National Repository of Grey Literature 26 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Biologically active metabolites of plants. 5. Alkaloids from Zanthoxylum nitidum and their biological activity.
Marková, Lenka ; Cahlíková, Lucie (advisor) ; Jahodář, Luděk (referee)
Marková L.: Biologically active metabolite sof plants 4. Alkaloids from Zanthoxylum nitidum and their biological activity. Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2012. Diploma thesis, 67 s. The aim of the diploma thesis was to process the dried drug of Zanthoxylum nitidum and to obtain the total extract. By means of preparative thin layer chromatogramy one alkaloid was isolated and based on structural analysis (NMR, MS) and comparison of data from the literature identified as (-)-edulinine. This alkaloid was tested for its inhibitory activity against human erytrocyte acetylcholinesterase activity (HuAChE) and human plasma butyrylcholiesterase (HuBuChE). The measured values for (-)-edulinine were IC50 HuAChE > 1000 µM a IC50 HuBuChE > 1000 µM. Galanthamin (IC50, HuAChE = 1,710 ± 0,065µM, IC50 HuBuChE = 42,30 ± 1,30 µM) and huperzin A (IC50, HuAChE = 0,033 ± 0,001µM, IC50 HuBuChE = >1000 µM) were used as positive standarts which are used in therapy of Alzheimer's disease. In comparison of standarts (-)-edulinine was inactive and it can't be considered as perspective substance in treatment of Alzheimer's disease. The alkaloid was also subjected to study of its antioxidant activity. The resulting EC50 value...
Analysis of the evaluation criteria of kindergarten headmasters by founders in Pardubický region
Marková, Lenka ; Trunda, Jiří (advisor) ; Trojanová, Irena (referee)
TITLE: Analysis of the evaluation criteria of kindergarten headmasters by founders in Pardubicky region AUTHOR: Bc. Lenka Marková DEPARTMENT: School management centre SUPERVISOR: Mgr. Jiří Trunda ABSTRACT: This thesis analyzes the current criteria for evaluating the work of kindergarten headmasters. The criteria were created by community representatives as founders of the kindergarten. The thesis also focuses on identifying indicators leading to the fulfillment of the evaluation criteria. Monitoring headmasters views on the current criteria, which they are valued, is the part of research. Through the analysis criteria, surveys and informal interviews with representatives of the founders and headmasters the deficiencies in the structure and list of criteria were found. The research has shown inconsistency and formality outlined evaluation criteria with absence of their indicators. Based on the findings was recommended evaluation criteria to include all areas of the kindergarten headmasters. Indicators, that show the way to meet the criteria, were also suggested. This thesis aims to draw attention to current issues in evaluating the work of headmastrers at the regional education as well as to need for systemic solutions in this area. This thesis can be used as the theme for the creation of evaluation criteria...
The role of mitochondria in the pathogenesis
Marková, Lenka ; Doležal, Pavel (advisor) ; Verner, Zdeněk (referee)
This thesis is focused on the existing data referring to role of mitochondria in the pathogenesis of several important diseases. It introduces mitochondrial proteins, their role and metabolism, dysfunction of which is behind pathogenetic processes. The thesis also summarizes possible mitochondrial damage, its progress and consequences, which can lead to diseases or aggravate their process. It also pays attention to the role of mitochondria during oncogenesis as well as important neurodegenerative disorders such as Alzheimer's or Parkinson's disease, Charcot-Marie-Tooth disease, amyotrophic lateral sclerosis or autosomal dominant optic atrophy. The thesis mentions the role of disrupted mitochondrial dynamics in type 2 Diabetes. In conclusion the thesis mentions the role of mitochondria and their damage in relation to infection by the parasites Toxoplasma gondii and Trypanosoma cruzi.
Naegleria: from free-living protist to brain eating deadly pathogen
Matějková, Magdalena ; Mach, Jan (advisor) ; Marková, Lenka (referee)
N. fowleri is mostly a free-living amoeboflagellate which, in favorable conditions, can occasionally adopt a parasitic lifestyle. In such cases, the amoeba becomes causative agent of primary amoebic meningoencephalitis, a severe and largely fatal disease occurring in several mammals including humans. The treatment of this disease is complicated by many unwanted side-effects of available drugs and a lack of prompt diagnostic methods. Within the Naegleria genus, N. fowleri is the only human pathogen. According to up-to-date knowledge, the pathogenicity of this species is due to its metabolic properties as well as to a particular set of proteins expressed by this protist. Besides a wide scale of enzymes, some of these proteins participate in the formation of cellular structures which are crucial for the pathogenesis. All these particularities should be targeted in a search for a better treatment. The thesis is focusing on a comparison of N. fowleri with related species N. gruberi and N. lovaniensis, stressing out the differences of N. fowleri from these non-pathogenic species. Key words: Naegleria fowleri, primary amoebic meningoencephalitis, immune response, pathogenesis, facultative parasitism
Five-hole flute introduction programme for pre-school children
MARKOVÁ, Lenka
Bachelor thesis is focused on the benefits of playing the five-hole recordernot only in the musical development area, but also in the psychological and somatic one. The content of the theoretical part is an introduction to musical activities in kindergarten, possibilities and variations of the perception of music, and its importance in preschool education. It is also mentioned, how vital it is to give psychological support to the child while she or he copes with the various pitfalls of learning to play the five-hole recorder. Also, it is important to not only fulfill the general and partial educational objectives based on the Framework Educational Program for Preschool Education but also to find a suitable way to meet these objectives in all educational areas. Since the children's five-hole recorder is still unknown to a lot of people, this thesis also describes it. It puts it into a group of better-known musical wind instruments and explains the benefits to preschool children. Knowing this, it is beneficial to understand the historical facts about the formation and classification of some musical instruments. The practical part describes the specific work with a group of preschool children attending the five-hole recorder lessons. Described is also an approach toward individual children emphasizing their unique peculiarities. The findings thus show what goals can be achieved after six months of work with these children, and the most significant progress that can be observed in them. The thesis is accompanied by a book of songs and some of the tools I use in my lessons.
