National Repository of Grey Literature 78 records found  beginprevious31 - 40nextend  jump to record: Search took 0.01 seconds. 
Structure and function relationships of model hemoproteins
Lengálová, Alžběta ; Martínková, Markéta (advisor) ; Hudeček, Jiří (referee) ; Muchová, Lucie (referee)
Heme is one of the most important and most studied cofactors that are essential for proper function of many proteins. Heme-containing proteins comprise of a large group of biologically important molecules that are involved in many physiological processes. The presented dissertation is focused on two groups of heme sensor proteins, namely prokaryotic heme-based gas sensors and eukaryotic heme-responsive sensors. Heme-based gas sensors play an important role in regulation of many bacterial processes and consist usually of two domains, a sensor domain and a functional domain. The dissertation thesis aims at the study of two model bacterial heme-based gas sensors, histidine kinase AfGcHK and diguanylate cyclase YddV, in order to elucidate their mechanism of interdomain signal transduction. Using X-ray crystallography and hydrogen-deuterium exchange coupled to mass spectrometry approaches, significant differences in the structure of the AfGcHK protein between the active and inactive forms were described. The signal detection by the AfGcHK sensor domain affects the structural properties of the protein, and these conformational changes then have indirect impact on the enzyme activity of the functional domain. Further, the dissertation pays more attention to the effect of a sensor domain dimerization...
Biochemical characterization of a model heme sensor protein
Tajovská, Eva ; Martínková, Markéta (advisor) ; Jeřábek, Petr (referee)
Hemoproteins play a lot of important roles within the living organism. One of these being the sensor function - heme sensor proteins are able to detect the changing concentration of heme in organisms. Heme itself serves as a signalling molecule for these proteins. Heme-based gas sensor proteins use a biatomic gas molecule as a signalling molecule. Signal transduction begins once the gas molecule is bound to the heme molecule which is already tightly bound in the protein sensing domain. Both these types of signalling regulate a number of physiological processes in the prokaryotic and eukaryotic organisms. The theoretical part of the bachelor thesis summarizes recent scientific studies on heme sensor proteins, while the experimental part focuses on the properties of a specific model sensor hemoprotein. A direct oxygen sensor from E. coli (EcDOS) was selected as a model protein. Furthermore, the thesis deals with a truncated form of the EcDOS protein, an isolated sensor domain with a PAS structure (EcDOS-PAS). The experimental part aimed at expressing and isolating of both mentioned proteins from E. coli BL-21 (DE3) cells. Finally, both the EcDOS and EcDOS-PAS proteins were preliminarily characterised and their properties compared. The yield of the EcDOS-PAS was several times higher than the yield of...
Chemical and biochemical transformation of bioactive compounds
Šimášková, Ema ; Sokolová, Romana (advisor) ; Martínková, Markéta (referee)
Xenobiotics, such as pharmaceuticals, food additives, environmental pollutants, and dietary bioactive compounds in organism are metabolized by various enzymes, resulting in their bioactivation or detoxification. Identification of structure of resulting metabolites is important for their detection in bodily fluids and tissues for diagnostic and forensic purposes. This thesis reviews known biochemical processes and enzymes involved in xenobiotic metabolism, including cytochromes 450 (CYP) and flavine monooxygenases (FMO). Given that biochemical reactions are to a major extent composed of electron-transfer reactions (i.e. oxidation and reduction), the thesis includes a section dealing with the practical approaches to determination of the oxidative or reductive mechanism of bioactive compounds.
Case Study of Physiotherapy Treatment of a Patient after Th/L Junction Stabilization
Martinková, Markéta ; Maršáková, Kateřina (advisor) ; Hamouzová, Dita (referee)
Autor: Markéta Martinková Title: Case Study of Physiotherapy Treatment of a Patient after Th/L Junction Stabilization Objectives: The main aim of this thesis is to gain theoretical knowledge about anatomy, biomechanics, spine construction and injury mechanisms. This bachelor thesis is dedicated to a solution to spine injuries, whether it's surgical or conservative. Common physiotherapeutic procedures used in the treatment of spinal injuries will be mentioned. Special part of this thesis is dedicated to physiotherapy care of a patient after spinal injury and after following stabilization of Th/L junction stabilization. I was working on this special part during my professional experience in the field. Key words: spine, stabilization, physiotherapy, case report
Antioxidant and antiinflammatory effects of bilirubin.
Valášková, Petra ; Muchová, Lucie (advisor) ; Martínková, Markéta (referee) ; Dvořák, Karel (referee)
For a long time, bilirubin (BR) has been considered a waste molecule with potential toxic effects especially on the central nervous system. Later, it was found that BR exhibited cytoprotective effects and mildly elevated BR levels showed antioxidant, anti-inflammatory and immunomodulatory properties, however, exact mechanisms of the anti-inflammatory actions of BR have not been fully understood yet. The main aim of this study was to assess the protective effects of BR using experimental in vivo and in vitro models in relation to inflammation and oxidative stress. Partial goal was to establish validated analytical method for determination of BR and lumirubin. Gunn and heterozygous rats were treated with lipopolysaccharide (LPS, 6 mg/kg, IP) or vehicle (saline). After 12 hours, blood and organs were collected for analyses of inflammatory and hepatic injury markers. Primary rat hepatocytes were treated with BR and TNF-α, HepG2 and SH-SY5Y cell lines were treated with BR and chenodeoxycholic acid. LPS-treated Gunn rats had a significantly decreased inflammatory response and hepatic injury compared to LPS- treated normobilirubinemic controls. We found different profile of leukocytes subsets and decreased systemic mRNA expressions and concentrations of IL-6, TNF-α, IL-1β and IL-10 in Gunn rats. Hepatic mRNA...

National Repository of Grey Literature : 78 records found   beginprevious31 - 40nextend  jump to record:
See also: similar author names
3 MARTÍNKOVÁ, Markéta
7 MARTÍNKOVÁ, Michaela
1 Martinková, Magda
3 Martinková, Markéta
4 Martinková, Martina
7 Martinková, Michaela
1 Martinková, Milada
1 Martinková, Milena
8 Martinková, Monika
2 Martínková, Magdalena
2 Martínková, Marie
19 Martínková, Marta
7 Martínková, Michaela
8 Martínková, Monika
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