National Repository of Grey Literature 379 records found  beginprevious212 - 221nextend  jump to record: Search took 0.00 seconds. 
Anthelmintic and other xenobiotic biotransformation in helminths and its contribution to resistance development
Prchal, Lukáš ; Szotáková, Barbora (advisor) ; Hrdý, Ivan (referee) ; Kučera, Radim (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate Mgr. Lukáš Prchal Supervisor prof. Ing. Barbora Szotáková, Ph.D. Title of Doctoral Thesis Anthelmintic and other xenobiotic biotransformation in helminths and its contribution to resistance development Parasitic helminths such as tapeworms, flukes or nematodes pose a threat for domestic, farm and wild living animals. Helminths cause significant health problems in animals. Moreover, they cause economical losses to farmers due to production decrease and treatment costs. The treatment with veterinary anthelmintics is still a basic method to fight off helminth infections. However, the long-term use of anthelmintics caused an emergence of resistance to anthelmintics. The increasing incidence of multiresistant strains of many helminth species is the greatest problem. Therefore, research of drug resistance mechanisms became an actual field of study. Increased biotransformation of anthelmintics, transport out of parasite bodies and contact of lower developmental stages with anthelmintics in the environment are some of possible mechanisms that lead to decrease of anthelmintic therapy effectivity and resistance development. The presented thesis focuses on the study of these mechanisms in three helminth...
Development of a novel spectrophotometric method for zinc ions chelatation screening
Herbolt, Daniel ; Mladěnka, Přemysl (advisor) ; Kučera, Radim (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Daniel Herbolt, MSc. Supervisor: Assoc. Prof. Přemysl Mladěnka, Pharm.D., Ph.D. Title of rigorous thesis: Development of a novel spectrophotometric method for zinc ions chelatation screening Zinc is the only one trace metal, which is a cofactor for over 300 active enzymes. It is localized in all tissues and organs. Zinc deficiency can cause many diseases such as e.g. diabetes, disorders of hemostasis, and cancer. The toxicity of zinc is usually considered to be mild, but the imbalance of zinc, e.g. its presence in extracellular plaques by patients with Alzheimer's disease suggests a more complex relationship. The purpose of this work was to create a new spectrophotometric methodology for screening of chelation of zinc ions. The first step was to measure the spectra of the selected indicator dithizone and its complex with zinc and finding the ideal wavelengths for accurate determination of the linear concentration of zinc ions. Thereafter, the linearity was checked at two selected wavelengths. After that, the stability of dithizone and its complex with zinc was measured in relationship to time. The final phase was to validate the methodology on two known zinc chelators which were...
Stability Studies of Oral Liquid Preparations Using HPLC
Zahálka, Lukáš ; Solich, Petr (advisor) ; Kučera, Radim (referee) ; Muselík, Jan (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate Mgr. Lukáš Zahálka Supervisor Prof. RNDr. Petr Solich, CSc. Title of Doctoral Thesis Stability Studies of Oral Liquid Preparations Using HPLC According to the database of the Czech State Institute for Drug Control there are 59 thousand of registered drugs available in the Czech Republic, out of which 8 thousand drugs were marketed in July 2017; however, there are still some therapeutic needs that cannot be met by using of these commercially available drugs because of unsuitability of available dosage forms for being used, e.g., in pediatric patients. Thus, cooperation was established between the Faculty of Pharmacy in Hradec Králové (Department of Analytical Chemistry and Department of Pharmaceutical Technology) and the University Hospital in Motol (Prague) to address such therapeutic needs by developing of extemporaneous formulations of oral liquid preparations containing selected pharmaceutical active ingredients. Each project was composed of three major parts. First part was to develop several versions of drug formulations according to various requirements (e.g., sugar-free or preservative-free). This part was accomplished by the Department of Pharmaceutical Technology. The second (development...
The employment of HPLC in the field of chiral separations III.
Janetková, Monika ; Kučera, Radim (advisor) ; Nobilis, Milan (referee)
Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University Candidate: Monika Janetková Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Consultant: doc. Ing. Radim Vespalec, DrSc. Title of Thesis: The employment of HPLC in the field of chiral separations III. Boron cluster compounds are completely synthetic inorganic structures posessing unique properties. Exceptional characteristics allow them to prove competent among organic molecules predominantely used in medicine. Basic structure is made of boron and hydrogen atoms forming three-dimensional polyhedron with triangular planes. The atoms B-H-B are forming electrondeficient three-centred two-electron bond. Cloud of electrons that is shared inside polyhedron structure is the cause of delocalized negative charge of a whole molecule. Boron cluster compounds are likely to loose hydrogen proton in both, protic and aprotic solvents, ending in formation of an anion. Among their typical characteristics belong extreme hydrophobicity, metabolic and thermal stability. Considering stability and reactivity closo-, nido- and arachno-clusters with 11-12 vertices are largely used. Either of endo- or exoskeletal substitution might impair symmetry of a boron cluster molecule resulting in a formation of...
Utilisation of New Biomarkers for the Optimalization of Diagnostics and Therapy of Tumors of the Gastrointestinal Tract
Šafanda, Martin ; Kučera, Radek (advisor) ; Fínek, Jindřich (referee) ; Svobodová, Šárka (referee)
Utilisation of New Biomarkers for the Optimalization of Diagnostics and Therapy of Tumors of the Gastrointestinal Tract Introduction: Tumor markers are standard diagnostic tools. They are mainly used to monitor the course of the disease and to check the efficacy of the treatment. It is important to observe dynamics. Changing the level of the biomarker can prevent clinical manifestation and lead to early diagnosis of relapse, which in turn means improving the quality of life, including prolonging survival. Recently, we have encountered a number of diagnostic algorithms that suggest algorithms for estimating the risk of tumor presence or the risk of progression of cancer, using statistical methods. Objectives: The aim of this work is to verify new biomarkers for the diagnosis of gastric cancer and to develop an optimal algorithm for their use. Further, to evaluate the importance of cytokeratin markers - Tissue Polypeptide Antigen (TPA) and Tissue Polypeptide Specific Antigen (TPS) for the diagnosis of metastatic colorectal carcinoma in the liver. To carry out a pilot study of FGF23 levels in people with colorectal carcinoma and other gastrointestinal tumors. Methods and patients: Patient samples were analyzed using immunoradiometric, chemiluminescence and fluorescence assays. For each solved problem,...
