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The use of HPLC in the field of chiral separations IV.
Daněk, Alois ; Kučera, Radim (advisor) ; Štěrbová, Petra (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Alois Daněk Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Title of thesis: The employment of HPLC in the field od chiral separations IV. The requirements for the quality and safety of medicines are constantly growing at present. Drugs are often chiral substances. Therefore, the importance of chiral separations is increasing. Direct separations using chiral stationary phases are the most used in this field. Silica gel, which is most often used as a carrier for chiral selectors, may contain metal impurities on its surface, which may negatively affect the separation process. This thesis focuses on testing the influence of chromatographic conditions on chiral and achiral interactions of nine selected analytes with the stationary phase. A column with β-cyclodextrin as chiral selector was used for the testing.
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The role of Karel Schwarzenberg in the Czech-German relations
Duháčková, Klára ; Kunštát, Miroslav (advisor) ; Kučera, Rudolf (referee)
This bachelor thesis sums up the role of Czech politician Karel Schwarzenberg during the forming of the Czechoslovak politics as well as his impact on shaping the Czech and the global European policy. The first part of the thesis introduces the personality of Karel Schwarzenberg followed by a brief introduction of the house of Schwarzenberg and the influence the Czech aristocracy had on the land during the 1930s. The second part of the thesis is focused on the Schwarzenberg's exile in Vienna, primarily on his role as a chairman in the International Helsinki Federation and the beginning of his cooperation with Václav Havel. The following part depicts his return to Prague, which was associated with the serving as the chancellor of the presidential office and the ongoing negotiations regarding the Czechoslovak- German Treaty on Good Neighbourliness and Friendly Cooperation. In 1992 Schwarzenberg left Prague Castle and primarily devoted to his foundational, cultural and publishing activities. The main emphasis is put on his role of the Minister of Foreign Affairs of the Czech Republic, whom he was between the years 2007 and 2009 and then again during the years 2010 and 2013. The thesis demonstrates the Czech-German mutual relationship within the purview of Karel Schwarzenberg.
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The Fight for the Freedom of Speech: Baden and German Liberalism in the 1830s
Návojová, Kateřina ; Kučera, Rudolf (advisor) ; Emler, David (referee)
This master thesis examines the political and particular media situation in 1832, with the stress on spreading the liberal ideas and their attempt to influence the public via one typical German journal for this period. Over six months, there was an extraordinary situation in Baden, when the censorship was restricted and suddenly there arose, for this period, unexpected possibilities for journalists. As a reaction to reduction of censorship, the journal Der Freisinnige was established. Although its existence was short-lived, manifestation of liberal values and reflection of contemporary opinions is evident. This journal was more important and powerful than any other because of the cooperation with one of the main initiators of liberalism, Karl von Rotteck, who was also active in politics. This connection of two roles was very powerful. Freedom of speech, understood as one of the major liberal demands, allowed the practical promotion of liberal ideas among citizens. This case study pays attention to this particular year, within the political inclinations and tendencies over the period 1815-1832 of the Grand Duchy of Baden and former German Confederation. There were clear connections to the events in the first third of the nineteen century, that made the short-time fall of censorship happen. At the...
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GC analysis of drugs with utilization of ionic liquid as a stationary phase I
Bärová, Karolína ; Kastner, Petr (advisor) ; Kučera, Radim (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Supervisor: PharmDr. Petr Kastner, Ph.D. Student: Karolína Bärová Title of Thesis: GC analysis of drugs with utilization of ionic liquid as a stationary phase I In this thesis, we focused on testing of three commercially obtainable columns with ionic liquids as stationary phases, which we tested on a model sample of ibuprofen and its four pharmacopoeial impurities after derivatization by alkylchloroformates. The results obtained on these columns were compared to results obtained on column with conventional nonpolar stationary phase. Furthermore, we tried to describe and explain retention mechanisms, which were important in these separations, we also tried to optimize methods for our individual columns and extract our derivatives into a nonpolar phase, with the view to diminishing the harmful effect of samples on columns. We appraise the results obtained on ionic liquid stationary phases and on a nonpolar stationary phase as quite comparable. The analysis of our derived samples can be performed on all tested ionic liquid columns and the analysis can be optimized for all of them. Apart from the change in selectivity on the most polar stationary phase, we did not notice any great...
