National Repository of Grey Literature 11 records found  1 - 10next  jump to record: Search took 0.01 seconds. 
Whole genome synthesis: methodology and applications
Kučera, Vítězslav ; Hlouchová, Klára (advisor) ; Nedvědová, Jana (referee)
Genomes contain the information needed to support life and viruses. Understanding this information is no small task but an essential one for the understanding of life and its evolution. Genome sequencing can provide useful information about the meaning of genetic information, although it is now clear that sequencing alone cannot provide all the answers. For deeper comprehension of life's intricacies, synthesis of genomic DNA will extend our current knowledge and offer a platform for further applications. The immense growth in the methodology of DNA synthesis in the last decades facilitated the construction of the first cellular genome in 2008. Continuous advances in genome synthesis and engineering are the driving forces of synthetic biology. The history, current state and future applications of the whole genome synthesis are presented in this thesis. Key words: DNA assembly, synthetic biology, minimal cell, genome editing, genome transplantation, DNA synthesis, top-down approach, bottom-up approach
The Business Plan for Establishment of Family Pension in Town Tři Studně
Nedvědová, Jana ; Mráček, Pavel (referee) ; Milichovský, František (advisor)
This Master's thesis deals with the processing of a business plan for establishing a family guesthouse in Vysočina region in the village Tři Studně. The theoretical basis of the thesis includes development and growth of the company, company strategy, external and internal environment analysis, customer analysis and SWOT analysis. The practical part analyzes the current situation, the description of the pension and its surroundings. The design part calculates the cost of the project, predicts sales and average cost of accommodation.
Analysis of Histone Deacetylase 6/Kinesin Interactions
Nedvědová, Jana ; Bařinka, Cyril (advisor) ; Pavlíček, Jiří (referee)
Intracellular transport is provided by two major types of molecular motors kinesins and cytoplasmic dynein. Kinesin-1 is a molecular motor that transports molecules and organelles along microtubule tracks anterogradely. Specific protein-protein interactions are required to activate kinesin-1 as the free kinesin exist in an autoinhibited state. The activation of kinesin-1 induces its conformational change, enables microtubule binding and ATP hydrolysis necessary for the directional cargo transport. HDAC6 is a multifunctional protein composed of several domains. It plays an important role in many microtubule dependent processes as HDAC6 is a major tubulin deacetylase. It has been shown that HDAC6 manipulation (inhibition/genetic ablation) affects transport along microtubules but the exact mechanisms are unknown. The effect can be caused either by deacetylation microtubules or direct interaction with molecular motors. This thesis is focused on characterization of interactions between kinesin-1 and HDAC6 that have not been described so far. To this end, we expressed and purified various constructs of kinesin-1 and HDAC6 and tested their interactions by microscale thermophoresis (MST) and hydrogen deuterium exchange (HDX) to determine affinity and interaction sites, respectively. MST data revealed that...
Phylogenetic and ecological impact to the microstructures of mollusc shells
Nedvědová, Jana ; Juřičková, Lucie (advisor) ; Simon, Ondřej (referee)
This bachelor thesis summarizes the available information on microstructures of mollusc shells. The aim was to provide an overview of the different types of microstructures for specific specimens from different environments, as well as to obtain as much information as possible about the ecology of these animals. The first part summarizes the basic information on the shell formation. The following chapter summarizes known microstructure types of mollusc shells. Microstructures of molluscs living in terrestric, freshwater and marine environment are discussed. The influence of temperature changes on the shell microstructure of V. viviparus, the occurrence of vaterit within C. fluminea shells, the extreme environment of hydrothermal vents and cold seeps, as well as extreme temperatures on rocky shores, have been mentioned in more detail. The microstructures of related molluscs are compared with regard to ecology to determine whether the occurrence of microstructures in a particular group is given by the phylogeny of these animals, or if the changes in microstructures can be affected by different ecology. Key words: microstructures, molluscs shells, impact of phylogeny, impact of ecology, calcium carbonate, Mollusca, gastropods, bivalves
The effect of Ca2+ ion on the enzymatic activity of human glutamate carboxypeptidase II
Nedvědová, Jana ; Bařinka, Cyril (advisor) ; Hlouchová, Klára (referee)
Human glutamate carboxypeptidase II (GCPII, EC 3.4.17.24) is a homodimeric membrane glycoprotein. GCPII has been studied as a marker of prostate carcinoma and a therapeutic target of neurodegenerative disorders. The extracellular region of the protein is composed of three domains, apical, protease and C-terminal. There are two zinc ions in the active site that are essential for the enzymatic activity. A calcium ion is located between apical and protease domains near the dimeric interface approximately 20 Å away from the active site. Consequently, the Ca2+ ion in unlikely to participate in substrate hydrolysis. The aim of this thesis is to elucidate the function of Ca2+ in GCPII using a combination of molecular-biological, biochemical and biophysical approaches. To this end we prepared series of GCPII variants with mutations in calcium-coordinating amino acids. The mutant constructs were expressed in insect S2 cells and purified by combination of affinity and size exclusion chromatography. Enzymatic activity and thermostability of the mutants were decreased significantly. Furthermore, mutated proteins were aggregation prone and formed a monomeric GCPII species. Our results thus show that Ca2+ ion plays an essential role in proper GCPII folding as well as the formation of a homodimer molecule that is...
