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Marking of rainbow trout with Alizarin
Halačka, Karel ; Mareš, J. ; Poštulková, E. ; Malý, O.
Method of using alizarin red (ARS) for mass-marking of rainbow trout was verified and optimized. Based on the results, it was possible to create methodological recommendations for marking individuals of rainbow trout using ARS: 1. Marking can be used shortly after hatching (around 14 days) since the fin rays are sufficiently developed or calcified. The standard application time and solution concentration (1 hour, 150 mg/l) can be used if the expected detection time of marker is around 4-5 weeks. It is necessary to increase the application time on 3 hours in order to distinguish individuals 2-3 months after marking. There was no evidence of a negative effect of the dye on rainbow trout individuals (mortality) or a change in chemistry (pH, conductivity, oxygen saturation of water) within standard concentrations and application time and economic parameters. The use of Alizarin red can significantly reduce financial (around 1 CZK/individual) and time requirements for fish marking. When compared with other types of marking, costs can be reduced to 10 percent (or less) and time-consuming (meaning labor costs) can be reduced to several percent. Another advantage that is difficult to quantify is benefit from information obtained by marking on the farms.
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Ekotoxicita látek využívaných v rybářském sektoru =: Ecotoxicity of substances used in fisheries /
Poštulková, Eva
The aim of this dissertation thesis was to ascertain the effects of a substances which are used or can be used in fisheries. The first group of tested substances were algaecides, the most common tool used for algae and cyanobacteria elimination. The second group consists of organic pigments, which can be used for fish mass-marking from early stages. The selection of the test organism for the toxicity tests was performed to represent the individual trophic levels of aquatic ecosystems: primary producers, zooplankton and fish. For acute toxicity tests and inhibition tests were used these algaecides: Guanicide, Pelargonic Acid, Polyaluminiumchloride (PAX 18), Polyhexamethylene Guanidine (PHMG), and Terbutryn. For the organic dyes testing was selected fluorescent dye Alizarin Red S and Alizarin Complexone. Algaecides were used in inhibition tests for green algae (Desmodesmus communis, Parachlorella kessleri) and cyanobacteria (Anabaena sp.), in immobilization test for zooplankton (Daphnia magna) and acute tests for fish (Danio rerio). The organic dyes were tested on fish for determination of effective and safe concentration.
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Toxic effect of fluorescence pigment on zebra fish (Danio rerio)
Poštulková, E. ; Mareš, J. ; Halačka, Karel ; Kopp, R.
The aim of the study was to determine the toxic effects of organic pigments Alizarin Red S and Alizarin Complexone on zebra fish (Danio rerio). For short-term acute toxicity tests on zebra fish concentration of 150; 300 and 600 mg/L were chosen for both dyes. Toxic effect of dyes was observed even in the variant with 10 g/L of sodium chloride. Addition of sodium chloride increases the deposition of dyes in the bone structures of the fish. LC50 values were analyzed graphically by using probit analysis. There was no mortality during the acute toxicity test with Alizarin Red S even at the highest concentration. Toxicity value (72hLC50) for zebra fish with a combination of Alizarin Red S + 10 g/L of sodium chloride is 546.42 mg/L. Mortality for Alizarin Complexone was 100% in 24 hours at\nconcentrations of 300 and 600 mg/L, with concentrations of 150 mg/L there was no mortality. In Alizarin Complexone supplemented with 10 g/L of sodium chloride was 100% mortality at all\nconcentrations up to 72 hours.
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