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Nanofiltration
Heidrová, Hana ; Kučera, Radim (advisor) ; Holas, Ondřej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Nanofiltration Diploma thesis Candidate: Hana Heidrová Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Consultant: PharmDr. Tomáš Holas, Ph.D. Nanofiltration is a pressure-driven membrane process which is characterized by using semipermeable membranes with approximately 1 nm pores. This method is used abundantly for the separation of substances with low molecular weight. It is used for example in the process of product isolation in pharmaceutical industry. This diploma thesis is focused on the description of the behaviour of three commercially produced nanofiltration membranes and also on testing of their potential use in the production of ergot alkaloids. The theoretical part is focused especially on the description of pressure-driven membrane processes, the use of nanofiltration in various areas and also on current and historical use of ergot alkaloids in therapy and on the description of their properties. The practical part is oriented particularly on examination of effect of various experimental parameters on the retention of selected ergot alkaloids (primarily of lysergic and isolysergic acid). The results are compared and critically analysed. Keywords: pressure-driven...
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GC analysis of drugs using ionic liquid as stationary phase II
Hungerová, Lenka ; Kastner, Petr (advisor) ; Kučera, Radim (referee)
Charles university in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical chemistry and Pharmaceutical analysis Supervisor: PharmDr. Petr Kastner, Ph.D. Student: Lenka Hungerová Title of thesis: GC analysis of drugs using ionic liquid as a stationary phase II This thesis dealt with analysis of ibuprofen and its four pharmacopoeial impurities samples after derivatization by alkylchloroformates by gas chromatography (GC). The thesis builds on a thesis by Karolína Bärová (2018), who tested these samples on one column with a nonpolar stationary phase and on three columns with ionic liquid as a polar stationary phase. Three other columns with ionic liquids were used in this thesis, which showed higher polarity than the columns used by K. Bärová. There was an effort to describe the retention mechanisms, that were manifested here, and to optimize the conditions of the method on all the three columns. Following the previous experiments other options for improvement of the extraction yield of the derivatives into a nonpolar solvent were tested. The results indicate that all the three columns with ionic liquid as stationary phase can be used for analysis of these samples. The conditions of the method were optimized enough on all the columns. The extraction yield into the nonpolar...
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Factors which affect the purification and stability bioconjugates azaftalocyanines
Cichý, Jakub ; Kučera, Radim (advisor) ; Pilařová, Pavla (referee)
Rigorous thesis Factors which affect the purification and stability bioconjugates azaftalocyanines Mgr. Jakub Cichý Charles University, Faculty of Pharmacy in Hradci Králové, Department of Pharmaceutical chemistry and pharmaceutical analysis This work continues in diploma thesis Purification azaphthalocyanines bioconjugates using SPE. It deals with the stability of molecular probes (i.e. "mono" and "dual- labeled") after dilution and storage in a given time period (fridge/freezer). We focused on the content changes of free oligonucleotide chains and probes containing azaphthalocyanine dyes using HPLC with detection at 260 and 673 nm, at following time intervals 0 h, 14 h, 13 days and 33 days. We found that molecular AzaPc probes were stable without significant changes in their content and purity even after a month of storage. The biggest influence on the purity of the azaphthalocyanine bioconjugates had the evaporation metod of the mobile phase from the sample after the purification step. Furthermore, we studied the posibility of purification of the "dual-labeled" probes containing AzaPc by SPE. Unfortunatly, the result showed that the examined SPE cartridges were unable to separate successfully the "mono-labeled" the"dual-labeled" probes from each other. Nevertheless, the phenyl column DSC-Ph (500...
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Assessment of the suitability of the site for the location of the underground\nof SNF and RAW repository in terms of long-term safety - Horka site
Havlová, V. ; Pertoldová, J. ; Bukovská, Z. ; Hroch, T. ; Štědrá, V. ; Uhlík, J. ; Říha, J. ; Červinka, R. ; Baier, J. ; Buriánek, D. ; Černý, M. ; Dudková, I. ; Dušek, K. ; Franěk, J. ; Gondolli, J. ; Hokr, M. ; Holeček, J. ; Jankovec, J. ; Jelének, J. ; Jelínek, J. ; Kachlíková, R. ; Kaláb, Zdeněk ; Klajmon, M. ; Kolomá, K. ; Kouřil, M. ; Královcová, J. ; Kučera, R. ; Kunceová, E. ; Maryška, J. ; Milický, M. ; Nývlt, D. ; Ondra, P. ; Pacherová, P. ; Polák, M. ; Rapprich, V. ; Rukavičková, L. ; Steinová, J. ; Svoboda, J. ; Šír, P. ; Švagera, O. ; Vašíček, R. ; Vavro, Martin ; Vopálka, D. ; Zeman, J. ; Zeman, O.
