 0,5 ml per minute and wave - length 210 nm. Composition of the mobile phase was acetonitrile - water (55:45), pH water component was modified by acid phosphoric 5 % on 3,2. If these conditions are used, this method is validating. The testing parameters were accuracy, correctness, linearity, selectivity, robustness, detection and quantitative limit and test if the chromatographic system is acceptable.
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The comparison of different types of stationary phases in HPLC analysis of medicament Diclofenac emulgel
Poláčková, Jitka ; Karlíček, Rolf (referee) ; Matysová, Ludmila (advisor)
The aim of this rigorous work is a comparison of different types of stationary phases for the analysis of the drug Diclofenac emulgel. It is necessary to take into account the many specialized materials from the pharmacopoeias, ICH guidelines and professional scientific journals in solution of the development of HPLC methodology, validation and choice of stationary phase analytical columns. Several types of stationary phases were tested, with the testing of conditions of the separation of active substances from the pharmaceutical formulation - diclofenac emulgel. The best results were placed on Discovery HS-F5 (15 cm x 4.6 mm, 5 m), time analysis was less than 4 minutes; Zorbax SB - CN (4.6 x 150 mm, 5 m) time of analysis was less than 5 min; Chromolith Performance RP - 18e (100 - 3 mm) time analysis was less than 3 minutes.
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The influence of foregoing crop on oats yield and quality
POLÁČKOVÁ, Jitka
Nowadays trend in Europe is returning to harvesting of traditional feeding crops. One of these crops is oat which has been universally used since time immemorial. Even today oats are processing and using in food industry and as a feed for farm animals. Further using of oats is in cosmetics and pharmaceutical industry. Oats contain high amount of proteins and fats, beta-glukan and mineral elements. Oats include vitamins B and E, lecitin, niacin and antioxidants. Research was executing on fields of University of South Bohemia in České Budějovice during one year. An impact on crop yield and quality of oats was observed in dissertation. There were used three crops corn, rape and cereal (spring wheat) in research. After these crops were sown ten varieties of oats, four naked and six husked varieties. At these varieties were observed an impact on crop yield and quality of oats. Evaluated characters were monitored during the vegetation, pre-harvest and post-harvest. There were observed germination and methane, height of vegetation, the degree of lodging oats, the number of lat m2, weeds, diseases and pests, after harvest was determine harvested grain yield, moisture and density, number of grains per panicle, thousand grain weight and grain on a network share during the vegetation. From the measured values follows that the best crop in year 2012 was corn and the best results were achieved by variety Abel, Avenuda, Atego, Pogon, Salo.
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