National Repository of Grey Literature 15 records found  1 - 10next  jump to record: Search took 0.01 seconds. 
Study of bioactive secondary metabolites produced by fungi of genus Geosmithia using UPLC-DAD-TOF-MS
Musil, Karel ; Bosáková, Zuzana (advisor) ; Tesařová, Eva (referee)
(EN) This work is dealing with study of fungal strain RJ0258 (Geosmithia sp.9) which was previously found to be the producer of secondary metabolites with antimicrobial activity. Production of secondary metabolites is significantly dependent on the conditions of cultivation and the subsequent gaining of these metabolites depends on the extraction method as well. This thesis involves the optimization of the cultivation conditions for fungal culture to ensure maximum production of substances with antimicrobial activity, the subsequent treatment of the fermentation medium, the extraction procedure focused on biologically active substances and finally the chromatographic separation. The most suitable design of the fermentation broth was chosen based on the tests - Malt extract agar medium (MEA - 100 ml, malt extract - 2 g, glucose - 2 g, pepton 0,1 g, pH adjusted to 5-6). Moreover, it was found out, that the optimal time of cultivation is 11 days, because, according to the test of biological activity, there is the highest production of secondary metabolites with antimicrobial activity. Following cultivation, the fermentation medium was extracted liquid-liquid extraction employing solution of 5% acetic acid in ethyl acetate. After evaporation and dissolution of the extract the obtained extract was...
Porovnání metod pro odhad omezených veličin s aplikací na ekonomická data
Musil, Karel ; Pavelková, Lenka (advisor) ; Hlávka, Zdeněk (referee)
The thesis introduces an overview of techniques for filtering of unobserved variables using a state-space representation of a model and state inequality constraints. It is mainly aimed at a derivation of the linear Kalman filter, its extension into a form of a non-linear filter and imposing state constraints. The state uniform model with noise bounds and the sequential importance sampling, as a method of particle filters using Monte Carlo simulations, are described as alternative methods. These three methods are applied on a simple semi-structural model for a monetary policy analysis. The filtration is based on Czech macroeconomic data and reflects an imposed non-negative state constraint on the interest rate. Results of the algorithms are compared and discussed.
Seasonality and periodicity in time series
Musil, Karel ; Jonáš, Petr (advisor) ; Cipra, Tomáš (referee)
This work deals with periodicity and seasonality in time series. After a time series periodicity topic is introduced, a seasonal component of time series and a seasonal adjustment is presented. Then basic approaches, used in current practice, are introduced. These are classic model approach, Box-Jenkins methodology, and spectral analysis. The described seasonal adjustment techniques are applied to the time series of the Czech import, export, and foreign trade balance. A brief description of potential problems, which are connected to the seasonal adjustment and are common in practice, is a part of the example as well.
Development of HPLC-DAD-MS/MS method for determination of selected toxins in Amanita phalloides.
Musil, Karel ; Bosáková, Zuzana (advisor) ; Pacáková, Věra (referee)
(EN) This work has been focused on the development and optimization of analytical methodology, using high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography with diode array and tandem mass spectrometric detection (HPLC-DAD-MS/MS) for the determination of amatoxins (α- and β-amanitin) and phalotoxins (phallacidin and phalloidin) in the crude extract from cap of Amanita phalloides with the option to later use for clinical purposes. In order to guarantee the reliability of the analytical results the influence of various parameters on the quality of the separation and the determination of toxins was studied. The developed HPLC-DAD method achieves good linearity in the concentrations range 1 - 100 µg/ml, with correlation coefficients higher than 0,997. Limits of detection (LOD) and quantitation (LOQ) were calculated for all the studied toxins with following values: for α-amanitin 0,90 µg/ml (LOD); 2,99 µg/ml (LOQ), β-amanitin 1,07 µg/ml (LOD); 3,56 µg/ml (LOQ), phallacidin 2,17 µg/ml (LOD); 7,26 µg/ml (LOQ) and phalloidin 0,79 µg/ml (LOD); 2,64 µg/ml (LOQ). Key words: Amanita phalloides,-amanitin,-amanitin, phallacidin, phalloidin, solid-liquid extraction, HPLC-DAD, HPLC-DAD-MS/MS .
Methods for Constrained State Estimation: Comparison and Application to Zero-Bound Interest Rate Problem
Musil, Karel ; Hlávka, Zdeněk (referee)
The thesis introduces an overview of techniques for filtering of unobserved variables using a state-space representation of a model and state inequality constraints. It is mainly aimed at a derivation of the linear Kalman filter and imposing state constraints. The state uniform model with noise bounds and the sequential importance sampling, as a method of particle filters using Monte Carlo simulations, are described as alternative methods. These three methods are applied on a simple semi-structural model for a monetary policy analysis. The filtration is based on Czech macroeconomic data and reflects an imposed time-varying non-negative state constraint on the nominal interest rate. Results of the algorithms are compared and discussed. Powered by TCPDF (www.tcpdf.org)
A Macroeconomic Forecasting Model of the fixed exchange rate regime for the oil-rich Kazakh economy
Hlédik, Tibor ; Musil, Karel ; Ryšánek, Jakub ; Tonner, Jaromír
This paper presents a semi-structural quarterly projection open-economy model for analyzing monetary policy transmission and macroeconomic developments in Kazakhstan during the period of the fixed exchange rate regime. The model captures key stylized facts of the Kazakh economy, especially the important role of oil prices in influencing the economic cycle in Kazakhstan. The application of the model to observed data provides a reasonable interpretation of Kazakh economic history, including the global crisis, through to late 2015, when the National Bank of Kazakhstan introduced a managed float. The dynamic properties of the model are analyzed using impulse response functions for selected country-specific shocks. The model’s shock decomposition and in-sample forecasting properties presented in the paper suggest that the model was an applicable tool for monetary policy analysis and practical forecasting at the National Bank of Kazakhstan. In a general sense, the model can be considered an example of a quarterly projection model for oil-rich countries with a fixed exchange rate.
