National Repository of Grey Literature 176 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Monitoring the transfer of albendazole from the sheep faeces to fodder by LC-MS
Sochová, Andrea ; Skálová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Andrea Sochová Supervisor: prof. RNDr. Lenka Skálová, Ph. D. Title of diploma thesis: Monitoring of albendazole transfer from ovine faeces to fodder plants by LC-MS In general, drugs might be a significant source of pollution in the environment especially drugs with high persistence. Albendazole (ABZ) belongs to a benzimidazole group of anthelmintic drugs. These drugs are regularly and frequently used to limit and treat parasitic infections in animals. ABZ enters the environment via animal excrements and it can have negative effects on non-target organisms. Laboratory experiments have shown that the plants can uptake and even biotransform the ABZ, however, it is not known whether these phenomena occur in the real field conditions. The present study monitors the transfer of ABZ and its transformation products (TPs) from the faeces of treated sheep to common fodder plants such as Medicago sativa and Trifolium pratense. We wanted to know whether the possibility of transferring these compounds from excrement into the soil and from soil to plants exits in real field conditions. Our study successfully revealed the occurrence of ABZ TPs (ABZ-SO and ABZ-SO2) in both fodder plants. The highest...
Development and validation of UHPLC method for determination of meloxicam in dissolution medium
Švaňová, Petra ; Matysová, Ludmila (advisor) ; Sklenářová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Petra Švaňová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Development and validation of a UHPLC method for determination of meloxicam in a dissolution medium This diploma thesis covers the development and subsequent validation of a UHPLC method for determination of meloxicam in a dissolution medium. The columm LiChrospher®, 5 µm RP-18 100 Å (125x4mm) was used for method development. As mobile phase were used methanol, isopropyl alcohol and phosphate buffer (50 mmol/l) in a 51:9:40 ratio, while the aqueous phase pH was adjusted to value 6.8. Flow rate was set to 1.0 ml/min, column temperature was 30řC and injection volume was set to 20 µl. Analytes were detected on the wavelength of 363 nm. The results of the partial validation showed that it is necessary to further optimise the method in order to provide correct results even in the dissolution medium after dissolution.
Optimization of a new analytical system for selected biochemical and immunochemical methods
Dvořáková, Kristýna ; Skálová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Kristýna Dvořáková Thesis supervizor: prof. RNDr. Lenka Skálová, Ph.D. Consultant: Mgr. Martina Hrdinová Title of diploma thesis: Optimization of a new analytical system for selected biochemical and immunochemical methods Specific requirements for validation and verification of analytical methods according to the ČSN EN ISO 15189: 2013 standard must be met by every medical laboratory. The aim of the work was to meet the requirements of verification in the introduction of a new analytical system Cobas 8000 and also to compare the results with those obtained using older analyzer Cobas 6000 in the laboratory of clinical biochemistry. For the analysis of biochemical and immunochemical methods, we used diagnostic kits from the same supplier Roche s.r.o. We determined the selected clinic-biochemical markers by the basic methods using spectrophotometry, immunoturbidimetry and electrochemiluminiscence. We used certified calibration and control material in the process of method verification. The mixed patient serum was used for the evaluation of repeatability and comparability of the methods. We evaluated the verification and repeatability of methods using Microsoft Excel software. We compared the...
Optimization of a new analytical system for selected biochemical and immunochemical methods
Dvořáková, Kristýna ; Skálová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Kristýna Dvořáková Thesis supervizor: prof. RNDr. Lenka Skálová, Ph.D. Consultant: Mgr. Martina Hrdinová Title of diploma thesis: Optimization of a new analytical system for selected biochemical and immunochemical methods Specific requirements for validation and verification of analytical methods according to the ČSN EN ISO 15189: 2013 standard must be met by every medical laboratory. The aim of the work was to meet the requirements of verification in the introduction of a new analytical system Cobas 8000 and also to compare the results with those obtained using older analyzer Cobas 6000 in the laboratory of clinical biochemistry. For the analysis of biochemical and immunochemical methods, we used diagnostic kits from the same supplier Roche s.r.o. We determined the selected clinic-biochemical markers by the basic methods using spectrophotometry, immunoturbidimetry and electrochemiluminiscence. We used certified calibration and control material in the process of method verification. The mixed patient serum was used for the evaluation of repeatability and comparability of the methods. We evaluated the verification and repeatability of methods using Microsoft Excel software. We compared the...
