|
|
Spirulina platensis effects on endothelial expression of P-selektin in mice
Králová, Monika ; Nachtigal, Petr (advisor) ; Mičuda, Stanislav (referee)
Monika Králová Spirulina platensis effects on endothelial expression of P-selectin in mice. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Background: We studied endothelial expression of P-selectin in aorta of apoE-deficient mice after administration of Spirulina platensis. Expression of P-selectin was quantified by using stereological methods. Methods: We used C57BL/6J male mice with deficiency of apolipoprotein E (apoE-/- ) in the experiment. Mice were divided into two groups. The control group was fed by atherogenic diet with 1% of cholesterol for 8 weeks. Mice from the Spirulina platensis group were fed by the same atherogenic diet with the addition of 40 mg Spirulina platensis. We performed a biochemical analysis of blood. Immunohistochemical analysis was performed in a 1 cm aortic sinus and the aortic arch. A series of cross sections with a thickness of 7 µm were cut for the immunohistochemistry and stereology. Detection of expression of P-selectin was performed by avidin-biotic method (ABC) with visualization by diaminobenzidin (DAB). Results: Biochemical analysis did not show significant changes in total cholesterol in mice treated with Spirulina in comparsion with control mice. Expression of P-selectin was observed in all mice. There were no...
|
|
|
Role of teaching assistant at primary school with Montessori program
Králová, Monika ; Bartoňová, Miroslava (advisor) ; Hádková, Kateřina (referee)
This bachelor's thesis deals with the topic of the role of teacher assistant in a primary school classroom with Montessori program, focusing on the inclusion of pupils with special educational needs and on the position of a teacher assistant in Montessori learning environment. We defined the education of pupils with special educational needs, including the most frequent diagnoses we encounter during the research. The whole paper is grounded in the legislation of the Czech Republic in the field of primary education. The paper also describes alternative education and its most common varieties and includes the characteristics of the education following Maria Montessori philosophy. This part is dedicated to the principles of the Montessori method, to the role of a Montessori teacher, to the characteristics of the classroom environment as well as to the teaching organisation, including teaching forms and methods. The object of this research was to find out about the current situation in educating pupils with special educational needs and in their support in the alternative classroom environment. Another object was to describe the work of a teacher assistant in a Montessori school, defining the basic differences between their role in a Montessori school and in a traditional school. The practical part of...
|
|
|
Hierarchy formation in the cosplay community
Králová, Monika ; Soukup, Martin (advisor) ; Zezulková, Markéta (referee)
The popularity of wearing costumes and masks has a long tradition. The phenomenon called cosplay began to be discussed for the first time in the 1980s. Cosplayers are fans of media content that not only wear costumes, but they also try to imitate the character's character on festivals. Some individuals, thanks to their activity, become celebrities themselves within the community. Fans may not always be just a passive group of recipients of media content. This thesis focuses on how these positions can be achieved and how the hierarchy is shaped in the Czech cosplay community. The theoretical framework is based on findings of fan studies. Methods used for data collection include participant observation and semi-structured interviews. The timeframe for data collection is the period from May 2018 to October 2018. For analysis were used the grounded theory principles, namely open and axial coding. The output is a paradigmatic model. This research has brought new information related to the topic of hierarchy in fan communities. Not only has the high activity of the individuals but also the social ties had an influence on hierarchy formation. This work also reflected the Czech cosplay community.
|
|
| |
|
|
Analysis of Czech films attendance in 2010-2015
Králová, Monika ; DANIELIS, Aleš (advisor) ; Badač, Peter (referee)
The cinema still belongs to the main place for distribution of the audiovisual content, despite other growing possibilities. The cinema attendance had various tendencies in the past and it even increased last year. However, one thing is the general attendance and the other one is only the attendance of the inland production - and exactly this issue is the topic of this thesis.
The first part of this thesis is primarily focused on the results and the statistics regarding the attendance of the Czech films. Specifically, it is about the results of the attendance and the revenues, differences between classic cinemas and multiplexes. One chapter is also concerned with the genres of Czech films. The following part describes particular factors which can influence the cinema attendance and these factors are considering the results in the first part. The final part presents the summary of this analysis.
|
|
|
Spirulina platensis effects on endothelial expression of P-selektin in mice
Králová, Monika ; Nachtigal, Petr (advisor) ; Mičuda, Stanislav (referee)
Monika Králová Spirulina platensis effects on endothelial expression of P-selectin in mice. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Background: We studied endothelial expression of P-selectin in aorta of apoE-deficient mice after administration of Spirulina platensis. Expression of P-selectin was quantified by using stereological methods. Methods: We used C57BL/6J male mice with deficiency of apolipoprotein E (apoE-/- ) in the experiment. Mice were divided into two groups. The control group was fed by atherogenic diet with 1% of cholesterol for 8 weeks. Mice from the Spirulina platensis group were fed by the same atherogenic diet with the addition of 40 mg Spirulina platensis. We performed a biochemical analysis of blood. Immunohistochemical analysis was performed in a 1 cm aortic sinus and the aortic arch. A series of cross sections with a thickness of 7 µm were cut for the immunohistochemistry and stereology. Detection of expression of P-selectin was performed by avidin-biotic method (ABC) with visualization by diaminobenzidin (DAB). Results: Biochemical analysis did not show significant changes in total cholesterol in mice treated with Spirulina in comparsion with control mice. Expression of P-selectin was observed in all mice. There were no...
|
guest ::
RSS feed.