National Repository of Grey Literature 38 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Designing CLIL activities for teaching mathematics at upper secondary school level
Řehák, Česlav ; Moraová, Hana (advisor) ; Novotná, Gabriela (referee)
This thesis seeks to create, test and discuss the success of several activities for the second year of the upper secondary education, where the target language is English with 20 % of all lessons taught with the use of CLIL. Individual activities are described from the points of view of the planned and the piloted course of the lesson, including reflection on which aspects of the activity worked and which require further adjustments in order to improve upon the motivational and educational impact. At the end of the thesis, the reception of the activities by the students, the influence of CLIL teaching on the students' motivation and the enthusiasm with which they participated is summarised. The fulfilment of the thematic plan and most importantly the common attributes of those activities that can be viewed as successful in respect to piloting are discussed. These notions are put into context of the generally accepted principles of CLIL teaching, which are described in the theoretical part. The need to create new teaching materials and develop new activities for upper secondary mathematics are discussed and defended in the theoretical part.
Global epidemiology of Staphylococcus aureus circulating in healthcare system and community
Mrázková, Pavlína ; Tkadlec, Jan (advisor) ; Balíková Novotná, Gabriela (referee)
Staphylococcus aureus is an important human pathogen responsible for a wide range of infections occurring among patients in the healthcare setting and in the community. Much attention is focused on methicillin-resistant strains (MRSA), but methicillin-sensitive strains (MSSA) also remain the main cause of infections. The epidemiology of S. aureus, especially among MRSA strains, has shown a rapid development in recent years, characterized by the appearance of epidemic lines that dominate in certain geographical areas. Molecular typing techniques have made it possible to identify the main successful lineages of MRSA and MSSA and to track their spread and report the prevalence of certain clones in the area at the same time. To understand the evolution and spread of S. aureus, as well as to deploy effective preventive measures against the spread of this dangerous pathogen, the knowledge of the S. aureus strains epidemiology is absolutely necessary. As part of the bachelor's thesis, a recent overview of the methods used for the clonal analysis of Staphylococcus aureus will first be presented. Furthermore, a literature search will be conducted with the aim of creating an overview of the dominant strains of S. aureus and MRSA circulating in the hospitals and in the community with an emphasis on the...
Cellular factors influencing the antibiotic resistance by ABC-F proteins
Kýr, Jan ; Balíková Novotná, Gabriela (advisor) ; Dolejšová, Tereza (referee)
Antibiotic resistance is one of the main problems modern medicine has to face. In order to control it, it is important first to understand the mechanisms by which resistant pathogens bypass antibiotic treatment. One of the important protein families conferring resistance to 50S binding antibiotics is the ARE ABC-F protein family. A member of this protein family is the MsrA protein, which confers resistance to 14- and 15-membered macrolides. Loss-of-function mutations in the non-essential chaperone ClpX were found to a significant ly enhance the action of the MsrA protein. This leads to the significant increase in the resistance conferred by this protein. The exact mechanism by which ClpX affects MsrA function is still unknown. In this the diploma thesis was demonstrated that chaperone protein ClpX affects the resistance to erythromycin conferred by MsrA protein, due to the interaction with an unknown essential protein, which is mediated by a functional N-terminal zinc-binding domain of the chaperone. Furthermore, it was demonstrated in this work that loss of GluTR function influence the MsrA ability to confer resistance. The results of this work will bet he basis for further reasearch, which will lead to a more detailed understanding of the mechanism of resistance conferred by these proteins and...
Analysis of solutions and popularity of tasks from the Pangea competition
Vratislav, Milan ; Kaslová, Michaela (advisor) ; Novotná, Gabriela (referee)
The submitted bachelor's thesis deals with the relationship between the mathematical focus of tasks, success rate, popularity and point evaluation of tasks from the Pangea mathematical competition. The analysis refers to tasks for the 9th grade of the Pangea competition from the final round in the 2019/2020 school year, which took place a year later, in the 2020/2021 school year, due to the coronavirus pandemic. Determining the relationships between the investigated variables is based on quantitative research on problem solvers. Task success rate data was provided by Pangea competition management. The level of popularity of the tasks was determined based on data from the questionnaire, which is filled out annually by the solvers of the tasks immediately after submitting the solution. The characteristics and main focus of the tasks were described by the author of this work. The point evaluation of the tasks is determined by the authors of the tasks. The analysis of the tasks refers to their assignment, the expected solution procedure, the expected difficulties in the solution and comments on the answer menu. The thesis defines the terms gifted student, mathematical competition and word problem, and defines the specifics and mission of the Pangea competition. The results of the work can be used when...
Glycopeptide resistance in staphylococci
Rozsypálková, Adéla ; Tkadlec, Jan (advisor) ; Balíková Novotná, Gabriela (referee)
The aim of this thesis is to describe the mechanism of the resistance to glycopeptide antibiotics in a genus Staphylococcus, especially in a species Staphylococcus aureus which is common cause of nosocomial infections resulting frequently in expensive and long-term treatment. This pathogen is dangerous due to its ability to acquire resistance to most antibiotics used in a clinical practice. The resistance of these microorganisms can develop very easily due to inappropriate treatment (administration, drug concentration, duration), which, if not detected, could ultimately results in treatment failure and the death of the patient. The vancomycin resistance of S. aureus could be divide into groups according to their values of vancomycin MIC: VSSA, VISA, hVISA and VRSA. Vancomycin intermediate resistance is associated with mutation, e.g., which affect cell wall synthesis. In contrast, VRSA is associated with the transfer of the mobile genetic element with the vanA or vanB operon from genus Enterococcus. This transmission is due to co-infection with both pathogens. Glycopeptide resistance has also been shown to be very common in coagulase-negative staphylococci (CNS), such as S. capitis, which cause infection in preterm infants. Glycopeptide resistance in CNS and intermediate resistance of S. aureus is...
