National Repository of Grey Literature 7 records found  Search took 0.00 seconds. 
Dynamika pohybu a umlčování transgenních lokusů HP1 v živých buňkách
Ondřej, Vladan ; Kozubek, Stanislav ; Lukášová, Emilie ; Matula, Pa. ; Matula, Pe. ; Kozubek, Michal
Intranuclear localization and mobility of Cy3 labelled transgene loci were studied in living cells. Observations showed occurrence of several transgene copies in each cell nucleus. The majority of copies were localized in the centromeric heterochromatin defined by HPlbeta-GFP fusion protein, the minority of copies in euchromatin. The tracking of loci showed restricted diffusive motion of these in the short-time range. During long-time observation of HPlbeta domains and transgene loci, we have found that in most cases the proximity of these objects decreased within time. The changes of positions of HPl domains were very small but transgene loci displayed directional movement towards the relevant HPl domain. Our data records support the idea that the cell nucleus consists of several separated higher-order compartments. The genes relocate within these compartments, which is finally connected with changes of their expression status.
Obrazová analýza pohybu struktur uvnitř buněk
Matula, Pe. ; Ondřej, Vladan ; Kozubek, Stanislav
Analysis of time-lapse series of images of living cells requires automation. This contribution concerns the main features of the software package developed for this purpose in our laboratory. Some improvements of our recently published method for tracking of sub-cellular structures based on graph theory are presented. The behaviour of the method is evaluated on images of centromeric heterochromatin defined by HPlbeta-GFP fusion protein. The results show that the method is very well applicable for this type of data.
Využití vysokorozlišovací cytometrie ke studiu živých buněk
Vařecha, M. ; Amrichová, J. ; Ondřej, Vladan ; Lukášová, Emilie ; Kozubek, Stanislav ; Matula, Pa. ; Matula, Pe. ; Kozubek, Michal
The fluorescent proteins are important innovation in the field of cell biology and in vivo experiments that use fluorescent proteins bring new interesting results from a different point of view than in vitro and in situ experiments. This presentation will introduce our automated 2D/3D high-resolution time-lapse image acquisition and analysis of living cells transfected with plasmids encoding fusion proteins or dyed with fluorescence probes. One of our in vivo projects, we will mention, is focused on the study of mitochondrial and nuclear apoptotic processes in living cells. Another in vivo project concentrates on the study of topography of telomeres and incidence of telomere-association phenomenon in various types of human tumor and healthy cells. In our experiments, cells are stably or transiently transfected with plasmid DNAs coding mitochondrial apoptogenic or telomere-binding proteins with fluorescent proteins.

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1 Matula, Pa.
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