National Repository of Grey Literature 131 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Optimization and validation of UHPSFC-UV methods for quality control of agomelatin and atorvastatin in drug substance and in tablets
Němcová, Zdeňka ; Nováková, Lucie (advisor) ; Khalikova, Maria (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Zdeňka Němcová Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. Title of Diploma Thesis: Optimization and validation of UHPSFC-UV methods for quality control of agomelatin and atorvastatin in drug substance and in tablets The aim of this master thesis was to develop two ultra-high performance supercritical fluid chromatography (UHPSFC) methods with PDA detection for determination of agomelatine and atorvastatin and its potential impurities. UHPSFC system Acquity UPC2 with PDA detector was used for measurement. The separation of agomelatine was performed by Torus Diol column (100 × 3.0 mm, 1.7 µm). Gradient elution was performed using CO2 with the mixture of methanol/acetonitrile (1:1) and 31.5 mM (0,1 %) ammonium hydroxide as a mobile phase. Column temperature was set at 40 řC and BPR (back pressure regulator) pressure at 2000 psi. The detection wavelength was set at 225 nm. The method was developed for measurment of API (active pharmaceutical ingredient) and subsequently for tablets. The separation of atorvastatin was performed using Acquity UPLC HSS C18 SB column (100 × 3.0 mm, 1.8 µm). Gradient elution was performed using CO2 with the mixture of methanol/ acetonitrile (2:1), 15 mM...
Comparison of two types of matrix effect evaluation for LC-MS/MS analysis
Lejnarová, Michaela ; Kočová Vlčková, Hana (advisor) ; Nováková, Lucie (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Michaela Lejnarová Supervisor: RNDr. Hana Kočová Vlčková, Ph.D. Title of Diploma Thesis: Comparison of two types of matrix effect evaluation for LC-MS/MS analysis This diploma thesis deals with a comparison of two methods of matrix effect evaluation in LC-MS/MS analysis using electrospray ionization and triple quadrupole. Specifically, it was a comparison of the method using slopes of a line of the calibration curves and the post-extraction addition method, where the main goal was to assess whether the obtained values of matrix effects correlate with each other or not. Where appropriate, to clarify the reasons for the different outcomes and define the conditions of both methods to obtain correct and accurate results. On the basis of physicochemical properties such as molecular weight, acid-base properties, and partition coefficient, a group of 28 diverse substances was selected. The actual evaluation of matrix effects was preceded by the selection of an appropriate LC method, optimization of the individual parameters of the ion source as well as the selection of appropriate SRM transition and collision energy for each substance. To evaluate the matrix effects, lyophilized serum was chosen as a...
Application of supercritical fluid chromatography in analysis of natural compounds
Lacmanová, Tereza ; Nováková, Lucie (advisor) ; Pilařová, Veronika (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Lacmanová Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. Title of Diploma Thesis: Application of supercritical fluid chromatography in analysis of natural compounds The goal of this diploma thesis is to verify the suitability of UHPSFC method with PDA and MS detection for analysis of natural products. For the analyzes, two representatives of natural compounds - the alkaloids of the family Amaryllidaceae with effect in the treatment of Alzheimer's disease and phenolic compounds with antioxidant activity - flavonoid rutin, quercetin and their metabolites were chosen. Separations were carried out on the UHPSFC system Acquity UPC2 with PDA and MS detector. The basic screening was performed on 10 stationary phases: BEH 2-EP, HSS SB C18, BEH, Torus DIOL, Torus 2-PIC, Torus DEA, CSH PFP, Torus 1-AA, Cortecs HILIC and BEH Amide. Four mobile phases were tested: 1. CO2 + MeOH, 2. CO2 + MeOH + 0.1 % NH4OH, 3. CO2 + MeOH + 10 mM ammonium formate, and 4. CO2 + MeOH + 10 mM ammonium formate + 2 % H2O. Separation conditions were as follows: flow rate 1.5 ml/min, temperature 40 řC, pressure 140 bar, gradient elution from 2 % to 40 % within 4 minutes, with two isocratic steps at the beginning and...
Development and validation of UHPLC-MS/MS method for determination of maraviroc in placental perfusion
Čapková Maxová, Eliška ; Nováková, Lucie (advisor) ; Kočová Vlčková, Hana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Eliška Čapková Maxová Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. Title of Thesis: Development and validation of UHPLC-MS/MS method for the determination of maraviroc in placental perfusions The goal of this work was to develop fast and selective method for the determination of maraviroc in human placental perfusions. The ultra-high performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) was used. BEH C18 column and gradient elution with the mobile phase A (water with 0.1% formic acid) and B (acetonitrile) at 0.35 mL/min flow-rate and 40 řC temperature were used for the separation. The mass spectrometry conditions were optimist and set up as follows: electrospray ionization in positive mode, capillary voltage 1.0 kV, cone voltage 35 V, extractor 3.0 V, RF lens 0.1 V, desolvatation gas flow 1000 L/h, temperature 450 žC, cone gas flow 100 l/h, ion source temperature 130 žC. Selected reaction monitoring (SRM) mode was used for quantitation. Liquid-liquid extraction (LLE) was chosen for sample preparation and was optimised. The best results were obtained when dichloromethane was used as the extraction agent (recovery >90%). The optimized method was fully validated in...