Proteome of the encystation-specific vesicles in Giardia intestinalis.
Marková, Lenka ; Doležal, Pavel (advisor) ; Tůmová, Pavla (referee)
The process of encystation is a vital and important part of life cycles of many organisms. One of them is Giardia intestinalis, a significant single cell parasite of vertebrates, including man. During the process of encystation there are unusual organelles, called encystation vesicles. These serve to accumulate material for the future cyst wall. The main proteins found in encystation vesicles that compose the cyst wall, are CWP1, CWP2 and CWP3. This work is focused on these proteins. The structure and properties of these proteins were used to study the main aim of this work, that is the identification of other proteins associated with the encystation vesicles. In the experimental part, a system was created, that allows targeted characterization of proteins and cell compartments, by utilization of biotin ligase of Escherichia coli. For this systém, we created specific constructs, that were successfully inserted into plasmids and into G. intestinalis cells. For the intention of cloning, a method of direct site mutagenesis was optimalized. The expression of chosen proteins was detected successfully during the in vitro encystation. Aside, it was proven that CWP1 forms complexes with an unknown protein. This work relates the composition of cyst wall of G. intestinalis with other components identified in...
Firm's Competitive Strategy
Marková, Lenka ; Jelínková, Martina (referee) ; Breinek, Pavel (advisor)
The diploma thesis is focused on creation of competitive strategy of medium size firm. It analyzes current status of a company, which is specialized in the manufacture and sale of electric motors. The thesis investigates impal of external eviroment on the firm and it also analyses internal enviroment. The thesis defines strategic targets, contains proposal of competitive strategy of the company and time of its implementation.
The role of mitochondria in the pathogenesis
Marková, Lenka ; Doležal, Pavel (advisor) ; Verner, Zdeněk (referee)
This thesis is focused on the existing data referring to role of mitochondria in the pathogenesis of several important diseases. It introduces mitochondrial proteins, their role and metabolism, dysfunction of which is behind pathogenetic processes. The thesis also summarizes possible mitochondrial damage, its progress and consequences, which can lead to diseases or aggravate their process. It also pays attention to the role of mitochondria during oncogenesis as well as important neurodegenerative disorders such as Alzheimer's or Parkinson's disease, Charcot-Marie-Tooth disease, amyotrophic lateral sclerosis or autosomal dominant optic atrophy. The thesis mentions the role of disrupted mitochondrial dynamics in type 2 Diabetes. In conclusion the thesis mentions the role of mitochondria and their damage in relation to infection by the parasites Toxoplasma gondii and Trypanosoma cruzi.
Biologically active metabolites of plants. 5. Alkaloids from Zanthoxylum nitidum and their biological activity.
Marková, Lenka ; Cahlíková, Lucie (advisor) ; Jahodář, Luděk (referee)
Marková L.: Biologically active metabolite sof plants 4. Alkaloids from Zanthoxylum nitidum and their biological activity. Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové, 2012. Diploma thesis, 67 s. The aim of the diploma thesis was to process the dried drug of Zanthoxylum nitidum and to obtain the total extract. By means of preparative thin layer chromatogramy one alkaloid was isolated and based on structural analysis (NMR, MS) and comparison of data from the literature identified as (-)-edulinine. This alkaloid was tested for its inhibitory activity against human erytrocyte acetylcholinesterase activity (HuAChE) and human plasma butyrylcholiesterase (HuBuChE). The measured values for (-)-edulinine were IC50 HuAChE > 1000 µM a IC50 HuBuChE > 1000 µM. Galanthamin (IC50, HuAChE = 1,710 ± 0,065µM, IC50 HuBuChE = 42,30 ± 1,30 µM) and huperzin A (IC50, HuAChE = 0,033 ± 0,001µM, IC50 HuBuChE = >1000 µM) were used as positive standarts which are used in therapy of Alzheimer's disease. In comparison of standarts (-)-edulinine was inactive and it can't be considered as perspective substance in treatment of Alzheimer's disease. The alkaloid was also subjected to study of its antioxidant activity. The resulting EC50 value...

National Repository of Grey Literature : 26 records found   1 - 10nextend  jump to record:
See also: similar author names
1 MARKOVÁ, Ladislava
29 MARKOVÁ, Lenka
1 MARKOVÁ, Libuše
46 MARKOVÁ, Lucie
1 Marková, Lada
3 Marková, Leona
1 Marková, Ljuba
46 Marková, Lucie
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