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Significance of neural antibodies in patients with drug refractory epilepsy
Mojžišová, Hana ; Marusič, Petr (advisor) ; Nytrová, Petra (referee) ; Štourač, Pavel (referee)
Significance of neural antibodies in patients with drug refractory epilepsy Abstract One possible etiology of epilepsy is autoimmune, which was included in the classification of epilepsies in its latest revision published in 2017 by the International League Against Epilepsy. The aim of this study was to evaluate the prevalence of neural autoantibodies in patients with epilepsy and to expand the diagnostic possibilities in the Czech Republic. Together with the Dutch authors, we described the prevalence of neural autoantibodies in Dutch and Czech patients with focal epilepsy of unknown etiology and proposed a scoring system to identify patients prioritized for testing for these antibodies. In a Czech cohort of patients, we assessed the presence of antibodies in serum and CSF of patients with drug refractory epilepsy. The results of both these studies showed a lower prevalence of neural autoantibodies than previously described in patients with epilepsy, due to a more rigorous methodology with verification of positive results by a second method. Since neural autoantibodies are primarily associated with autoimmune encephalitis, we also investigated this group, including an assessment of the presence of epileptic seizures in the clinical profile. Together with the Swedish authors, we investigated the proteomics...
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Prognostic markers of disease course in early stages of multiple sclerosis
Petržalka, Marko ; Marusič, Petr (advisor) ; Taláb, Radomír (referee) ; Lízrová, Jana (referee)
Prognostic markers of disease course in early stages of multiple sclerosis ABSTRACT This thesis analyzes the biomarkers of unfavorable prognosis assessed at early stages of multiple sclerosis. Because specific biomarkers often providerather complex information,the theoretical part provides a summary of biomarkers used not only in prognosis assessment, but also in diagnostics and therapy monitoring. In the empirical part, we have investigatedthe prognostic value of intrathecal synthesis of class M immunoglobulins, serum and cerebrospinal fluid concentrations of interleukin 2, interleukin 6, interleukin 10, chitinase 3-like 2 protein and neurofilament heavy chains, assessed from samples acquired during diagnostic procedures. 58 patients early after the first manifestation of multiple sclerosis were included. All patients initiated therapy with glatiramer acetate, teriflunomide, or interferon beta. To assess the evolution of the disease, we followed the patients clinically and with MRI for two years. Interleukin 2 to interleukin 6 ratio of cerebrospinal fluidvalues ˂0.48 (p=0.0028), interleukin 2 in cerebrospinal fluid ≥1.23 (p=0.0260), chitinase 3-like 2 in cerebrospinal fluid ≥7900 (p=0.0330), age at clinical onset ˂22 years (p=0.0312) and baseline Expanded Disability Status Scale ≥1.5 (p=0.0481), proved to...
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Functional cognitive mapping of temporopolar regions in temporal lobe epilepsy patiens
Amlerová, Jana ; Marusič, Petr (advisor) ; Mareš, Jan (referee) ; Klement, Daniel (referee)
The aim of this thesis is to summarize basic knowledge and bring some new data about the temporal pole function in temporal lobe epilepsy patients focusing on the less explored domains -famous face identification, emotion recognition, social cognition and spatial navigation. These functions are not routinely tested in standard neuropsychological assessment however the impairment of these functions may lead to severe dysfunctions in everyday life of the temporal lobe epilepsy patients. Powered by TCPDF (www.tcpdf.org)
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REM sleep behavior disorder:Characteristics of polysomnographic and behavioral manifestations.
