National Repository of Grey Literature 5 records found  Search took 0.00 seconds. 
Bacterial interactions and secondary metabolites in plant and soil environment
Rapoport, Daria ; Marečková, Markéta (advisor) ; Lovecká, Petra (referee) ; Brabcová, Vendula (referee)
Phylum Actinobacteriota represents one of the major phyla in plant and soil environments. Known for the ability to produce numerous secondary metabolites, Actinobacteriota may affect other bacteria and plants in various ways. The thesis aimed at assessing the connection between the production of secondary metabolites and interactions of actinobacteria in plant and soil environments. In the first part, we assessed how rare actinobacteria from acid soil affect cultivable soil bacteria. We isolated a collection of actinobacteria from acid soil dominated by previously uncultivable lineage and isolated a representative described as a new family Treboniaceae and multiple other potentially novel species of known genera. The genome and metabolome analysis of Trebonia kvetii, sp.nov. gen.nov., demonstrated its ability to produce unusual and potentially novel bioactive metabolites. We designed an experiment where a single strain was co-cultivated with the total soil bacterial community of the same soil in a set up, where a free exchange of diffusible metabolites was allowed between them. Three actinobacteria strains coming from distinct lineages were selected for this interaction. Each strain significantly and specifically affected cultivable bacteria as well as the metabolite pool, part of which was induced...
Utilization of endophytic microorganisms for growth promotion of Miscanthus
Mrnka, Libor ; Frantík, Tomáš ; Schmidt, Christoph Stephan ; Lovecká, P.
Increased survival and aboveground biomass yield of Miscanthus was achieved due to the inoculation of Miscanthus seedlings by a mixed inocula based on the selected endophytic microbes (bacteria and fungi). Inoculated treatments produced 20-90% higher yield of biomass compared to the controls. Importantly, fungal inoculum increased also production of rhizomes and the same trend was observed in the treatment inoculated by the bacterial mixture. Bacterial endophytes also strongly augmented overwintering of the plants on one of the tested model plantations. Because the price of the inocula production is economically feasible and does not substantially increase the plantation establishment costs we hope for fast implementation of the methodology both in CR and beyond.
Real time in vivo monitoring of cytotoxic activity of two different antimicrobial peptides lasioglossin III and lasiocepsin
Tůmová, Tereza ; Lovecká, P. ; Čeřovský, Václav ; Slaninová, Jiřina
Real-time cell analyzer dual-plate (RTCA DP) can be used to dynamically test the cytotoxic activity of potent antimicrobial peptides. In this study, we tested the activity of two different antimicrobial peptides isolated from venom reservoirs of a wild bee against normal mammalian cell line – rat intestinal epithelial cells. The cytotoxic effect monitored by RTCA DP was expressed as IC50 and compared with the results of standard cytotoxic tests.
Následky interakcí mezi rostlinami a mikroby ovlivňujících osud xenobiotik v životním prostředí
Macková, Martina ; Vrchotová, N. ; Beranová, K. ; Najmanová, J. ; Lovecká, P. ; Kochánková, L. ; Sylvestre, M. ; Demnerová, Kateřina ; Macek, Tomáš
The effects and consequences of plant-microbe metabolic interactions influencing fate of xenobiotics in the environment.are discussed, illustrated by our results on PCB degradation by microflora in plant rhizosphere.

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