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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Analysis of atorvastatine by HPLC with using of fluorescence derivatization
 Líška, Marián ; Šatínský, Dalibor (advisor) ; Solich, Petr (referee)
10.2 Determination of atorvastatin by high performance liquid chromatography with fluorescence detection The main aim of this work was to develop a method for determination of atorvastatin by HPLC, which would be sensitive enough to determine plasmatic concentration of atorvastatin (ng/ml). UV-VIS detection is not sensitive enough for this purpose, therefore fluorescence detection was used. Atorvastatin was derived by 4-brommethyl-6,7-dimethoxycoumarin in N,N- dimethylformamide. Potassium carbonate and 18-crown-6-ether were used as catalysts. Optimal conditions for the reaction were determined as follows: 45 minutes, 20řC in dark. The method of purification of sample from undesired substances was not successful. However HPLC conditions were fully optimized, the column-switching technique was not effective enough. Chromatograms were not so selective, and desired level of sensitivity was not reached.
Detailed record
Analysis of atorvastatine by HPLC with using of fluorescence derivatization
 Líška, Marián ; Šatínský, Dalibor (advisor) ; Solich, Petr (referee)
10.2 Determination of atorvastatin by high performance liquid chromatography with fluorescence detection The main aim of this work was to develop a method for determination of atorvastatin by HPLC, which would be sensitive enough to determine plasmatic concentration of atorvastatin (ng/ml). UV-VIS detection is not sensitive enough for this purpose, therefore fluorescence detection was used. Atorvastatin was derived by 4-brommethyl-6,7-dimethoxycoumarin in N,N- dimethylformamide. Potassium carbonate and 18-crown-6-ether were used as catalysts. Optimal conditions for the reaction were determined as follows: 45 minutes, 20řC in dark. The method of purification of sample from undesired substances was not successful. However HPLC conditions were fully optimized, the column-switching technique was not effective enough. Chromatograms were not so selective, and desired level of sensitivity was not reached.
Detailed record

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