National Repository of Grey Literature 153 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
High-performance exploration and querying of selected multi-dimensional spaces in life sciences
Kratochvíl, Miroslav ; Bednárek, David (advisor) ; Glaab, Enrico (referee) ; Svozil, Daniel (referee)
This thesis studies, implements and experiments with specific application-oriented approaches for exploring and querying multi-dimensional datasets. The first part of the thesis scrutinizes indexing of the complex space of chemical compounds, and details a design of high-performance retrieval system for small molecules. The resulting system is then utilized within a wider context of federated search in heterogeneous data and metadata related to the chemical datasets. In the second part, the thesis focuses on fast visualization and exploration of many-dimensional data that originate from single- cell cytometry. Self-organizing maps are used to derive fast methods for analysis of the datasets, and used as a base for a novel data visualization algorithm. Finally, a similar approach is utilized for highly interactive exploration of multimedia datasets. The main contributions of the thesis comprise the advancement in optimization and methods for querying the chemical data implemented in the Sachem database cartridge, the federated, SPARQL-based interface to Sachem that provides the heterogeneous search support, dimensionality reduction algorithm EmbedSOM, design and implementation of the specific EmbedSOM-backed analysis tool for flow and mass cytometry, and design and implementation of the multimedia...
Heuristics for paths in maps
Kudláčková, Lada ; Mareš, Martin (advisor) ; Kratochvíl, Miroslav (referee)
The content of the thesis is a description of heuristic procedures, which are used to find the shortest paths in the graphs and verify their effec- tiveness on the actual data. It deals with heuristics for Dijkstra's algorithm, especially the A* algorithm, which uses a lower distance-to-target estimate. Heuristics are implemented and tested on the road network of the Czech Re- public. 1
The relation of music, trance and spirituality on the example of Czech psytrance scene - research on spiritual dimension in the perspective of musicians
Hvězdová, Zuzana ; Kratochvíl, Matěj (advisor) ; Kreisslová, Sandra (referee)
The genre of electronic dance music, psychedelic trance, is considered by its established scene as a meaningful source of personal spirituality. This thesis explores creator's perspective of this repetitive electronic dance music (typically DJs), thus complementing previous studies dealing primarily with the spiritual dimension in listener's perspective. Using own fieldwork and interviews, it describes specific socio-cultural conditions of Czech psytrance scene and traces the emic creator's interpretation in relations with music, trance and spirituality. Key words: DJ - electronic dance music - ethnomusicology - musical subculture - non- ordinary states of consciousness - psychedelic experience - psychedelic trance - psytrance - rave culture - spirituality - trance - transcendence
"Being vnye": Mylène Farmer and French Pop Music in Post-Soviet Russia
Kaznacheeva, Anna ; Havelková, Tereza (advisor) ; Kratochvíl, Matěj (referee)
The thesis examines how French popular music is experienced in post-Soviet Russia based on the Russian reception of the contemporary French singer Mylène Farmer. Farmer has remained popular in Russia ever since her songs and music videos entered the country following the collapse of the USSR. Producing complex and intellectually demanding audiovisual projects, the French artist attracts the audience aspiring to be perceived as today's Russian intellectual elite. The following analysis proves that while popular music is marginalised inside Russian cultural hierarchies, highbrow French songs tend to function as acknowledged sources of cultural capital. The thesis outlines the history of the Russian reception of French (popular) culture to show that Farmer's success in the country is closely connected with traditional Russian fascination with France. Drawing on the concept of the Imaginary West developed by the anthropologist Alexei Yurchak, the research follows how Russian attitude towards the West has shaped - and has been shaped by - the reception of French popular music. Key words: Mylène Farmer, popular music, videoclip, French pop, Russia, Imaginary West
Music as an Attribute of the Ethnic Identity of African Americans
Procházková, Eva ; Tyllner, Lubomír (advisor) ; Blüml, Jan (referee) ; Kratochvíl, Matěj (referee)
The objective of the dissertation is a detailed study of the interactions between music and ethnic identity of a selected ethnic group - African Americans during several historical periods, and the use of information obtained from literary sources as research starting point for fieldwork. The main research question is: Music and its role in the formation of an African American ethnic identity today. The introductory chapters of the dissertation give an overview of basic concepts and theories and briefly clarify the terms African Americans, African American music, and ethnic identity in terms of their interconnection. The main part of the theoretical part of the study focuses on the role of music in the process of an African American ethnic identity formation and is divided into five chapters, each of which discusses interactions between music and ethnic identity in different historical periods, for example during slavery times in North America and the Reconstruction of the South, during the Harlem Renaissance of the 1920s and The Civil Rights Movement of the 1950s and 1960s, or in the so-called post-soul era of the 1980s and 1990s. The summary of the existing knowledge from literary sources serves as a theoretical basis for a two-month qualitative fieldwork research conducted in Chicago, USA in...
