National Repository of Grey Literature 108 records found  previous11 - 20nextend  jump to record: Search took 0.00 seconds. 
Genetic factors affecting course of selected forms of nephrotic syndrome
Šafaříková, Markéta ; Štekrová, Jitka (advisor) ; Král, Jiří (referee)
Nephrotic syndrome (NS) is characterized by proteinuria, hypalbuminemia and edemas. It occurs during first and second glomerulopathies. This disease can be divided into two groups: primary (idiopathic) and secondary. The heredity of the familial nephrotic syndrome is autosomal dominant and autosomal recessive. There are four most important genes that condition the formation of hereditary nephrotic syndrome in adult patienst. These genes are ACTN4, CD2AP, NPHS2 and TRPC6. The gene ACTN4, which encodes protein α-actinin 4, is responsible for the autosomal dominant form of focal segmental glomerulosclerosis (FSGS). FSGS is included in first glomerulopathies. α-Actinin 4 was also researched for some types of carcinomas. There was performed the mutational analysis of the gene ACTN4 on the set of 48 patients with nephrotic syndrome in this diploma thesis. High resolution melting (HRM) analysis and sequencing selected samples were used during this mutation detection. During this process many published and unpublished SNPs and one unpublished candidate mutation that could have causal associations with FSGS were found.
Evolution of the karyotype in two spider families, Atypidae and Pholcidae
Pastuchová, Markéta ; Král, Jiří (advisor) ; Kočárek, Eduard (referee)
Evolution of the karyotype in two spider families, Atypidae and Pholcidae (Araneae) Summary From cytogenetic point of view spiders are very diversified group, they exhibit great diversity in diploid chromosome number from 7 Ariadna lateralis (Segestriidae) up to 128 Cyclocosmia siamensis (Ctenizidae). Till recently karyotypes of almost all spiders were supposed to consist exclusively of acrocentric chromosomes including X1X20 sex chromosome system. However, this idea is based mostly on research of an advanced clade - entelegyne lineage of araneomorph spiders. Karyotypes of mygalomorph and haplogyne spiders remain nearly unexplored. Presented study is focused on cytogenetics of eight representatives of the family Pholcidae (Araneomorphae, Haplogyne) and Atypus piceus and A. affinis (Mygalomorphae, fam.Atypidae). Karyotypes, course of meiosis and distribution of nucleolar organizer regions (NOR) have been investigated in selected pholcid species and in one representative of the family Sicariidae (Araneomorphae, Haplogyne). Chromosomes of these groups are mostly biarmed (metacentric or submetacentric) and sex chromosome systems are diversified. Furthermore, distribution of constitutive heterochromatin and NOR has been analysed in A. piceus (2n0' = 41,XO) and A. affinis (2n0' = 14,XY). Key words: Atypidae,...
Giardia intestinalis karyotypes
Hudosová, Lenka ; Nohýnková, Eva (advisor) ; Král, Jiří (referee)
Giardia intestinalis is a parasitic protist that causes one of the most common diarrheal disease of parasite origin. The cell of Giardia contains two nuclei with unknown number of chromosomes until recently. Karyotype was determined five years ago using conventional cytogenetic method by Tůmová and collaborators. In my work, I assessed karyotype of four isolates, six lines and three clonal lines by the same method. It was confirmed, that two nuclei within one cell could differ in chromosome number, the differences found were 1, 2 or 6 chromosomes. Aneuploid number of chromosomes was found too. In case that both nuclei within single cell contained the same number of chromosomes, there were 10 chromosomes indentified in each nucleus. It was also revealed, that karyotype is not specific feature for different genetic groups (in this work assemblages A and E). Karyotype can be different even among lines and clonal populations derived from the same isolate. Changes in karyotype in the course of in vitro cultivation were detected within three populations. Results are discussed in relation to known facts.
Karyotype evolution of velvet spiders (Araneae: Eresidae)
Forman, Martin ; Král, Jiří (advisor) ; Rothová, Olga (referee)
Presented study is aimed to determine basic trends of karyotype evolution in velvet spiders (Eresidae; Araneae). Eresids are burrowing spiders; they includes also some social species. Karyotypes of 16 species of the family Eresidae as well as 2 species of the other families of the superfamily Eresoidea, Hersiliidae and Oecobiidae, are presented. Furthermore, DNA content and base proportion was determined in 14 species. In two species, DNA content of sex chromosomes was also measured. Obtained results revealed considerable variability of diploid numbers, and sex chromosomes systems in eresids. Obtained karyotype data allow to divide eresids into four groups. Karyotypes of the genera Gandanameno, Dresserus and S. lineatus are close to proposed ancestral karyotype of entelegynae spiders and they show the ancestral state of karyotype in velvet spiders. Karyotypes of S. lineatus and E. annulipes differ substantially from other representatives of the genera Stegodyphus and Eresus which indicates paraphyly of these genera. Karyotypes of basal forms are formed by acrocentric chromosomes. Further evolution of eresid karyotypes included considerable reduction of diploid numbers as well as changes of chromosome morphology. Social species of the genus Stegodyphus show tendency to reduce diploid numbers by...
Molekulárna charakterizácia vybraných obranných faktorov v čeľadi Lumbricidae
Mančíková, Veronika ; Bilej, Martin (advisor) ; Král, Jiří (referee)
Earthworms belonging to oligochaete annelids have been a model for comparative immunology for over 40 years. They possess various defense mechanisms efficiently recognizing and responding to non-self substances. Among these there are molecules with many biological activities including cytolytic, antimicrobial and proteolytic. This work is aimed to compare the immunological features of two closely related earthworm species Eisenia andrei and Eisenia fetida. Due to many morphological and life cycle similarities they have been, until recently, regarded as members of subspecies. Interestingly, their natural habitat varies considerably, and it was of particular interest to investigate how these environmental differences affect the features of innate immunity of both species. Key words: annelids, innate immunity, Eisenia andrei, Eisenia fetida, CCF, fetidin, lysenin, lysozyme
The role of cohesin genes in the meiosis of male house mouse
Šebestová, Lenka ; Trachtulec, Zdeněk (advisor) ; Král, Jiří (referee)
Cohesin genes play an important role in cell division. They ensure proper chromosome segregation during mitosis and meiosis. This study is focused on the role of cohesin genes during meiosis in male house mouse (Mus musculus). At first, this study introduces key processes of mammalian meiosis. Next, the structure of cohesin complex is described; it consists of a heterodimer SMC proteins - SMC3 and SMC1α or SMC1β, which are enclosed to the ring by cleavable subunit RAD21, RAD21L or REC8. Fourth subunit - a STAG protein (STAG1, STAG2 or STAG3) associates with the cleavable subunit. Meiotic function of specific cohesin proteins (SMC1β, RAD21L, REC8 and STAG3) as deduced from the phenotypes of the deficiencies of their genes in male mouse is depicted. All these four genes are necessary for many processes during meiosis, - e.q. sister chromatid cohesion maintenance, synapsis and recombination. STAG3, SMC1β, and REC8 are necessary for centromeric cohesion. STAG3 and RAD21L are important for the assembly of the remaining cohesin subunits. The most important phenotype of deficiency of all four genes is the complete meiotic arrest in male prophase I. Therefore, cohesin research is important for the investigation of the causes of sterility in mammals. key words: cohesin, meiosis, spermatogenesis, mouse,...

National Repository of Grey Literature : 108 records found   previous11 - 20nextend  jump to record:
See also: similar author names
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13 Král, Jakub
37 Král, Jan
1 Král, Jan (Ing.)
1 Král, Jan (RNDr.)
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2 Král, Jindřich
4 Král, Josef
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