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Radiolabelled receprot-specific peptides for cancer diagnosis and therapy
Parýzková, Barbora ; Bárta, Pavel (advisor) ; Trejtnar, František (referee)
Charles University Fakulty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Bc. Barbora Parýzková Supervisor: Mgr. Pavel Bárta, Ph.D. Title of diploma thesis: Radiolabelled receptor-specific peptides for cancer diagnosis and therapy. The presented diploma thesis is based on literature review and it deals with receptor- specific radioactive labeled peptides and their usage in the cancer diagnostic and treatment. It focuses on general knowledge about radiopharmaceuticals, further on the descriptions of either diagnostic or therapeutic radionuclides as well as on currently used chelating agents. The particular attention is paid to peptides like somatostatin and cholecystokinin and their usability in Nuclear Medicine for the imaging and treatment of certain malignancies. The other stated peptides in this diploma thesis are bombesin, vasoactive intestinal peptide and alpha melanocyte stimulating hormone. Keywords: receptor-specific peptides, radiolabelling, radionuclides for diagnosis and therapy, radiotherapy, radiodiagnostics, octreotide.
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Diabetes mellitus versus celiac disease, genetic testing of the above mentioned diseases using the Protrans kit
PARÝZKOVÁ, Barbora
Celiac disease is an autoimmune disease affecting the mucous membrane of small intestine. The development of this disease is affected by genetic predispositions, but the onset of this disease is also influenced by environmental factors. Genetic predisposition is primarily attributed to HLA system, HLA-DQ2 and HLA-DQ8 in particular. Celiac disease is characterized by many symptoms therefore its diagnosis is difficult and stays undiagnosed in many cases. The aim of the theoretical part of this bachelor´s thesis was to summarize the knowledge of this topic regarding celiac disease and diabetes type 1. as an associated disease, possibly coexisting with celiac disease. The work, dealt with celiac disease occurrence, its pathogenesis, symptoms and types and possible diagnostic approaches and effective treatment. In the end of the theoretical part it also mentions possible associated diseases with celiac disease. The practical part of my work took part in the genetic laboratory GENLABS, where the examination of genetic predispositions to celiac disease were conducted, based on HLA typing using commercially available Protrans kit. The aim of the practical part was to master the diagnostic method of determining the risk HLA haplotype. Further, mastering the DNA isolation method, using peripheral blood and buccal smear, preparation and execution of Polymerase Chain Reaction (PCR) and detection of created PCR products using gel electrophoresis on agarose gel. The Protrans kit is not used for determination of the predispositions to celiac disease in GENLABS any more, it was replaced by real-time PCR method, which is less burdensome and faster and less financially demanding, than the previous one.
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