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The Story of Teamwork and the Birth of the FFP* (Filter Backpack)
Fraenkl, M. ; Krbal, M. ; Houdek, J. ; Zmrhalová, Z. ; Prokeš, B. ; Hejda, P. ; Slang, S. ; Přikryl, J. ; Ondráček, Jakub ; Makeš, Otakar ; Kostyk, Juraj ; Nasadil, P. ; Malčík, P. ; Ždímal, Vladimír ; Vlček, M.
Soon after the outbreak of the coronavirus crisis in the Czech Republic and the first lockdown (2020), we enthusiastically decided to fight the coronavirus with scientific \nmeans. Originally materials engineers, we decided to develop an effective protective respiratory device, which was in short supply at the time. We soon found out, that every textile material (handkerchief 10%) has a certain ability to catch an aerosol particle carrying the corona virus, the slower the aerosol particle (d<300 nm) passes through the filter, the greater the chance it has of being caught, with the thickness of the filter, the amount of passed particles decreases exponentially and breathing resistance increases linearly. On this basis, we decided to experiment with a large-area filter placed on the user's back (it wouldn't fit anywhere else) and commercially available textile filter material.
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Chemical composition and sources of atmospheric aerosols at the Frýdlant background station
Lhotka, Radek ; Pokorná, Petra ; Vodička, Petr ; Zíková, Naděžda ; Ondráček, Jakub ; Arora, S. ; Poulain, L. ; Hermann, H. ; Schwarz, Jaroslav ; Ždímal, Vladimír
This study assesses the variability of organic aerosol (OA) sources monitored at the rural background site Frýdlant. Non-refractory PM1 was evaluated in two seasons of\n2021. The positive matrix factorization with the multi-linear engine was used to determine the sources of OA at Frýdlant site, with four factors resolved both in winter and\nsummer.
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Cleaning library documents with a two-phase spray.
Mašková, Ludmila ; Smolík, Jiří ; Vávrová, P. ; Neoralová, J. ; Součková, M. ; Novotná, D. ; Jandová, Věra ; Ondráček, Jakub ; Ondráčková, Lucie ; Křížová, T. ; Kocová, K.
The cleaning of particles from library materials (paper, textile, and collagen materials) using a high-speed CO2 snow jet was investigated. The measurements included\ndetermination of the cleaning efficiency, and evaluation of possible adverse effects. The method was compared with nitrogen jet cleaning. The results showed that the CO2 snow\njet is able to effectively remove particles from the surfaces. Any adverse effects were not observed at paper and textile. However, application on collagen materials caused\ndegradation of the surface.
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Males-females differences in the spectrum of chromosomal aberrations in the group of nanocomposites production workers
Rössnerová, Andrea ; Pelcová, D. ; Ždímal, Vladimír ; Elzeinova, Fatima ; Margaryan, Hasmik ; Chvojková, Irena ; Topinka, Jan ; Schwarz, Jaroslav ; Ondráček, Jakub ; Koštejn, Martin ; Komarc, M. ; Vlčková, Š. ; Fenclová, Z. ; Lischková, L. ; Dvořáčková, Š. ; Rössner ml., Pavel
An increase in the use of nanomaterials (NM) has been witnessed in many areas of human life. Therefore, assessment of genotoxicity of NM and nanoparticles (NP) is one of the main objectives of genetic toxicology. Despite this fact, human cytogenetic studies following the exposure to NP are still rare. Moreover, no relevant information on possible differences in sensitivity to NP related to gender is available.\n\nIn this study we periodically (in September 2016, 2017 and 2018; pre-shift and post-shift each year) analyzed a group of workers (both genders), working long time in nanocomposites research, and matched controls. Aerosol exposure monitoring of particulate matter including nano-sized fractions was carried out during working shift. Micronucleus assay using Human Pan Centromeric probes, was applied to distinguish, besides the frequency of total MN in binucleated cells (BNC), also other types of chromosomal damage (losses and breaks). Moreover, whole-chromosome painting (WCP) for autosome #1 and both gonosomes (X and Y) were applied in third sampling period (2018) with the aim to identify the particular structural and numerical chromosomal aberrations.\n\nObtained results showed: (i) differences in the risk of exposure to NP related to individual working processes (welding, smelting and machining); (ii) differences in chemical composition of nano-fraction; (iii) no effect of chronic exposure of NP (total MN) opposite to significant effect of acute exposure; (iv) gender-related DNA damage differences (females seem to be more sensitive to chromosomal losses). Additional data from WCP suggested increased frequency of numerical aberrations in gonosomes.
