National Repository of Grey Literature 30 records found  beginprevious13 - 22next  jump to record: Search took 0.00 seconds. 
Immunogenicity of stem cells and their derivatives
Doležalová, Nikola ; Holáň, Vladimír (advisor) ; Kubinová, Šárka (referee)
Immunogenicity of stem cells and their derivatives Nikola Doležalová Abstract Stem cells (SCs) have the potential to be used in regenerative medicine on the basis of their differentiation capacity and promising immunological properties, including low expression of histocompatibility antigens and costimulatory molecules, or secretion of suppressive cytokines. Their immunogenicity has often been ignored in the past but it is becoming clear that rejection of genetically incompatible SCs represents a very common issue. At present, SCs are extensively studied from the immunological point of view, since it represents a critical aspect of the safety of SC therapy. This thesis presents an overview of current knowledge about immunogenicity of SCs and their derivatives, including both pluripotent SCs (embryonic and induced pluripotent SCs) and adult SCs (mesenchymal, limbal, neural, haematopoietic and umbilical cord blood SCs). The expression of immunologically relevant molecules on their surface and interaction with the immune cells in vitro and in vivo will be discussed, together with suggestions for overcoming the immunological barriers for transplantation. Detailed analysis of these aspects necessarily has to precede the safe clinical translation of SC therapies.
Treatment of spinal cord injury by transplantation different types of stem cells
Dubišová, Jana ; Kubinová, Šárka (advisor) ; Cizkova, Dasa (referee)
Spinal cord injury (SCI) is complicated injury with serious socioeconomic consequences for the patient and his whole family. Big difficulty cause also extremely high living expenses for the patient with this type of injury. That's why there is a need for therapeutic methods which would help patients after SCI to recover the lost functions and be able at least partially to return to their normal life. Different therapeutic methods are being used for SCI treatment. In this study we used four various types of stem cells: human bone marrow stem cells (hBM-MSCs), human umbilical cord mesenchymal stem cells (hUC-MSCs), neural precursors derived from induced pluripotent stem cells (iPS-NPs) and neural stem cell line derived from human fetal spinal cord tissue (SPC-01). These cells have been transplanted intrathecally or intraspinally 7 days after induction of the experimental model of SCI in the rat. We studied expressions of genes related to neurogenesis, growth factors and inflammation 10 and 28 days after SCI. Our analysis showed significant changes in gene expression 10 days after SCI. Significant up-regulation in expression of vascular endothelial growth factor (Vegf), ciliary neurotrophic factor (Cntf) and interferon regulatory factor 5 (Irf5) were found after transplantation of hBM-MSCs and hUC-...
Variscan igneous activity at the Bohemicum/moldanubicum boundary
Kubínová, Šárka ; Holub, František (advisor) ; Pertoldová, Jaroslava (referee)
Variscan dike swarms associated with the Central Bohemian Plutonic Complex (CBPC) at the boundary between the Teplá-Barrandian and Moldanubian Units of the Bohemian Massif represent one of the most interesting geological phenomena. Frequency of dykes and their chemical variability do not have any comparable analogy in the whole European Variscides. This work is focused on the study of dyke rocks in the NE periphery of CBPC in geologically very complicated area with intrusions of predominantly deformed granitoids, contact metamorphosed sediments and magmatic rocks of "Islet Zone" with different protolith ages (forming remnants of the original roof of CBPC), deformed basic rocks of uncertain origin and age. The area extends up to the western boundary of the northernmost part of the Moldanubian high-grade metamorphic complex, the boundary itself being also tectonically problematic. Several localities with dyke rocks under study are situated in the area east of Senohraby (SE of Prague), on the northern side (right coast) of the Sázava river, and extend up to the area of Stříbrná Skalice. This area is rich in dykes of gabbro to diorite porphyry accompanied in some places with tonalite (rarely quartz diorite) porphyry and more rarely with amphibole lamprophyres (spessartite). Significantly younger dykes...
Mesenchymal stem cells for treatment of spinal cord injury
Dubišová, Jana ; Kubinová, Šárka (advisor) ; Hock, Miroslav (referee)
Poranenie miechy a jeho terapia pomocou mezenchymálnych kmeňových buniek Abstract Patients with serious spinal cord injury (SCI) have a little chance of a full return to their original life. This kind of injuries leaves lifelong consequences as on the patients themselves, as well as their surroundings and family. Considerable financial resources are, worldwide, granted for reintegratation of these patients into society. This work focuses on the description of spinal cord injury, its complexity and the possibility of treatment by new therapeutic methods of mesenchymal cells. The first section discusses the severity of spinal cord injury and its pathophysiology with the performance of subsequent clinical phases. The second part is dedicated to new treatment methods and approaches, with an emphasis on cell therapy. The third part presents treatment method using mesenchymal stem cells (MSCs), which immunomodulatory and anti-inflammatory properties, such as their ability to migration to the injured area and the possibility of genetic modification, made them a great candidates for the treatment of serious injuries. Keywords Spinal cord injury, stem cells, mesenchymal stem cells, cell therapy, regenerative medicine
Targeted differentiation of mesenchymal stem cells and their clinical application
Hámor, Peter ; Kubinová, Šárka (advisor) ; Trošan, Peter (referee)
The goal of this work is to point out possibilities of neuronal differentiation of mesenchymal stem cells and their application for clinical purposes, primary for repairing and regeneration of central nervous system tissues. Because this system works as a control center for functional features of the whole body, and treating this injuries and degenerations often bring many problems and obstacles, the possibility of using autologous cells for a transplantation or inducer of the natural regenerative properties of tissues is worth deeper research. This work progressively focuses on basic characteristics of stem cells and their differentiation potential, characterizing further mesenchymal stem cells together with possibilities of their isolation and cultivation. The main part of the text is formed by studies and methods used for targeted differentiation of mesenchymal stem cells and attempts of their transdifferentiation into neural cell line, together with present and possible future application of these cells in central nervous system therapies.