Host-guest systems for efficient infrared solid-state emission
Rottenberg, Vojtěch ; Smolka, Rastislav (referee) ; Kratochvíl, Matouš (advisor)
The goal of this bachelor thesis is to compile a brief research on solid-phase fluorescence, to find suitable molecules for the preparation of host-guest systems that exhibit high quantum yields of solidphase fluorescence in the infrared region and to analyze them. These systems consist of a predominant molecule (host) that exhibits high quantum yields of fluorescence and a quantitatively less abundant molecule (guest) that exhibits fluorescence in the infrared part of the electromagnetic spectrum. The following molecules were chosen: (E)-N,N-diphenyl-4-(2-(5'-vinyl-[1,1':4',1''-terphenyl]-2'- yl)vinyl)aniline (–V) and (E)-5'-(4-(diphenylamino)styryl)-[1,1': 4',1''-terphenyl]-2'-carbonitrile (–CN) as a guest molecule and (E)-2-((5'-(4-(diphenylamino)styryl)-[1,1':4',1''-terphenyl]-2'- yl)methylene)malononitrile (–DCV) as a guest molecule. For sample analysis, emission and excitation spectra were measured using a fluorophore, quantum yields using an integrating sphere, and fluorescence lifetimes using the time-correlated single photon counting (TCSPC) method. From the spectral analyses and quantum yields, it was determined that the molecule (E)-5'-(4- (diphenylamino)styryl)-[1,1':4',1''-terphenyl]-2'-carbonitrile (–CN) is a suitable guest molecule, (E)-2- ((5'-(4-(diphenylamino)styryl)-[1,1': 4',1''-terphenyl]-2'-yl)methylene)malononitrile (–DCV) is not a suitable guest molecule and the suitability of using (E)-N,N-diphenyl-4-(2-(5'-vinyl-[1,1':4',1''- terphenyl]-2'-yl)vinyl)aniline (–V) as a guest molecule is inconclusive.
Resonance energy transfer in the study of polymer-vesicle interaction
Jízdná, Zuzana ; Kratochvíl, Matouš (referee) ; Mravec, Filip (advisor)
The presented bachelor's thesis dealt with the subject of preparation of cationic vesicular systems, formed by the CTAB and SDS surfactants, in order to study their mutual interaction with the hyaluronan polymer using Förster resonance energy transfer measurements. First, concentration series of vesicular systems with fluorophores were prepared. DPH, perylene and fluorescein were used as fluorophores. Afterwards, the fluorescence lifetime of selected fluorophores was measured by the TCSPC method. Then, the characteristics of the prepared vesicular systems were measured using the DLS and ESL methods. For the final measurement of the interaction between the vesicle and the polymer, a vesiculation solution was chosen based on all the measurements carried out, which contained DPH with a concentration of 3·10-5 mol/l and hyaluronan. Two molecular weights of hyaluronan (300 and 900 kDa) were tested at mass concentrations of 1, 5, 10, 50 and 100 mg/l. The measurement confirmed their mutual interaction, thus this system could find application in the medical sector.
