National Repository of Grey Literature 567 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Analysis of intermediate filament structure by chemical cross-linking
Dlabolová, Lada ; Novák, Petr (advisor) ; Šulc, Miroslav (referee)
Intermediate filament proteins create a dynamic cytoskeletal filamentous network, which due to its elastic properties, significantly contributes to the resistance of cells and tissues to mechanical stress. An important protein from the family of intermediate filaments, vimentin, is expressed mainly in cells of mesenchymal origin. Vimentin has been associated with a large number of pathophysiological conditions, and current studies consider vimentin as clinically promising target for the diagnosis, prognosis and treatment of a wide range of diseases from cancer to infectious and inflammatory diseases. Although in terms of structural characterization, vimentin belongs to one of the most studied proteins from the family of intermediate filaments, our knowledge is currently limited to the structure of the vimentin tetramer. Vimentin is capable of self-assembly into filaments formed by homo-oligomeric ULF subunits and the assembly process involves several steps of the organization of subunits. Structural characterization of the oligomeric subunits involved in the assembly of vimentin filaments is a prerequisite for elucidating the architecture of mature filaments, which can significantly contribute to understanding and connecting the mechanisms of many diseases associated with changes in vimentin...
Studies on structure and biological functions of NKR-P1 receptors
Rozbeský, Daniel ; Novák, Petr (advisor) ; Konvalinka, Jan (referee) ; Drbal, Karel (referee)
Natural killer (NK) cells play a significant role in the detection and destruction of virally infected and tumor cells. The NKR-P1 receptors regulate NK cell function by an alternative missing-self recognition system. Although the NKR-P1 receptors were among the first surface NK receptors identified on rodent NK cells more than 20 years ago, there is still very little known about their biological function and their physiological ligands. Furthermore, no three-dimensional structure of any of the NKR-P1 family receptors has been published so far. To understand the functional architecture of mouse NKR-P1 receptors, we developed a simple and efficient protocol providing large amounts of pure soluble NKR-P1 proteins. The crystal structure of mouse NKR-P1A, determined at 1.7 A resolution, is the first structure of a representative of the NKR-P1 family. Crystal structure is formed by a compact C-type lectin-like domain and an extended loop that participates in domain swapping. A potential role of the swapped loop has been suggested in natural ligand binding by in silico studies. However, chemical cross-linking and H/D exchange in combination with high resolution mass spectrometry revealed this loop in close proximity to the compact core in solution. The discrepancy between the crystal and solution structure...
Powder Metallurgy of Hybrid Materials for Advanced Applications
Kozlík, Jiří ; Stráský, Josef (advisor) ; Kim, Hyoung Seop (referee) ; Novák, Pavel (referee)
Powder Metallurgy of Hybrid Materials for Advanced Applications The field-assisted sintering technology (FAST) is a versatile powder consolidation method, which uses electrical current for rapid heating of the sintered material. It is routinely used for sintering of various metallic materials, but mostly with preal- loyed powders as a feedstock. The aim of the thesis is to explore the capabilities of FAST for sintering of composite materials and for using blended elemental powders as a raw material in following areas: 1. Manufacturing of architectured composites 2. Consolidation and ageing in a single processing run (in-situ ageing) 3. Rapid alloy prototyping using blended elemental powders 4. High-throughput experimental investigations of multicomponent phase dia- grams Al6061 + Ti-6Al-4V architectured composite was produced, but issues with the pow- der penetration into the lattice have arisen due to the small lattice dimensions. Me- chanical properties could have been assessed by a three-point bending only. The composite has shown improved strength over the plain, unannealed Al6061 matrix, but a premature fracture when the Al matrix was aged properly. Using FAST was found to be inconvenient for architectured composite manufacturing. A long, fully dense rod was manufactured from the Ti-5Al-5V-5Mo-3Cr...
Interpretation of the drawing of an enchanted family in the context of social work with foster families
Gazdová, Marie ; Novák, Petr (advisor) ; Drahoňovský, Jan (referee)
The bachelor's thesis will be focused on the drawing of the "enchanted family". In the theoretical part, I deal with concepts related to the family. I also deal with surrogate family care, its forms, the perception of family care, etc. The theoretical text also contains an approach to the topic of children's drawing, especially its developmental stages, family drawings and drawings of the "enchanted family." The practical part is focused on drawing an "enchanted family" with children from foster families and talking to them. With the drawing of the enchanted family, it is essential that the child can express his view of the individual members of the family by bewitching them into an animal. Drawing in my research part is used to get to know the child's personality, especially his relationships between family members and his overall experience while drawing. I also decided on child development and substitute family care. Keywords Family, Foster Care, Child, Child Drawing, Family Drawing, Enchanted Family Drawing
Motivation and psychosocial needs of workers of the social and legal protection of children
Marková, Lucie ; Novák, Petr (advisor) ; Nová, Monika (referee)
This diploma thesis deals with social workers who work in the department of social and legal protection of children. The aim of the work was to characterize the motivation factors and psychosocial needs for the performance of the work of OSPOD social workers in Strakonice. My diploma thesis was divided into a theoretical part and a practical part. In the theoretical part of my diploma thesis, I described the basic concepts related to the concept of social-legal protection of children, including historical development, legislative framework, and I described the functioning system of social-legal protection of children in the Czech Republic. Furthermore, in this section I described terms such as the Code of Ethics for social workers, motivation, psychosocial needs, psychohygiene and burnout syndrome. In the practical part, the research strategy of qualitative research was used and the method of questioning and the technique of data collection in the form of a structured interview were chosen. Communication partners were employees of the Municipal Office in Strakonice, who work as social workers in the Department of Social and Legal Protection of Children. For the research in the practical part, the goal of the thesis, the main research question and three sub- research questions, which lead to the...
