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Possibilities of UCP2 (uncoupling protein 2) induction in rat hepatocytes in in vivo conditions
Bolehovská, Radka ; Červinková, Zuzana (advisor) ; Kalous, Martin (referee) ; Novotný, Dalibor (referee)
Possibilities of UCP2 (uncoupling protein 2) induction in rat hepatocytes in in vivo conditions Introduction Uncoupling protein 2, discovered in 1997, is the first described homologue of uncoupling protein 1. Uncoupling proteins increase the permeability of inner mitochondrial membrane for protons, decrease the efficiency of energy conversion, inhibit the ATP synthesis and stimulate energy release in form of heat. Uncoupling proteins also increase the substrate oxidation and reduce production of reactive oxygen species in mitochondria. Objective, material and method The aim of this study was to establish and optimised the quntitative real-time PCR for detection of UCP2 mRNA expression kinetics in rat liver tissue. The present study was conducted to assess the effects of acute and chronic treatment with triiodothyronine and the effect of partial hepatectomy on liver uncoupling protein 2 mRNA levels in male Wistar rats. Results Intraperitoneal injection of one dose of triiodothyronine (200 μg/kg rat body weight) increased mRNA expression of uncoupling protein 2 in liver tissue almost 2-fold (P < 0.01 vs. control group) in rats 12 hours after T3 administration. Concentrations of total triiodothyronine and free triiodothyronine in serum were increased 122-fold and 77-fold (p < 0.001), respectively....
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Analysis and Modeling of Macroeconomic in the Czech Republic
Novotný, Dalibor ; Bartošová, Jitka (advisor) ; Voráček, Jan (referee)
Main goal of my dissertation was take a close look at progress of several economics indicators from 1993. I also decided to analyse key factors wich mainly influence these ecnomics indicators. This work is based on classical model IS-LM-BP which is most suitable because of its logic and siplicity. This work is constituted by three parts: first describer theoretical base, second part is dedicate to analysis economy from 1993 and third part is focused on macroeconomical model of Czech economy. Theoretical part presents main parts of national economy (e.g. Gross domestic product, Unemployment, Inflation, Balance of Payment, etc.) including detailed structure of these key indicators. Such a detailed description was importent because of recent modelling and was signiicant for better choice of variable. In second part of this work we analyse economic development from 1993 and we focused on main causes of change. Main goal of this part was exploring of global progress and better understanding to all indicators in the small-open-economy. In final part of dissertation are created several models which are based on historical data and predict progress for nearest future. Because fact that source data are mainly till 2010 we were able to test our models on real data of year 2011. In spite of economical progres in last few years we can say that large majority of results from our models are in compliance with our expectation. However in some cases ware results diferent, we identifed key resons for these results.
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