Nepožitek, Jiří ; Šonka, Karel (advisor) ; Bušková, Jitka (referee) ; Marusič, Petr (referee)
REM sleep behavior disorder: Characteristics of polysomnographic and behavioral manifestations Abstract REM sleep behavior disorder (RBD) is a disease characterized by abnormal motor activity corresponding to the dream content. REM sleep without atonia (RWA) and behavioral manifestations are the main features registered by video-polysomnography (PSG). Because idiopathic RBD (iRBD) is considered as prodromal stage of synucleinopathies, the direction of current research is the search for markers of early conversion. The goal of this study was to observe the group of patients with iRBD with regard to the development of manifest neurodegenerative disease, to find and test a new polysomnographic marker of phenoconversion, to perform analysis of the movements registered by video and to quantify excessive fragmentary myoclonus (EFM), which is a frequent finding in neurodegenerative processes. A total of 55 patients with iRBD were observed for 2.3±0.7 years. The annual conversion rate was 5.5%. Mixed RWA, representing simultaneous occurrence of phasic and tonic RWA, was suggested as a new marker of phenoconversion. Converted patients showed a higher mixed RWA (p=0.009) and the ROC analysis confirmed that mixed RWA is the best predictive marker of conversion among other RWA types (AUC 0.778). An average of...
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Epileptogenesis in autoimmune encephalitis associated with autoantibodies
Elišák, Martin ; Marusič, Petr (advisor) ; Nytrová, Petra (referee) ; Pail, Martin (referee)
9 Abstract The aim of this thesis is the role of neuronal antibodies in epileptogenesis. In the first phase of research we focused on 1) characteristics of epileptic seizures in patients with autoimmune encephalitis, 2) implementation of tissue brain assay as novel antibodies detection method in the Czech Republic, 3) profile of chemo- and cytokines in different autoimmune encephalitis stages, 4) genetic risk factors of autoimmune encephalitis development. After mastering the detection methods, we found neural antibodies in 5 % of temporal lobe epilepsy patients. Immunotherapy response in some cases and lack of antibodies positivity in subgroup of temporal lobe epilepsy patients of known aetiology suggests their pathogenic role. Because seropositive and seronegative patients are difficult to distinguish by clinical characteristics in temporal lobe epilepsy patients (statistical significant differences were only in two of 14 characteristics), neural antibodies could be potential biomarker of autoimmune epilepsy. However, the investigation as a screening tool in all temporal epilepsy patient are too high, we focused on determination of clinical risk factors leading to identify the indication of antineural antibodies investigation. In the cohort of patients with focal epilepsy of unknown aetiology antineural...
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REM sleep behavior disorder:Characteristics of polysomnographic and behavioral manifestations.
Nepožitek, Jiří ; Šonka, Karel (advisor) ; Bušková, Jitka (referee) ; Marusič, Petr (referee)
REM sleep behavior disorder: Characteristics of polysomnographic and behavioral manifestations Abstract REM sleep behavior disorder (RBD) is a disease characterized by abnormal motor activity corresponding to the dream content. REM sleep without atonia (RWA) and behavioral manifestations are the main features registered by video-polysomnography (PSG). Because idiopathic RBD (iRBD) is considered as prodromal stage of synucleinopathies, the direction of current research is the search for markers of early conversion. The goal of this study was to observe the group of patients with iRBD with regard to the development of manifest neurodegenerative disease, to find and test a new polysomnographic marker of phenoconversion, to perform analysis of the movements registered by video and to quantify excessive fragmentary myoclonus (EFM), which is a frequent finding in neurodegenerative processes. A total of 55 patients with iRBD were observed for 2.3±0.7 years. The annual conversion rate was 5.5%. Mixed RWA, representing simultaneous occurrence of phasic and tonic RWA, was suggested as a new marker of phenoconversion. Converted patients showed a higher mixed RWA (p=0.009) and the ROC analysis confirmed that mixed RWA is the best predictive marker of conversion among other RWA types (AUC 0.778). An average of...
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Functional cognitive mapping of temporopolar regions in temporal lobe epilepsy patiens
Amlerová, Jana ; Marusič, Petr (advisor) ; Mareš, Jan (referee) ; Klement, Daniel (referee)
The aim of this thesis is to summarize basic knowledge and bring some new data about the temporal pole function in temporal lobe epilepsy patients focusing on the less explored domains -famous face identification, emotion recognition, social cognition and spatial navigation. These functions are not routinely tested in standard neuropsychological assessment however the impairment of these functions may lead to severe dysfunctions in everyday life of the temporal lobe epilepsy patients. Powered by TCPDF (www.tcpdf.org)
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