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Personal exposure measurement during dental nanocomposite grinding
Ždímal, Vladimír ; Ondráčková, Lucie ; Ondráček, Jakub ; Schwarz, Jaroslav ; Bradna, P. ; Roubíčková, A. ; Pelclová, D. ; Rössnerová, Andrea
The purpose of this study was to measure the personal exposure of each participant of the study and to compare the results with those of static monitoring. Personal nanoparticle sam-plers (PENS), which can simultaneously detect both nanoparticles (PM0.1) and respirable parti-cles (PMA), were used to determine personal exposure (Tsai et al., 2012). Area monitoring in-cluded measurement of mass concentrations using the Berner Low Pressure Impactor (BLPI 25/0.018 /2, Hauke GmbH, Gmunden, Austria) and the Low Volume Sampler (LV5, Sven Leckel Ingenieurbüro GmbH, Germany). The number concentrations and their size distributions were measured with the Scanning Mobility Particle Sizer (5MP5 3936, T5I Inc., USA) and the Aerody-namic Particle Sizer (APS 3321, TSI Inc., USA). Measurements with all of the above- mentioned instruments were performed in four shifts with six participants per shift. Each participant milled for 10 minutes and then remained in the room until the group finished the session, so the total exposure lasted about 70 minutes. Due to the high content of filler nanoparticles, the nanocom-posite Filtek Ultimate (body A2, 3M ESPE, USA) was selected for these measurements.
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Methodology for cleaning paper, textiles and collagen materials using the two-phase spray of CO2 snow particles in the carrier gas stream.
Mašková, Ludmila ; Smolík, Jiří ; Vávrová, P. ; Součková, M. ; Neoralová, J. ; Novotná, D. ; Jandová, Věra ; Ondráček, Jakub ; Ondráčková, Lucie ; Křížová, T. ; Kocová, K.
The aim of this methodology was to create a set of procedures and recommendations for library and archive staff or restorers, using which it is possible to remove dust particles from the surface of paper, textiles and collagen materials using the two-phase spray of CO2 snow particles in the carrier gas. The result is an effective and safe alternative to existing cleaning methods. Furthermore, the research aimed to compare the results of this type of treatment with commonly used mechanical cleaning procedures for library collections and archival documents and to analyse in detail the potential use of the new method.
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Methodology of cleaning paper, textiles and collagen materials using thr two-phase spray of CO2 snow particles in the carrier gas stream
Mašková, Ludmila ; Smolík, Jiří ; Vávrová, Petra ; Součková, Magda ; Neoralová, Jitka ; Novotná, Dana ; Jandová, Věra ; Ondráček, Jakub ; Ondráčková, Lucie ; Křížová, Tereza ; Kocová, Kateřina
Cílem této metodiky bylo vytvořit soubor postupů a doporučení pro pracovníky knihoven a archivů či pro restaurátory, s jejichž využitím je možné pomocí dvoufázového spreje sněhových částic CO2 v nosném plynu odstraňovat prachové částice z povrchu papíru, textilu a kolagenních materiálů. Výsledkem je efektivní a bezpečná alternativa ke stávajícím metodám čištění.
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Experimental Methods to Study Aerosol Nanoparticles.
Ždímal, Vladimír ; Schwarz, Jaroslav ; Ondráčková, Lucie ; Ondráček, Jakub
During the last few decades, the experimental possibilities of studying aerosol particles have grown enormously. Not only is it possible to determine the particle size distribution in different metrics, not only can the chemical composition of the size-resolved aerosol be determined, but methods have been developed over the last two decades that allow all of these tasks to be handled in real time. These methods stem from several basic physical principles: molecular diffusion based on Brownian motion, electrostatic separation of particles with predictable charge, condensational growth of particles, gravitational settling, acceleration of particles in nozzles, inertial impaction, and light scattering on particles.\nHowever, if we are specifically interested in separating particles smaller than 100 nanometers in diameter, the choice of experimental methods would be substantially reduced. In fact, we have only four physical principles that can be utilized in this size range with reasonable degree of uncertainty: Brownian motion, electrostatics, impaction and condensation. For the determination of the chemical composition in a given size range, the most commonly used is a combination of physical / chemical ionization with mass detection, however, the range of quantifiable substances is greatly limited.\nRecently, exposure monitoring of workers in the production of engineered nanoparticles has become increasingly important. Here, the task is further complicated by the fact that it is necessary to sample directly from the vicinity of the worker's mouth to determine personal exposure. As far as the collection of nanoparticles in the respiratory zone is concerned, there is not yet a great choice of options, and experimental methods are still being developed and tested. A promising alternative is a stationary measurement, where state-of-the-art aerosol spectrometers are located close to the working space of the personnel, so that the actual exposure of the worker can be estimated. In this case, however, it is necessary to calibrate the on-line instruments by comparison with simultaneous personal collection.\n
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