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National Repository of Grey Literature

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Comparison of devices and microscopical assesment differential count of leukocytes NETREFOVÁ, Veronika This thesis deals with methods of determination of differential count of leukocytes and their positives and negatives. No wonder that nowadays modern haematology analyzers have been used for determination of differential count of leukocytes due to technical progress, however manual assesment by microscope keep still being a basic element of good work in a haematology laboratory. In the practical part I will describe single work procedures and these methods will be then compared according to the observed results. The Coulter LH 750 analyzer by Beckman Coulter Inc. based on the principle of electric impedance has been used in Nemocnice České Budějovice a.s. for automatic assesment of differential count of leukocytes. In case of the microscopic assesment a blood film is made which is then stained by Pappenheim method with May - Grünwald and Giemsa - Romanovsky colours. Then I will try to suggest an optimization for current investigation of the complete blood picture. Detailed record

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