Completion of the area Údolní 53 for the Faculty of Architecture and Faculty of Fine Arts
Stejskal, Ivo ; Kubínová, Šárka (referee) ; Makovský, Zdeněk (advisor)
It is necessary to ask the question of the relationship and the corresponding forms of buildings of the faculty of architecture and faculty of fine arts with the public. Due to the efforts of the faculties to open up to the public, I find it undesirable to place new objects in the courtyard and hide behind the walls of historic buildings. Much more meaningful is the use of existing buildings at the boundary of the plot. It is important to protect our history, but the protection of existing buildings should not get to the point where it will defend the presence, be on the same level, and thus ensure its quality and together to shape the future. The newly allocated creative use of the original barracks has greater demands on space, whether capacity or its generosity. Therefore, it is necessary to customize the objects for the current function. The superstructure will help keep faculties in one building. An advantage is also the connection to existing networks or vertical communications. The proposed minimalist intervention is in contrast to the historic building, making it stand out, while the verticalism of the superstructure raises the object of the original barracks to the desired institution of the Faculty of Architecture and the Faculty of Fine Arts.
Problems of definition and classification of lamprophyres
Kubínová, Šárka ; Holub, František (advisor) ; Štemprok, Miroslav (referee)
Lamprophyres are mafit to ultramafic porphyritic rocks containing phenocrysts of hydrated mafic minerals, namely dark mica (phlogopite, biotite) and amphibole that are present also in the matrix and commonly are euhedral or nearly euhedral. On the other side, felsic minerals as various feldspars and feldspathoids, are present only in the matrix sometimes containing also significant amounts of primary carbonates. Lamprophyres are commonly divided into three major groups - (1) calc-alkaline, (2) alkaline, and (3) ultramafic. Some of them are closely related to kimberlites, lamproites and carbonatites and gradual transitions to these rocks as well as to other non-lamprophyric types do exist. Lamprophyres are volumetricaly minor group of igneous rocks.. In some respects, however, they are very important, particularly for our understanding of complicated petrogenetic processes in the upper mantle and lower crust, and also diamond or gold prospecting. Significant role in proper lamprophyres classification is played by perception of processes of lamprophyres magmas forming and their possible interaction with crustal material or enrichment by some elements contained in mantle. Lamprophyres may be very similar to normal igneous rocks. These rocks can be distinguished only on the basis of some mineralogical...
Methodology of using low-temperature plasma to sterilize the wound
Dejneka, Alexandr ; Kubinová, Šárka ; Moravec, J. ; Bezděk, D.
The main aim of the methodology is sterilization of skin surface and wounds in veterinary medicine based on low-temperature plasma. Low-temperature plasma has a non-specific antimicrobial effect and therefore can be safely applied to the living tissue without causing any damage. The methodology describes a solution for sterilization of tissues and wounds with bacterial, fungal or yeast contamination. The method can also be used for antimicrobial decontamination as well as for healing of chronic and non-healing wounds. Methodology presents solutions sterilization of tissues and wounds with potential bacterial, fungal or yeast contamination. The method can also be used for antimicrobial decontamination and promote healing of chronic and non-healing wounds.
The Students themselves - Student Center Poříčí
Kubínová, Šárka ; Sirotek, Adam (referee) ; Makovský, Zdeněk (advisor)
The topic of my thesis is the student center at Poříčí - on the corner Táborského nábřeží and street Vídeňská. It proposes seven-storey building containing student housing, student`s canteen, sports facilities and a calm inner courtyard. The building is addressed clearly and simply. It closes to the busy Street Vídeňská and it opens towards the River.
Socio-cultural centre Dolní Kounice
Vašut, Štěpán ; Kubínová, Šárka (referee) ; Mikulášek, David (advisor)
My diploma thesis is based on the module Res Publica - Public works focusing on community buildings. Dozens of towns were surveyed, whether they are interested in reconstruction of their community or sociocultural centre, and eventually a small town Dolní Kounice was chosen especially because of the beauty of the surrounding countryside as well as the exciting native urbanism and architecture. This thesis deals with transformation of the cultural house from the 50s of the 20th century to a new cultural centre. The extension and inconvenient terrace were removed and the hall is supported by a new mass of the vestibule at the interface between interior and exterior, on the site of the former patio. Next, a new front of the building was introduced which increased the entrance of the house and created a space for a study room, small gym, meeting room and common rooms. The part of the house used for housing units is designed only with aesthetic details on the facade. The Culture Centre is divided into a set of materials in order to reduce the scale of the whole building. The entrance part mass, in contrast, is enhanced by its division and the chosen materials. In this way the originally embarrassing object of the Cultural Centre has been changed into a new centre with a clear architectural expression and the interior of the house is enriched by view of the beauty of the city.

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