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Preeclamsia and selected immunogenetic factors
Hradecký, Libor ; Ulčová-Gallová, Zdeňka (advisor) ; Madar, Jindřich (referee) ; Oborná, Ivana (referee)
Preeclampsia and selected immunogenetic factors Our objective was to evaluate plasma levels of the eight most common antiphospholipid antibodies (antiphosphatidylserine, antiphosphatidylethanolamine, antiphosphatidylinositol, antiphosphatidylglycerol, antiphosphatidic acid, antiannexin V, anticardiolipin and anti 2-glycoprotein I antibodies) by ELISA method and selected inherited thrombophilia (F V- Leiden mutation, FII mutation G20210A, C677T and A1298C variants of the gene for methylene tetrahydrofolate reductase-MTHFR) by DNA analysis of peripheral blood lymphocytes using the real-time PCR in fifty-five women with preeclampsia in the period immediately before urgent termination of pregnancy. Fifty-five healthy women without preeclampsia was considered as a controll group. Entered data were examined using a non-parametric Wilcoxon's test, univariate analysis were perfomed using the Fisher's exact test and statistical dependence between variables was assessed using Spearman's rank correlation coefficient. We demonstrated that women with preeclampsia had significantly higher levels of anticardiolipin antibodies in the isotope IgG (p <0.01) and IgM (p <0.01), elevated levels of antiphosphatidylserine antibodies in the isotope IgG(p <0.01) and antiethanolamine antibodies in the isotope IgM (p <0.01) when...
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The role of immune system factors in IVF/ET success
Růžičková, Žaneta ; Madar, Jindřich (advisor) ; Pěknicová, Jana (referee)
5 Abstract The aim of this study was to evaluate the presence of receptors (CD16, CD161, NKG2D, KIR2DL4 and CD85j) on the surface of immunocompetent cells and whether their presence is involved in the in vitro fertilization (IVF) and following embryotransfer (ET) success. We observed significant difference in the age of successful versus unsuccessful patients, which is confirmed by former studies on age-dependence of IVF success. The result from cytotoxic assay confirmed, that neither the stimulation protocol, nor the PBMC source have any effect on effector function. Afterwards, we performed a large-scale analysis of surface markers, starting with healthy donors, compared to patients. Both groups of patients have lower counts of HLA-G binding KIR2DL4 or CD85j receptors bearing cells. Next we observed the expression of these two receptors on various subpopulations of cells (CD56+dim , CD56+bright , T helpers and cytotoxic T cells) and their counts. Here we observed differences in CD56 surface expression on CD56+ bright NK cells, the levels of CD56+dim NK cells in PBMC (CD16 expression on this population), levels of CD56+dim Next step was to compare the levels of cell populations between the successful and unsuccessful patients. We observed an increased surface expression of KIR2DL4 on lymphocytary PBMC of...
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History of Slovak pietism and its influence on society and culture
Maďar, Jaroslav ; Lášek, Jan Blahoslav (advisor) ; Weis, Martin (referee) ; Hanes, Pavel (referee)
Jaroslav Maďar Dejiny slovenského pietizmu a jeho vplyv na spoločnosť a kultúru History of Slovak pietism and its influence on society and culture The dissertation entitled The History of Slovak Pietism and Its Influence on Society and Culture is contribution to the history of church in Slovakia. The work is divided into five chapters. The first two ones deal with the survey, assessment of the past and contemporary research in this field of study and the relationship between worldwide and Slovak Pietism. The core of the work are the next three chapters devoted to the inception (1675-1707), growth (1708-1757) and abating (1758-1803) of Slovak Pietism with accent of personalities and their works of each era.
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Observation of human sperm cell immunogenic properties
Cibulka, Jan ; Ulčová-Gallová, Zdeňka (advisor) ; Fingerová, Helena (referee) ; Madar, Jindřich (referee) ; Šíma, Petr (referee)
The issue of human infertility is becoming increasingly pressing, especially in so- called industrially developed countries. Formally, we can share the infertility attributes between the male and the female partner (as a male and female infertility factor), but the problem finally affects the whole parental couple. Perhaps, due to the complexity of the female reproductive tract, it is estimated that over 40% of the reproductive failure is hidden in one or more of its parts. The "strong point" of the male factor is derived from the pathological sperm count. For the remaining 15 to 20% of causes, whether morphological or functional, cannot be to find either clearly or not at all. We talk about the otherwise unexplained infertility, that can be caused in up to 30% immunologically. Due to broad concept of immunology of reproduction, we have chosen literally as a probe into this issue in all respects unique spermatic cell that represents not only a carrier of genetic information, but also a set of antigenic structures. This cell seems to be perfect for this task, because of its availability to most basic laboratory techniques. We will try to familiarize ourselves with some of the obstacles that may prematurely end its mission, especially with the antibodies against its antigenic structures (ASA -...
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The woman at immunological risk and her infant
Mocková, Alice ; Ulčová-Gallová, Zdeňka (advisor) ; Madar, Jindřich (referee) ; Šedivá, Anna (referee)
Women in childbearing age are often affected by autoimmune diseases (AD) associated with the presence of antiphospholipid antibodies (aPL) that may influence further develop-ment of their children. The primary objective of our prospective study was to determine the presence of the following aPL: anti β2 glycoprotein I (anti-β2GPI), anticardiolipin (aCL), antiphosphatidylserine, antiphosphatidylinositol, antihospha- tidylethanolamine, antiphosphatidylglycerol, antiphosphatidic acid, antiannexin V in mothers with defined AD and their children after birth, at 6 and 12 months of life, and to compare the incidence of aPL with a control group. A secondary objective of the study was a 2-year follow-up of children born to aPL negative and aPL positive mothers with AD in order to detect the possible impact of maternal AD on the health of the offspring. In children, we analysed anthropometric data, blood cell count, cerebral and abdominal ultrasound examination, transient evoked otoacoustic emission test (TEOAE), electrocardiograph (ECG), the presence and kinetics of aPL. At the age of 2 years the Bayley Scales of Infant Development (BSID-II) were used for children's assessment of motor, language and cognitive development. 31 mothers from the total examined 82 aPL positive women with AD delivered 34 neonates...