Subcellular localization of resistant proteins Vga(A)LC and Msr(A) using fluorescence microscopy
Nguyen Thi Ngoc, Bich ; Balíková Novotná, Gabriela (advisor) ; Lichá, Irena (referee)
Vga(A)LC and Msr(A) are clinically significant resistant proteins in staphylococci that confer resistance to translational inhibitors. They belong to ARE ABC-F protein subfamily, which is part of ABC transporters. Unlike typical ABC transporters, ABC-F proteins do not have transmembrane domains that are responsible for the transport of substances through the membrane. Therefore, they do not have characteristic transport function but regulatory or resistance function. Their mechanism of action on the ribosome has been described only recently, where these proteins displace the antibiotic from the ribosome. However, some aspects of their function are still unclear. For example, what is the function of the Vga(A) location on a membrane that has been detected in the membrane fraction but not in the ribosomal. In this work, using fluorescence microscopy, I observed subcellular localization of the Vga(A)LC-mEos2, Vga(A)LC-GFP and Msr(A)-eqFP650 resistant fusion proteins in live cells of S. aureus under different culture conditions . It has been shown that Vga(A)LC-GFP and Msr(A)-eqFP650 occur in a foci near the membrane. Depending on ATPase activity or the presence of an antibiotic, the localization of Msr(A)-eqFP650 in the cell changes from focal to diffuse, presumably on ribosomes, suggesting a...
Antibiotic resistance conferred by members of ARE subfamily of ABC proteins
Veselá, Ludmila ; Balíková Novotná, Gabriela (advisor) ; Borčin, Kateřina (referee)
The main topic of this thesis is the ARE subfamily of ABC transporters. The importance of the proteins of this subfamily lies in the fact that they confer resistance to several classes of clinically important antibiotics: macrolides, lincosamides, streptogramines and pleuromutilines and they do it in significant pathogens, as for example Staphylococcus aureus. Compared to canonical ABC transporters, the structure of ABC proteins lacks the transmembrane domain (TMD) and so far, there where not even found an integrating transmembrane protein. Due to these facts, the mechanism of resistance conferred by these proteins remains unclear. In the thesis, both suggested hypotheses of the mechanism of how these proteins work are discussed. The first hypothesis presumes the active efflux of antibiotics out of the bacteria. The second hypothesis suggests release of antibiotic from its binding site initiated by ARE proteins, followed by its passive diffusion out of the cell. Keywords: ABC proteins, ARE proteins, resistance, MLS, Vga
Commonly Mispronounced English Words by Czech Speakers
Novotná, Gabriela ; Červinková Poesová, Kristýna (advisor) ; Bojarová, Marie (referee)
The first chapters of this thesis describe the process of collecting mispronounced words in English, first by searching through various sources including course books, didactic manuals and teachers' books; and second by attending English lessons at various schools and listening to Czech students' production of English. The collected data were classified according to the cause of observed mispronunciations. The established categories were analysed and commented from the phonetic point of view. The practical part further contains a small-scale research carried out among university students of the English language in which their reading was recorded and evaluated in relation to the examined phenomenon. Key words commonly mispronounced words, Czech native speakers, stress placement, silent letters 1
The effect of aminoacid variability on the resistance phenotype in ARE subfamily of ABC proteins
Lenart, Jakub ; Balíková Novotná, Gabriela (advisor) ; Fišer, Radovan (referee)
ARE subfamily proteins belonging to ABC transporters confers a different degree of resistance to macrolides, linkosamides and streptogramins antibiotics. Among the most clinically ARE subfamily proteins in staphylococci is Vga(A) protein lead to the award resistance to streptogtramins A. In 2006, discovered the new variant called the Vga(A)LC, which in addition to streptogramins A resistance also confers linkosamides. Vga(A) and Vga(A)LC differ in only 7 amino acids, yet confer different resistance phenotypes. In previous experiments it was found that the central role in determining substrate specificity play a 4 amino acid differences that accumulate in the section of 15 amino acids within the linker connecting the two ABC domains (positions 212, 219, 220 and 226). The combination of amino acids LGAG Vga(A) increases resistance to streptogramins A while present in combination SVTS Vga(A)LC increased resistance to linkosamides. Although in this subfamily includes a large number of resistance proteins, the mechanism of resistance has not yet been established with certainty. The aim was to create a new Vga(A) variants that contain specific combinations of amino acids for Vga(A) and Vga(A)LC protein at positions 212, 219, 220 and 226 and compared their ability to grant resistance to linkosamides. We also...

National Repository of Grey Literature : 38 records found   1 - 10nextend  jump to record:
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15 NOVOTNÁ, Gabriela
5 Novotná, Gražyna
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