Návrh na doplnění územního systému ekologické stability ve vybraném území
NOVÁKOVÁ, Lucie
This bachelor thesis is focused on territorial system of ecological stability. For the sloved area are described all the individual parts of the territorial system of ecological stability with the characterictics of actual condition. New elements have been proposed, to strenghten the environmental stability.
Impacts of Selected Changes in Accounting Legislation on Reporting and Comparison of Data
NOVÁKOVÁ, Lucie
The thesis deals with the evaluation of changes in accounting legislation in relation to the informative ability of accounting. In particular, these changes are: - Canceling Reporting Item Separation Reporting alone - Changes in accounting for provisions - Accounting for activation and inventory of own activity - Categorization of entities and related obligations - Cancellation of extraordinary costs and revenues - Amendment to the regulations governing the auditor's audit of financial statements - Canceling the separate reporting of the Trade Margin item As the Czech Republic is a part of the European Union, it is impossible to ignore the effort to unify repor-ting, which is intended to improve the readability of financial statements for users from other countries. The work includes an evaluation of changes from the perspective of various authors and the professional public. Selected statements are verified on selected businesses. The ongoing small legislative adjustments do not fully meet the needs of current businesses, the concept of the new form of accounting, which is currently presented to the professional public for discussion, indicates the future direction of Czech ac-counting. An equally important part of the thesis is the analysis of the questionnaire, which seeks to gain the opinion of the professional public on the issue.
Elizabeth Bishop: The Map of Her Life and Work
Nováková, Lucie ; Veselá, Pavla (advisor) ; Quinn, Justin (referee)
The aim of the thesis is straightforward: to provide readers with a glimpse into the life of Elizabeth Bishop but not to put emphasis on biographical details. The focus lies on her two most formative relationships with her fellow poets, namely Marianne Moore and Robert Lowell. The thesis aims to present five selected poems and to read them with acknowledging the mutual influences and, at the same time, it strives to provide specific instances of such influences. The first part of the thesis is dedicated to the relationship with Marianne Moore. The two poets met during Bishop's Vassar years and their friendship lasted until Moore's death in 1972. From the teacher - mentored paradigm, their friendship evolved into an affectionate companionship. The thesis introduces their relationship while using selected letters, interviews and, to illustrate the matters more clearly, two of Bishop's poems, "The Roosters" and "Invitation to Miss Marianne Moore." The first poem captures the breaking free from Moore's direct influence and additionally serves as a link to the second part of the thesis. The later poem is used to illustrate their reconciliation and to present the milestone that marks the shift of paradigms. The second part of the thesis presents Robert Lowell, who is the already mentioned link between...
Evaluation of separation potential of stationary phases based on porous graphitic carbon
Guláš, Ondrej ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Ondrej Guláš Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. Title of Diploma Thesis: Evaluation of separation potential of stationary phases based on porous graphitic carbon. Stationary phases based on porous graphitic carbon (PGC) exhibit many interesting features. For example high mechanical resistance, stability across the entire pH range and excellent high temperature resistance. Moreover, they are significant due to their unique mechanism of retention. Despite all of these positive features and long existence on the market, their broader use in pharmaceutical analysis is still lacking. In our paper we have analyzed 11 groups of drugs, including active pharmaceutical ingredient and its potential impurities. Overall, 67 substances have been analyzed. Each separation on PGC has been optimized in order to achieve the best results. Changes in conditions of separation included testing of several mobile phases, gradient and isocratic elution and testing the effect of higher temperature. The use of gradient elution, with mobile phase consisting of acetonitrile and isopropanol in proportion 1:1 as organic modifier and 0,1% trifluoracetic acid as aqueous part has appeared as the most...
Development of UHPLC method for separation of eight structurally related derivatives of vitamin E
Pružinská, Klára ; Nováková, Lucie (advisor) ; Pilařová, Veronika (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Klára Pružinská Supervisor: Assoc. prof. PharmDr. Lucie Nováková, Ph.D. Title od the diploma thesis: Development of UHPLC method for separation of eight structurally related derivatives of vitamin E The aim of this diploma thesis was to develop UHLPC method for the separation of eight derivates of vitamin E. They include a group of tocopherols and tocotrienols. To achieve the aim ultra-high performance liquid chromatography was used. In process of optimalization the most important thing was the choice of stationary phase, consistence of mobile phase, flow speed, temperature of column and the time of separation. The Ascentis Express F5 Column (100 x 3.0 mm, 2.7 μm), Sigma-Aldrich was the best stationary phase because the separation of all eight derivatives on this column including two problematic pairs: β-T3 with γ-T3 and the pair β-T with γ-T was performed. The mobile phase was a mixture of a methanol with acetonitrile (1:1) and water in the ratio 75:25 with a flow rate 1 ml/min, isocratic elution and PDA and FLR detection. In PDA detection the wavelength was set 290 nm. In more sensitive FLR detection the emission wavelenght was 325 nm and the exciting wavelength of exciting was set on 280 nm....

National Repository of Grey Literature : 131 records found   1 - 10nextend  jump to record:
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1 Nováková, Leona
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