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The woman at immunological risk and her infant
Mocková, Alice ; Ulčová-Gallová, Zdeňka (advisor) ; Madar, Jindřich (referee) ; Šedivá, Anna (referee)
Women in childbearing age are often affected by autoimmune diseases (AD) associated with the presence of antiphospholipid antibodies (aPL) that may influence further develop-ment of their children. The primary objective of our prospective study was to determine the presence of the following aPL: anti β2 glycoprotein I (anti-β2GPI), anticardiolipin (aCL), antiphosphatidylserine, antiphosphatidylinositol, antihospha- tidylethanolamine, antiphosphatidylglycerol, antiphosphatidic acid, antiannexin V in mothers with defined AD and their children after birth, at 6 and 12 months of life, and to compare the incidence of aPL with a control group. A secondary objective of the study was a 2-year follow-up of children born to aPL negative and aPL positive mothers with AD in order to detect the possible impact of maternal AD on the health of the offspring. In children, we analysed anthropometric data, blood cell count, cerebral and abdominal ultrasound examination, transient evoked otoacoustic emission test (TEOAE), electrocardiograph (ECG), the presence and kinetics of aPL. At the age of 2 years the Bayley Scales of Infant Development (BSID-II) were used for children's assessment of motor, language and cognitive development. 31 mothers from the total examined 82 aPL positive women with AD delivered 34 neonates...
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Observation of human sperm cell immunogenic properties
Cibulka, Jan ; Ulčová-Gallová, Zdeňka (advisor) ; Fingerová, Helena (referee) ; Madar, Jindřich (referee) ; Šíma, Petr (referee)
The issue of human infertility is becoming increasingly pressing, especially in so- called industrially developed countries. Formally, we can share the infertility attributes between the male and the female partner (as a male and female infertility factor), but the problem finally affects the whole parental couple. Perhaps, due to the complexity of the female reproductive tract, it is estimated that over 40% of the reproductive failure is hidden in one or more of its parts. The "strong point" of the male factor is derived from the pathological sperm count. For the remaining 15 to 20% of causes, whether morphological or functional, cannot be to find either clearly or not at all. We talk about the otherwise unexplained infertility, that can be caused in up to 30% immunologically. Due to broad concept of immunology of reproduction, we have chosen literally as a probe into this issue in all respects unique spermatic cell that represents not only a carrier of genetic information, but also a set of antigenic structures. This cell seems to be perfect for this task, because of its availability to most basic laboratory techniques. We will try to familiarize ourselves with some of the obstacles that may prematurely end its mission, especially with the antibodies against its antigenic structures (ASA -...
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History of Slovak pietism and its influence on society and culture
Maďar, Jaroslav ; Lášek, Jan Blahoslav (advisor) ; Weis, Martin (referee) ; Hanes, Pavel (referee)
Jaroslav Maďar Dejiny slovenského pietizmu a jeho vplyv na spoločnosť a kultúru History of Slovak pietism and its influence on society and culture The dissertation entitled The History of Slovak Pietism and Its Influence on Society and Culture is contribution to the history of church in Slovakia. The work is divided into five chapters. The first two ones deal with the survey, assessment of the past and contemporary research in this field of study and the relationship between worldwide and Slovak Pietism. The core of the work are the next three chapters devoted to the inception (1675-1707), growth (1708-1757) and abating (1758-1803) of Slovak Pietism with accent of personalities and their works of each era.
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Preeclamsia and selected immunogenetic factors
Hradecký, Libor ; Ulčová-Gallová, Zdeňka (advisor) ; Madar, Jindřich (referee) ; Oborná, Ivana (referee)
Preeclampsia and selected immunogenetic factors Our objective was to evaluate plasma levels of the eight most common antiphospholipid antibodies (antiphosphatidylserine, antiphosphatidylethanolamine, antiphosphatidylinositol, antiphosphatidylglycerol, antiphosphatidic acid, antiannexin V, anticardiolipin and anti 2-glycoprotein I antibodies) by ELISA method and selected inherited thrombophilia (F V- Leiden mutation, FII mutation G20210A, C677T and A1298C variants of the gene for methylene tetrahydrofolate reductase-MTHFR) by DNA analysis of peripheral blood lymphocytes using the real-time PCR in fifty-five women with preeclampsia in the period immediately before urgent termination of pregnancy. Fifty-five healthy women without preeclampsia was considered as a controll group. Entered data were examined using a non-parametric Wilcoxon's test, univariate analysis were perfomed using the Fisher's exact test and statistical dependence between variables was assessed using Spearman's rank correlation coefficient. We demonstrated that women with preeclampsia had significantly higher levels of anticardiolipin antibodies in the isotope IgG (p <0.01) and IgM (p <0.01), elevated levels of antiphosphatidylserine antibodies in the isotope IgG(p <0.01) and antiethanolamine antibodies in the isotope IgM (p <0.01) when...
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