The effect of chitosan as excipient on dissolution of meloxicam
Machová, Barbora ; Matysová, Ludmila (advisor) ; Sklenářová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Barbora Machová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Consultant: Mgr. Jana Brokešová Title of the diploma thesis: The effect of chitosan as excipient on dissolution of meloxicam. The main objective of this diploma thesis was to develop a suitable dissolution method for evaluating the release of meloxicam from capsules and subsequently determining the effect of chitosan as excipient on the dissolution of this drug. The influence of preparation method of mixture in the capsules was also monitored. The mixture consisted of combination meloxicam with chitosan (MX-CH) in a ratio of 1:8, which was prepared by mixing in a Turbula 3D instrument, or by grinding using a planetary ball mill for 5 or 30 minutes. The dissolution was performed in a dissolution device with a paddle. The phosphate buffer with a pH of 6,8 was selected as a medium. Four formulations were tested including capsules filled with drug only and capsules filled with the mentioned mixture (MX-CH). Samples were gradually collected at time intervals and then measured with UV/VIS spectrophotometer. Based on the measured and calculated values, it is clear, that the excipient chitosan helped to better and faster release of...
Development and validation of UHPLC-MS/MS method for determination of urea in exhalted breath condensate and its application in clinical research
Vrbová, Lenka ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Lenka Vrbová Supervisor: assoc. prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Consultant: PharmDr. Kateřina Matoušová, Ph.D. Title of the diploma thesis: Development and validation of UHPLC-MS/MS method for determination of urea in exhaled breath condensate and its application in clinical research Key words: urea, exhaled breath condensate, UHPLC-MS/MS Exhaled breath condensate analysis is a simple non-invasive method of measuring biomarkers not only from the respiratory tract. For the proper clinical interpretation of the measured data it is necessary to find the dilution biomarker due to dilution of target analytes with condensed water vapour. Urea seems to be the molecule of choice. The aim of this diploma thesis was to develop a fast, simple and sensitive analytical method for determining urea concentration levels in exhaled breath condensate for clinical research. Ultra-high performance liquid chromatography with mass spectrometry detection was chosen for urea analysis. Kinetex HILIC 100 A column, 1.7 µm particle size, 100 × 2.10 mm (Phenomenex, USA) was used for the separation. The mobile phase was composed of acetonitrile, methanol and water in a ratio of 81 : 9 : 10, the organic part was...
Development and validation of HPLC method for the determination of nicotine in spray and in e-cigarette cartridges
Maťátková, Marcela ; Chocholoušová Havlíková, Lucie (advisor) ; Matysová, Ludmila (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Marcela Maťátková Supervisor: PharmDr. Lucie Havlíková, Ph.D. Title of Diploma Thesis: Development and validation of HPLC method for the determination of nicotine in spray and in e-cigarette cartridges The aim of this diploma thesis was the development and validation of HPLC method for the determination of nicotine in mouth spray and in e-cigarette cartridges. The development was based on available methods that deal with HPLC analysis of nicotine and its related compound cotinine. The optimum conditions were found using XTerra® MS C18 column (100 x 4.6 mm, 3.5 µm) with mobile phase acetonitrile : 10mM ammonium acetate pH 9 (20 : 80, v/v). The flow-rate was 1 ml/min and the wavelenght of the detector was set up at 260 nm. Trimethoprim was used as an internal standard. Under these conditions nicotine, cotinine and trimethoprim were separated within 5 minutes. It was impossible to determine cotinine in the analysis of mouth spray and chosen electronic cigarette cartridges due to coelution of used excipients. Therefore the developed HPLC method was validated and system suitability parameters were evaluated only for nicotine. The results of validation demonstrated, that the method provides...
Development and validation of method for determination of furosemide and its degradation product in pharmaceutical formulations
Břežná, Tereza ; Matysová, Ludmila (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Břežná Advisor: PharmDr. Ludmila Matysová, Ph.D. Thesis name: Development and validation of method for determination of furosemide and its degradation product in pharmaceutical formulations This thesis is focused on development and validation of a method for determination of furosemide and its degradation product in pharmaceutical formulations. The development was based on the HPLC method, which uses the mixture of 0.1 % formic acid and methanol in the 30:70 ratio as the mobile phase. The method development has been namely based on the mobile phase composition and analysis temperature conditions optimizing. In the final version was used the HPLC method with UV-VIS detection at 270 nm. The analysis was performed at the laboratory temperature using isocratic elution and C18 15 x 4.6 mm column with 5 μm particle size. The mixture of pH 5.75 formic acid and acetonitrile in the 65:35 ratio and at 1.5 ml/min flow rate has been selected as the mobile phase. The validation has proven that this method provides precise and acurate results and therefore it is suitable for furosemide determination in the solutions of medicinal products.

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