The role of SDS in the events of the 1960s in Germany
Skotnicová, Anita ; Šmidrkal, Václav (advisor) ; Kučera, Rudolf (referee)
The 1960s were marked by a large number of demonstrations in the Western world, mostly attended by students. The increased civic activity of the young generation first appeared in the US when students protested against the war in Vietnam. In the Federal Republic of Germany, the events in Vietnam also belonged to important topics, but at the same time long-term problems of the society escalated too. There have been changes at both political and cultural levels. In the second half of the 1960s, a series of stormy demonstrations took place in Germany over this constellation. This thesis focuses on the "Socialist German Student Union" (SDS), which became the imaginary engine of the student protest movement of the 1960s. At the beginning, the work presents a global context, then the main topics and milestones of the development in Germany in the political, social and cultural spheres. Secondly, the development of the SDS is outlined, with an emphasis on the main themes of the association. Then, the course of the demonstrations in the 1960s, SDS activity and the subsequent development after 1969 is analyzed. Finally the work offers an evaluation of SDS success in advancing its long-term goals in the 1960s.
Insulin like and other growth factors and tumors
Kučera, Radek ; Topolčan, Ondřej (advisor) ; Klimeš, Jiří (referee) ; Šafarčík, Kristian (referee)
Inzulinu podobné a jiné růstové faktory a nádory Insulin like and other growth factors and tumors Disertační práce Plzeň 2011 Mgr. Radek Kučera Summary The interaction between growth factors and cancer incidence and development has been discussed recently. First works suggesting possible connection between increased production of insulin like growth factors with an increased incidence of tumors came already from early 80s of the last century. The aim of my work was focused on evaluation of possible relation between insulin like growth factors or their binding proteins and tumors from different perspectives, to assess their significance and try to determine what role the IGFs and IGFBPs may play in the current tumor diagnostics. The work itself is divided into two parts, the theoretical and practical ones. In the theoretical part, I summarized the action of IGF in human body and also focused on previous findings on the role of IGF system in cancer diagnoses. In the practical part, divided into three subsections, I focused on investigation of relations in a large set of patients with different cancer diagnoses, in a group of women with breast cancer and, finally, I focused on changes of IGF1 levels during follow-up. Theoretical part Insulin-like growth factors (IGF) are peptides, that participate on growth...
Intersexuality in Germany. Scholarly ans Societal Debate around the Law Amendment in 2012-2013
Misařová, Adéla ; Kučera, Rudolf (advisor) ; Emler, David (referee)
Germany was the first country in Europe that took an important step to helping intersex people. Since the 1st of November 2013 the intersex new-born have been enacting an opportunity to withhold information about the gender in its personal documents. Germany is now among the few countries in the world that gave intersexed people the opportunity to stay for a lifetime, if they so desire, without a gender. Intersex people are classified as individuals who are born with ambiguous sex characteristics (such as genitalia, chromosomes and hormones). Therefore they can't fall into either of the male or the female sex category. Amendment to the german Civil Code, which allowed marking gender of intersex children in its personal documents with the letter "X", was preceded by intense debate in the field of professional, political and social. Partial discussions of this topic took place in Germany since the 90s and were carried out thanks to the interest groups of intersex people. The most important stimulus for the amendment to civil code was the opinion of the German Council for Ethics from February 2012. The loudest argument in discussions appeared, pointed in particular to the need to ban dangerous surgeries on intersex children, which caused them health problems in adulthood. Other reason for this...
Analytical and bioanalytical study of selected drugs using UHPLC
Klepalová, Libuše ; Kovaříková, Petra (advisor) ; Kučera, Radim (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of pharmaceutical chemistry and drug analysis Candidate: Libuše Klepalová Supervisor: doc. PharmDr. Petra Kovaříková, Ph.D. Title of the Master's Thesis: Analytical and bioanalytical study of selected drugs using UHPLC Abstract High-performance liquid chromatography coupled with mass spectrometry plays a crucial role in drug analysis from biological materials, mainly due to its sensitivity, specificity and ability to provide structural information on the analytes. Multiple myeloma is a serious hematologic disease, accounting for about 1 % of all oncological diseases. New drugs from the group of proteasome inhibitors namely, carfilzomib and bortezomib represent a great promises for patients with relapsing multiple myeloma or in the case of the resistant to other anticancer drugs. The aim of this work was to develop pilot chromatographic conditions for HPLC-MS analysis of carfilzomib and bortezomib and evaluate different extraction of these drugs from plasma. Separation was carried out on an Ascentis column C18 10 x 3 mm, 3 µm, (Supelco, Germany). Electrospray ionization with ion trap mass analyzer were used in both cases. The mobile phase consisting of water and acetonitrile (20:80) was the most appropriate for carfilzomib. The flow rate of...

National Repository of Grey Literature : 379 records found   beginprevious212 - 221nextend  jump to record:
See also: similar author names
1 KUČERA, Richard
7 Kučera, Radek
4 Kučera, Radim
4 Kučera, Roman
1 Kučera, Rostislav
3 Kučera, Rudolf
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