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Senzory pro ptačí chřipku
Krejčová, Ludmila
Mutation rate of influenza viruses is up to a hundred times higher, when compared to other viruses. Thanks to the speed of mutational changes, influenza is considered as the strongest member of the group of potential pandemics agents. In order to prevent the occurrence and spread of pandemic, which could have impact on worldwide population, we need methods for rapid detection of each subtype of influenza viruses. One of the most progressive way of detection of biomolecules are electrochemical sensors and biosensors. The aim of the thesis was to design sensors and biosensors for influenza virus detection, especially avian influenza H5N1, that are based on the isolation of two different parts of influenza virion using paramagnetic particles, coupled with subsequent electrochemical detection of isolated target molecules. We designed and optimized methods for isolation and detection of influenza antigen (hemagglutinin) and influenza nucleic acid (DNA oligonucleotide derived from genomic RNA of influenza). In both cases, quantum dots (QDs) were used as the label of target molecules for electrochemical detection. Two fast, sensitive and low-cost method for isolation and electrochemical detection by square-wave voltammetry and differential pulse voltammetry were applied.
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