This report summarises outline of a report for evaluation of the sites‘ suitability for the emplacement of a deep geological repository on the basis of the archive information and the geological survey conducted from surface.\nSites are evaluated according to the following criterion categories\n1. Geological characteristics of the site\n2. Hydrogeologic characteristics of the site\n3. Transport characteristics of the the site\n4. Site stability\n5. Factors, increasing probability of human intrusion into the site\n6. Acceptability of enginnered barrier properties under site conditions
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Assessment of the suitability of the site for the location of the SNF and RAW deep geological repository in terms of long-term safety - Dukovany - West site\n(EDU-Z).
Havlová, V. ; Pertoldová, J. ; Bukovská, Z. ; Hroch, T. ; Štědrá, V. ; Jankovec, J. ; Říha, J. ; Červinka, R. ; Černý, M. ; Dudková, A. ; Dušek, K. ; Franěk, J. ; Gondolli, J. ; Gvoždík, L. ; Hokr, M. ; Holeček, J. ; Hrdličková, K. ; Jelének, J. ; Jelínek, J. ; Kachlíková, R. ; Kaláb, Zdeněk ; Klajmon, M. ; Kolomá, K. ; Královcová, J. ; Kouřil, M. ; Kryštofová, E. ; Kučera, R. ; Kunceová, E. ; Maryška, J. ; Milický, M. ; Nývlt, D. ; Ondra, P. ; Pacherová, P. ; Polák, M. ; Rapprich, V. ; Rukavičková, L. ; Sedláčková, I. ; Vašíček, R. ; Vavro, Martin ; Vopálka, D. ; Uhlík, J. ; Zeman, J. ; Zeman, O.
This report summarises outline of a report for evaluation of the sites suitability for the emplacement of a deep geological repository on the basis of the archive information and the geological survey conducted from surface.\nSites are evaluated according to the following criterion categories\n1. Geological characteristics of the site\n2. Hydrogeologic characteristics of the site\n3. Transport characteristics of the the site\n4. Site stability\n5. Factors, increasing probability of human intrusion into the site\n6. Acceptability of enginnered barrier properties under site conditions
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Assessment of the suitability of the site for the location of the deep-water repository and RAW repository in terms of long-term safety - Čihadlo site
Havlová, V. ; Pertoldová, J. ; Bukovská, Z. ; Hroch, T. ; Štědrá, V. ; Polák, M. ; Říha, J. ; Červinka, R. ; Černý, M. ; Dudíková Schulmannová, B. ; Dudková, A. ; Dušek, K. ; Franěk, J. ; Gondolli, J. ; Gvoždík, L. ; Hokr, M. ; Holeček, J. ; Jankovec, J. ; Jelének, J. ; Jelínek, J. ; Kachlíková, R. ; Kaláb, Zdeněk ; Klajmon, M. ; Klomínský, J. ; Kolomá, K. ; Kouřil, M. ; Královcová, J. ; Kučera, R. ; Kunceová, E. ; Lednická, Markéta ; Maryška, J. ; Milický, M. ; Nývlt, D. ; Ondra, P. ; Rapprich, V. ; Rukavičková, L. ; Steinová, J. ; Svoboda, J. ; Šír, P. ; Švagera, O. ; Uhlík, J. ; Vašíček, R. ; Vavro, Martin ; Verner, K. ; Vopálka, D. ; Zeman, J. ; Zeman, O.
This report summarises outline of a report for evaluation of the sites suitability for the emplacement of a deep geological repository on the basis of the archive information and the geological survey conducted from surface.\nSites are evaluated according to the following criterion categories\n1. Geological characteristics of the site\n2. Hydrogeologic characteristics of the site\n3. Transport characteristics of the the site\n4. Site stability\n5. Factors, increasing probability of human intrusion into the site\n6. Acceptability of enginnered barrier properties under site conditions
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