GRAIL and LOLA Satellite Data Resolve the Long-Lasting Convergence/Divergence Problem for the Analytical Downward Continuation of the External Spherical Harmonic Expansions
Šprlák, Michal ; Han, Shin-Chan ; Pitoňák, Martin ; Novák, Pavel
Spherical harmonic expansions are routinely used to represent the gravitational potential and its higherorder spatial derivatives in global geodetic, geophysical, and planetary science applications. The convergence domain of external spherical harmonic expansions is the space outside the minimum Brillouin sphere (the smallest sphere containing all masses of the planetary body). Nevertheless, these expansions are commonly employed inside this bounding surface without any corrections. Justification of this procedure has been debated for several decades, but conclusions among scholars are indefinite and even contradictory. In this contribution, we examine the use of external spherical harmonic expansions for the gravitational field modelling inside the minimum Brillouin sphere. We employ the most recent lunar topographic LOLA (Lunar Orbiter Laser Altimeter) products and the measurements of the lunar gravitational field by the GRAIL (Gravity Recovery and Interior Laboratory) satellite mission. We analyse selected 39 http://dx.doi.org/10.13164/seminargnss.2023.38 quantities calculated from the most recent GRAIL-derived gravitational field models and forwardmodelled (topography-inferred) quantities synthesised by internal/external spherical harmonic expansions. The comparison is performed in the spectral domain (in terms of degree variances depending on the spherical harmonic degree) and in the spatial domain (in terms of spatial maps). To our knowledge, GRAIL is the first gravitational sensor ever, which helped to resolve the long-lasting convergence/divergence problem for the analytical downward continuation of the external spherical harmonic expansions, see [1].
Validation of Satellite Gravitational Gradients Grids by Spectral Combination Method and GNSS/Levelling Data Over Norway, Czechia and Slovakia
Pitoňák, Martin ; Šprlák, Michal ; Ophaugh, Vegard ; Omang, Ove C. D. ; Novák, Pavel
The launch of gravity-dedicated satellite missions at the beginning of the new millennium led to an accuracy improvement of global Earth gravity field models (GGMs). One of these missions was the Gravity field and steady-state Ocean Circulation Explorer (GOCE) launched in 2009. As the first ESA's Earth Explorer Mission, the satellite carried a novel instrument, a 3-D gradiometer, which allowed to measure of second-order directional derivatives of the gravitational potential (gravitational gradients) 37 http://dx.doi.org/10.13164/seminargnss.2023.36 with uniform quality and near-global coverage. The main mission goal was to determine the static Earth's gravity field with the ambitious precision of 1-2 cm in terms of geoid heights and 1 mGal in terms of gravity anomalies for a spatial resolution of 100 km (half wavelength at the equator). More than three years of outstanding measurements resulted in three levels of data products (Level 0, Level 1b and Level 2), six releases of GGMs, and several global grids of gravitational gradients. The grids, which represent a step between gravitational gradients measured directly along the GOCE orbit and those represented by GGMs, were used mainly in geophysical applications. In this contribution, we validate the official Level 2 product GRD SPW 2 using height anomalies over two test areas in central and northern Europe (Czechia/Slovakia and Norway). A mathematical model based on the least-squares spectral weighting is employed with corresponding spectral weights estimated to validate gravitational gradient grids. This model continues gravitational gradients from the mean orbital altitude of GOCE down to the irregular Earth's surface (not to a sphere) and transforms them to height anomalies in one computational step. Analytical downward continuation errors of the model are estimated using a closed-loop test. Before comparing, height anomalies estimated from gravitational gradients with their reference values derived from GNSS/levelling over the two test areas, the gravitational gradients and reference data are corrected for all systematic effects, such as the tide system conversion. Moreover, the high-frequency part of the gravitational signal is estimated and subtracted from reference data as it is attenuated in the gravitational gradients measured by GOCE. A relative improvement between the release 6 and release 2 gradient grids reaches 48% in terms of height anomalies in Czechia/Slovakia. The relative improvement in Norway is even more significant and reaches 55%. Release 6 of the official Level 2 product GRD SPW 2 gained absolute accuracy with the standard deviation of 9.1 cm over Czechia/Slovakia and 9.6 cm over Norway.
Far Zone Effects for Integral Transformations: Theory and Implementation
Trnka, Petr ; Belinger, Jiří ; Šprlák, Michal ; Pitoňák, Martin ; Novák, Pavel
Integral transformations are a useful mathematical apparatus for modelling the gravitational field and require the formulation of integral estimates including error propagation. For classical integral transformations, this issue has already been studied, but the formulation for all available gravitational observables has not been studied yet. The assumption of integral transformations is global data coverage. In practice, however, data availability is limited, so we divide the global integration into the effects of the near and far zones. The computation of distant zones is a non-negligible systematic effect requiring an accurate calculation. The theory is implemented in the form of a precise software. In this paper, we present the basic theory for the evaluation of the far zones. We also investigate properties of integral kernels and truncation error coefficients. In the numerical experiments, we compare calculation of the far zones by numerical integration with truncated spherical harmonic series. One of the outputs of this contribution is a software library for computation of the far zones for integral transformations mutually relating all quantities up to the third derivatives of the gravitational potential.

National Repository of Grey Literature : 567 records found   1 - 10nextend  jump to record:
See also: similar author names
76 NOVÁK, Pavel
101 NOVÁK, Petr
12 Novák, P.
3 Novák, Patrik
76 Novák, Pavel
76 Novák, Pavel
101 Novák, Petr
3 Novák, Přemek
2 Novák, Přemysl
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