Evaluation of the Financial Situation of a Company and Proposals for Improvement
Nedvědová, Jana ; Krejčí Reková, Eliška (referee) ; Sághy Estélyi, Kristína (advisor)
This bachelor thesis evaluates the financial situation of the company IMOS Brno, a.s. between the years 2011-2015 and the formulation of proposals for its improvement. At first are summarizes the theoretical knowledge of financial analysis that will be used to develop in the practical part. In the next part are captured the basic information about the company and analyzes the current status of the selected company. Analysis of the company is then used in the last part for the creation of proposals to improve the current financial situation.
Level of prevention tumors in breasts in the Strakonice region
NEDVĚDOVÁ, Jana
The number of women newly diagnosed with breast cancer is about seven thousand annually, which means slightly increased incidence. The causes of this disease are not quite clear. What is known, however, are the risk factors. Primary prevention focuses mainly on the suppression or elimination of risk factors. Secondary prevention aims at diagnosing the cancer at an early clinical stage, which enables more efficient treatment. The first preventive mammographic screening was introduced in the Czech Republic in 2002. It is a preventive examination of mammary glands by means of Mammogram, which is covered by health insurance for all women over the age of 45 once in two years. The survey on the level of prevention of breast cancer in the region of Strakonice was conducted by means of a questionnaire which was distributed among the patients of general practitioners in the selected towns and districts. The objective of the paper was to find out whether the women in the Strakonice region are informed of the risk factors of the disease. Another topic addressed was the breast self-examination. Women under 45 depend purely on it, as they are not entitled to free screening covered by health insurance. Women have been found to carry out breast self-examination quite rarely, and therefore, it would be beneficial to familiarize these women with the self-examination technique via general practitioners, gynecologists, or through the media. Self-examination should be commonly conducted by every woman over 20. Another goal of the paper was to find out what experience women had with imaging screening technologies in mammography, especially whether women over 45 undergo regular preventive mammographic screening. Also, how accessible such an examination is in the Strakonice region. At present, there are only three mammography centers in the South Bohemia region, none of which is in Strakonice. It was found that 82% of respondents over 45 take part in the screening program, and 78% of them would appreciate if a mammography center were established in Strakonice.
Income statement informative according to the Czech accounting legislation and IFRS
Nedvědová, Jana ; Procházka, David (advisor) ; Černý, Václav (referee)
The content of the thesis is an income statement informative. It deals with its structural elements, the structure itself and its various concepts within the global harmonization. Especially it focuses on defining of the same and different views on the income statement that occur between the Czech accounting legislation and IFRS. The analytic part of the thesis transforms the income statement generated under the Czech law to the income statement fulfilling the IFRS requirements and the analysis of the both reports. One part of the thesis is aimed at inter-company comparison of companies with the same type of business. It includes the horizontal and vertical analysis, including benchmarking profitability and others.
THE PREVENTION OF SENIORS´ INJURIES
NEDVĚDOVÁ, Jana
The prevention of seniors´injuries The injuries of people at seniors age have become one of the serious issues of today´s medicine, presenting not only the health problem but also the social one. The most seniors´injuries associates with falls and slips. The second main cause for injuries are the traffic accidents, the next one following upon are the accidents, which happen often at home during the casual activities. The intoxication at the old age is not rare either. The prevention of the falls and potential injuries is based on reduction of hazards of accident occurrence. The prevention, which leads to continuing reduction of the accident rate at seniors age corresponds with the goal no. 5 that is stated in the World Hospital Organization {\clqq}Health 21`` Document. The chosen exploratory method was the quantitative sociologic research based on on-the-spot requiries by the use of question form. The research sample of population consisted from seniors from 65 years old up (130 correspondents) chosen randomly in the region of České Budějovice within the year 2005. The data were all compiled in the Organization For Injuries Preventions in South Bohemia software program. Seniors faced the health hazard and injuries mostly at homes, which are mostly caused by restriction in movements. The most casual ones are the falls. The protective instruments and equipment, which might reduce the injury risks (for example non-barrier entries, skidding devices, bars and so on) still are not the standards for seniors today. The knowledge and understanding the emergancy phone numbers is not sufficient either. The possibilities of prevention for seniors are various, but have not been much exploited yet. It seems the seniors should be firstly adressed by public health employees, which they are in casual contact with and they usually rely on. The topic of prevention of injuries should be also more discussed at education programs destined for seniors.
Konstrukce neoznačených mutantů v genech kódujících Ser/Thr proteinkinasy a fosfoproteinfosfatasu Pseudomonas aeruginosa a analýza jejich vlastností
Nedvědová, Jana ; Lněnička, Petr ; Hercík, Kamil ; Branny, Pavel
To investigate the importance of individual protein kinases and phosphatases in P. aeruginosa physiology we prepared the single and double mutants in the coresponding genes. Single mutants in protein kinase genes stk1, ppkB and protein phosphatase gene stp1 were prepared by the method of gene replacement with subsequent excision of antibiotic cassettes using Flp-FRT recombination system. Two-dimensional gel electrophoresis was used to analyze the patterns of phosphoproteins obtained in in vitro protein kinase assays with the cell-free lysates of wild type as well as all mutant strains.

National Repository of Grey Literature : 11 records found   1 - 10next  jump to record:
See also: similar author names
10 NEDVĚDOVÁ, Jana
1 Nedvědová, Jitka
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