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Development of HPLC-DAD-MS/MS method for determination of selected toxins in Amanita phalloides.
Musil, Karel ; Bosáková, Zuzana (advisor) ; Pacáková, Věra (referee)
(EN) This work has been focused on the development and optimization of analytical methodology, using high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography with diode array and tandem mass spectrometric detection (HPLC-DAD-MS/MS) for the determination of amatoxins (α- and β-amanitin) and phalotoxins (phallacidin and phalloidin) in the crude extract from cap of Amanita phalloides with the option to later use for clinical purposes. In order to guarantee the reliability of the analytical results the influence of various parameters on the quality of the separation and the determination of toxins was studied. The developed HPLC-DAD method achieves good linearity in the concentrations range 1 - 100 µg/ml, with correlation coefficients higher than 0,997. Limits of detection (LOD) and quantitation (LOQ) were calculated for all the studied toxins with following values: for α-amanitin 0,90 µg/ml (LOD); 2,99 µg/ml (LOQ), β-amanitin 1,07 µg/ml (LOD); 3,56 µg/ml (LOQ), phallacidin 2,17 µg/ml (LOD); 7,26 µg/ml (LOQ) and phalloidin 0,79 µg/ml (LOD); 2,64 µg/ml (LOQ). Key words: Amanita phalloides,-amanitin,-amanitin, phallacidin, phalloidin, solid-liquid extraction, HPLC-DAD, HPLC-DAD-MS/MS .
Study of bioactive secondary metabolites produced by fungi of genus Geosmithia using UPLC-DAD-TOF-MS
Musil, Karel ; Bosáková, Zuzana (advisor) ; Tesařová, Eva (referee)
(EN) This work is dealing with study of fungal strain RJ0258 (Geosmithia sp.9) which was previously found to be the producer of secondary metabolites with antimicrobial activity. Production of secondary metabolites is significantly dependent on the conditions of cultivation and the subsequent gaining of these metabolites depends on the extraction method as well. This thesis involves the optimization of the cultivation conditions for fungal culture to ensure maximum production of substances with antimicrobial activity, the subsequent treatment of the fermentation medium, the extraction procedure focused on biologically active substances and finally the chromatographic separation. The most suitable design of the fermentation broth was chosen based on the tests - Malt extract agar medium (MEA - 100 ml, malt extract - 2 g, glucose - 2 g, pepton 0,1 g, pH adjusted to 5-6). Moreover, it was found out, that the optimal time of cultivation is 11 days, because, according to the test of biological activity, there is the highest production of secondary metabolites with antimicrobial activity. Following cultivation, the fermentation medium was extracted liquid-liquid extraction employing solution of 5% acetic acid in ethyl acetate. After evaporation and dissolution of the extract the obtained extract was...
Porovnání metod pro odhad omezených veličin s aplikací na ekonomická data
Musil, Karel ; Pavelková, Lenka (advisor) ; Hlávka, Zdeněk (referee)
The thesis introduces an overview of techniques for filtering of unobserved variables using a state-space representation of a model and state inequality constraints. It is mainly aimed at a derivation of the linear Kalman filter, its extension into a form of a non-linear filter and imposing state constraints. The state uniform model with noise bounds and the sequential importance sampling, as a method of particle filters using Monte Carlo simulations, are described as alternative methods. These three methods are applied on a simple semi-structural model for a monetary policy analysis. The filtration is based on Czech macroeconomic data and reflects an imposed non-negative state constraint on the interest rate. Results of the algorithms are compared and discussed.
Methods for Constrained State Estimation: Comparison and Application to Zero-Bound Interest Rate Problem
Musil, Karel ; Hlávka, Zdeněk (referee)
The thesis introduces an overview of techniques for filtering of unobserved variables using a state-space representation of a model and state inequality constraints. It is mainly aimed at a derivation of the linear Kalman filter and imposing state constraints. The state uniform model with noise bounds and the sequential importance sampling, as a method of particle filters using Monte Carlo simulations, are described as alternative methods. These three methods are applied on a simple semi-structural model for a monetary policy analysis. The filtration is based on Czech macroeconomic data and reflects an imposed time-varying non-negative state constraint on the nominal interest rate. Results of the algorithms are compared and discussed. Powered by TCPDF (www.tcpdf.org)

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14 MUSIL, Karel
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