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The methodology of in vitro cultuvation of opium poppy and production of homozygous materials for breeding
Klíma, Miroslav ; Vyvadilová, Miroslava ; Kučera, Vratislav
The methodology includes several options for preparing and maintaining initial donor F1 plants with respect to breeder’s possibilities. The next part presents the basic techniques for explant preparation, establishing of in vitro culture, regeneration of shoots and whole plants, transferring to non-sterile conditions, multiplying, identification and selection of diploid materials for further breeding. Attached there are specifications of cultivation media used. Important stages are documented with the help of figures.
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