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Effect of cytokinin deficiency on oxidative damage and antioxidant defence during ageing and abiotic stress
Mýtinová, Zuzana ; Wilhelmová, Naďa (advisor) ; Ryšlavá, Helena (referee) ; Prášil, Ilja (referee)
Conchsiom l) Leaves of Úmsgenie tobmo with refuced lwd of CK agedmme sbwly, cmtrary to the contol leaves' AtcKx2 planÉsmaintainedhigher Chl cont€nt ed protein cmtd' md alsohigherphotosyntfuticperformmoein theoldestleaves. 2) We observed elevated mtioxidmt (bott enrynalc md low-moleculr) ftnctional alreadyin theyoungestleavesof AICI(X2. 3) Enhmoementof oertaindioxidmt defencecould be cased oryenscory eff€ct of tb€ decÍeas€ in the level of Úotd CKq assumedas potedial m-ea4nnďic mtioxidmts or as a resultof oxidative súessin&rcedby thelack ďCKs in yorog deve|opingleaves. 4) It is plarrsible thaÍthe higba mtioxidant defeirce migbt comĚute to retÚdation of ageingwitt a consequ€Írc€ of postponedonsetof serrescencein lwes with a defioienoy ofCKs. 5) Tbe responsesof AoE a.tiúties to individual sresses ditreredbetweenleavesmd roots ofboth WT mdAtCKX2 torbacoofuts. ó) The higho toleranceof AICKX2 plms cotúdbe affiib}t€d to noticeably higber activity of GR in leaves md APX SOD md CAT in roots displayed aheady mder cmnol conditions. Althougb rrmsgenic tobacco plmts possess, ď l€ast prtiďy' berer ffiioxidd protection in non-stressedoonditions,they were not oonfirmed to be more tolermt to abiotic sb€ss€s. 7) The ďfference between md WT plants in theř stess Í€spons€s could be associďed with ďvergent seosfiúý to imposed...

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