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Experimental Treatment of the Spinal Cord Injury and Clinical Treatment of Thoraco-Lumbar Vertebral Fractures
Krůpa, Petr ; Řehák, Svatopluk (advisor) ; Hejčl, Aleš (referee) ; Hrabálek, Lumír (referee)
Vertebral body fractures together with the spinal cord injury (SCI) remain a challenging medical condition. While management of the thoracic-lumbar (Th-L) vertebral fractures belongs to basic neurosurgical procedures with a usually excellent outcome, treatment of the severe spinal cord injury has mostly unsatisfactory results and thus novel approaches are highly in demand. In this study, experimental treatment of the SCI in a rat model using human mesenchymal stem cells derived from Wharton's jelly (WJ-MSCs) and a novel highly water soluble, nano-formulated curcumin is evaluated. Furthermore, a novel method of mini- invasive percutaneous posterior stabilisation (MIS) of Th-L vertebral fractures is retrospectively compared with classical open posterior (OPEN) procedure. To assess the effectivity of hWJ-MSCs treatment in the ischemic-compression model of SCI in rats, different dosages (0.5 or 1.5 million cells) and repeated applications were compared. Cells or saline were applied intrathecally by lumbar puncture once, or in three consecutive weeks after injury. Nanocurcumin and a vehicle nanocarrier as a control were delivered both locally, immediately after the spinal cord injury, and subcutaneously during the four consecutive weeks after SCI. Rats were assessed for locomotor skills (BBB, flat beam,...
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