Organic luminophores with long wavelenght emission
Kolaříková, Adéla ; Kratochvíl, Matouš (referee) ; Vala, Martin (advisor)
This bachelor thesis studies the possibility of achieving intense fluorescence in the red region of the spectrum using Förster resonance energy transfer (FRET) in nanoparticles. First, the optical properties of organic molecules that could be suitable for the creation of a so-called host-guest (HG) system consisting of an acceptor-donor pair were studied. The molecules studied were based on derivatives of diphenylstilbene containing the electron-donor group diphenylamine (DPA-DPS), which is linked to a differing electron-acceptor group via a -conjugated double bond system. The acceptor groups used, i.e., indandione (-IOO), vinyl ( V) and di(methoxycarbonyl)vinyl (-V(COOMe)2), differ from each other in structure and polarity, which was reflected by a change in the position of the fluorescence spectrum. In the HG systems studied, DPA-DPS-IOO always served as the guest (G1) and DPA-DPS-V and DPA-DPS-V(COOMe)2 always served as the host (H1 or H2). Nanoparticles from these substances (G1H1 and G1H2) were prepared by the nanoprecipitation method. FRET was observed for both of these systems. Upon excitation of the matrix, the energy was transferred to the guest G1, which subsequently fluoresced in the long-wavelength region. Furthermore, an increase in the quantum yield of the guest fluorescence was also observed for the nanoparticles formed from the G1H2 system, from 7% (powder) and 3.1% (nanoparticles) to 14% compared to both the powder form and nanoparticles formed from the guest alone. No increase in the quantum yield of guest fluorescence was observed for the nanoparticles of the G1H1 system. The results indicated that FRET can be an effective tool in developing nanoparticles exhibiting intense long-wavelength fluorescence for imaging.
Investigation of novel materials for organic solar cells
Varchenko, Marko ; Gavranović, Stevan (referee) ; Kratochvíl, Matouš (advisor)
Solar cells, organic semiconductors, donor materials, heterojunction, spin-coating.
Electrochemical evolution of hydrogen peroxide for biotechnological applications
Runštuková, Nikola ; Kratochvíl, Matouš (referee) ; Ehlich, Jiří (advisor)
This master‘s thesis deals with the connection of two distinct fields, electrochemistry and biotechnology, where electrochemically generated peroxide was subsequently used in bacterial cultivation. The peroxide was synthesized in an electrolytic cell by reducing oxygen at the cathode to which suitable electrical potential was applied. The aim of this thesis was to investigate the dependence of the amount of hydrogen peroxide produced on a few selected parameters, such as cathode potential, electrode material, the geometry of the measuring cell, or the stirring speed, and then to apply these findings in the continuous stressing of the bacterium Cupriavidus necator H16, in order to increase the production of polyhydroxyalkanoates. The selected parameters were first tested in small-scale measuring cells and then experiments were carried out on a larger scale, in Erlenmeyer flasks. During the experiments, the amount of electric current produced was measured and also the peroxide concentration was examined spectrophotometrically along with the oxygen concentration measured by using an optical sensor. Based on the obtained results, bacteria were cultivated in flasks with a built-in system of steel electrodes, due to which oxidative stress in the form of in situ generated hydrogen peroxide was continuously applied. The bacterial cultures were evaluated by measuring the change in optical density, gravimetric analysis and GC-FID analysis. It was found that even small amounts of hydrogen peroxide (~25 M) can induce a stress response in the organism in the form of increased polyhydroxybutyrate (PHB) synthesis. A percentage increase in the PHB content of the biomass was achieved, however, the overall polymer yield was lower due to lower biomass growth.

National Repository of Grey Literature : 153 records found   1 - 10nextend  jump to record:
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