National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Separation of selected acidic proteins fractions from potato (Solanum tuberosum L.) tubers by chromatographic techniques
LORENC, František
This diploma thesis deals with acidic proteins contained in the potato (Solanum tuberosum) tubers, or rather about their separation with chromatography techniques. For the analysis were chosen tubers of two czech potato varieties, Adéla and Westamyl. From the concentrated potato juice were eliminated basic and most of the patatin proteins with the gravity column chromatography. In the next step were applied the chromatography techniques on the anion and hydroxyapatite columns by the fast protein liquid chromatography (FPLC) system. On the anion column were separated protein fractions which contained proteins of molecular weight in the range of 15 kDa to 60 kDa (Adéla) and 15 kDa to 100 kDa (Westamyl). The most abundant were the proteins with molecular weight 15 kDa a 20 kDa. In the last step was used the mass spectrometry for the identification of chosen protein fractions.
Plant peptides and proteins with antimicrobial activities and possibilities of their application
LORENC, František
This work deals with antimicrobial peptides and proteins in plants. Antimicrobial peptides form one part of non-specific immunity most eukaryotic and prokaryotic organisms. Their primary function is to eliminate the attacks of microbial pathogens, by which are the target organisms exposured. These proteins have low molecular weight. Most of them form a group of PR (Pathogenesis-related) proteins. Thanks to their antimicrobial effects have the antimicrobial peptides and proteins a great potential in pharmaceutical industry, medicine and biotechnology or in protection against plant diseases and also in other applications. The aim of this work is to describe the primary fission of plant antimicrobial peptides and proteins, the mechanisms of their antimicrobial action and characteristics of the main groups of PR-proteins. In the end of this work is described the current research and applications and potential uses in the future.

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2 Lorenc, Filip
4 Lorenc, František
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