|
|
Antiprotozoal activity of natural compounds
Muchová, Michaela ; Vytlačilová, Jitka (advisor) ; Karlíčková, Jana (referee)
1 Abstract Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Author: Michaela Muchová Supervisor: RNDr. Jitka Vytlačilová, Ph.D. Title of diploma thesis: Antiprotozoal activity of natural compouds Increasing resistance to commonly used drugs in the treatment of protozoal infections has stimulated research of new drugs. Potential source of these new drugs could be found in natural substances. This thesis deals with testing of antiprotozoal activity of isoquinoline alkaloids berberine, palmatine and papaverine. The effect of individual tested compounds was observed on unicellular model organism Tetrahymena thermophila. Percentage inhibition of growth and reproduction of this protozoan was determined using 24-hour multi-generational test. Median inhibitory concentration 24hIC50 was calculated from the measured values. Papaverine showed the strongest antiprotozoal activity among testing compounds. Antiprotozoal activity decreased in the following order papaverine > palmatine > berberine. All alkaloids had stronger antiprotozoal activity than ornidazole, which was used as a standard antiprotozoal agent. Key words: antiprozoal activity, berberine, palmatine, papaverine, ornidazole, Tetrahymena thermophila
|
|
|
Antiprotozoal activity of natural compounds
Muchová, Michaela ; Vytlačilová, Jitka (advisor) ; Karlíčková, Jana (referee)
1 Abstract Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Author: Michaela Muchová Supervisor: RNDr. Jitka Vytlačilová, Ph.D. Title of diploma thesis: Antiprotozoal activity of natural compouds Increasing resistance to commonly used drugs in the treatment of protozoal infections has stimulated research of new drugs. Potential source of these new drugs could be found in natural substances. This thesis deals with testing of antiprotozoal activity of isoquinoline alkaloids berberine, palmatine and papaverine. The effect of individual tested compounds was observed on unicellular model organism Tetrahymena thermophila. Percentage inhibition of growth and reproduction of this protozoan was determined using 24-hour multi-generational test. Median inhibitory concentration 24hIC50 was calculated from the measured values. Papaverine showed the strongest antiprotozoal activity among testing compounds. Antiprotozoal activity decreased in the following order papaverine > palmatine > berberine. All alkaloids had stronger antiprotozoal activity than ornidazole, which was used as a standard antiprotozoal agent. Key words: antiprozoal activity, berberine, palmatine, papaverine, ornidazole, Tetrahymena thermophila
|
guest ::
RSS feed.