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Some aggressive rock environment in the area of Prague, the groundwater and the associated protection of building structures
Štěpán, Jiří ; Král, Jan (advisor) ; Chaloupka, David (referee)
This master thesis deals with groundwater aggressiveness on one sheet of detailed engineering geology map of Prague 1:5000, sheet Praha 2-3. The aim of this thesis si an effort to verify how much geological environment participates in chemical composition of groundwater. The chemical analyses were evaluated using limit values stated in valid norm ČSN EN 206 Concrete - Specification, performance, production and conformity. The chemical analyses that analysed aggressive components (SO4 2- , CO2, pH, NH4 + , Mg2+ ) and total dissolved solids discovered that the geological environment affects the chemical composition of groundwater very strongly. The chemical composition of groundwater is influenced by groundwater velocity and therefore by the time which the water stays in contact with the geological environment, by local composition of the rock and its accessories especially pyrite. Groundwater in Ordovician formations in the area of interest is usually contains high amount of dissolved solids and it is calcium-sulphate. Groundwater of Dobrotiv and Libeň formations are significantly less aggressive than groundwater of Letná, Vinice and Zahořany formation. The chemical composition of groundwater in Peruc-Korycany formation corresponds with Letná formation on which it lies.
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Possibel approaches to gene therapy of cystic fibrosis
Král, Jan ; Bořek Dohalská, Lucie (advisor) ; Vaněk, Ondřej (referee)
1 Abstract Cystic fibrosis is an autosomal recesive disorder caused by a mutation in the CFTR gene, which leads to inefficiency or absence of CFTR chlorid channel. One way to induce production of CFTR protein in target cells, is to use gene therapy. The principle od gene therapy is to transfer DNA or mRNA molecules inside malfunctioning cells. The aim of this study was to optimise the detection of CFTR protein using the Western blot analysis. Then, using this method the effectiveness of CFTR-mRNA transfection was studied. To study the CFTR protein, a number of cell lines was used: a healthy human ephitelial cell line (NuLi-1), an ephitelial cell line with ΔF508 mutation (CuFi-1), and a human lung carcinoma cell line (A549). This study compared four different ways of cell lysis - lysis by sonication and lysis by three distinct lysis buffers. Lysis by RIPA buffer with protease inhibitors was determined for the detection of CFTR protein. Moreover, three different primary monoclonal antibodies were also tested. The CF3 antibody, which is specific to an extracellular epitope of CFTR protein, was found able to detect CFTR protein specifically. A couple of different glycosylated forms of CFTR protein was detected. The highest amount of CFTR protein was determined in the NuLi-1 cell line. CFTR protein was also...
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Synthesis and biological evaluation of tacrine-amantadine derivatives
Král, Jan ; Opletalová, Veronika (advisor) ; Holas, Ondřej (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Student: Jan Král Supervisor: doc. RNDr. Veronika Opletalová, Ph.D. Consultant: PharmDr. Jan Korábečný, Ph.D. Title of diploma thesis: Synthesis and biological evaluation of tacrine-amantadine derivatives Alzheimer's disease (AD) is a fatal neurodegenerative disorder of brain. Nowadays there is only paliative treatment available, which can be further subdivided into two groups: acetylcholinesterase inhibitors (AChEIs) and N-methyl-D-aspartate (NMDA) receptor antagonist. Donepezil, rivastigmine and galantamine represents AChEIs currently available for AD treatment. Tacrine is the first AChEIs to be approved for AD treatment, however, it was withdrawn from the market due to its side effects, especially due to its hepatotoxicity. 7-Methoxytacrine (7-MEOTA) is less toxic tacrine derivative preserving pharmacological profile of tacrine. This diploma thesis describes synthesis of multifunctional 7-MEOTA- amantadine derivatives as potential drugs to confront AD. Using Ellmanʼs method, we have established their efficacy to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in vitro. All new synthesized hybrids from 7-MEOTA- amantadine family proved to be better...
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The Blanka tunnel, geological hazadrs and problems during boring
Sedláček, Václav ; Král, Jan (advisor) ; Lešner, Jeroným (referee)
The Blanka tunnel has two boring sections: Brusnice tunnel and Královská obora tunnel. Both tunnels were boring in difficult geologycal conditions, but the geology conditions of these tunnels are not same. Also they had not the same hazards during boring. There was used a spectrum of different technical works with hazard reduction purpose and safety process of boring. There were three serious accidents, wnich everyone made a creater on the surface, but they have not the same causes. This text has a task to describe a geological survey before and during the boring of both tunnels. How other reasons affected the process of boring? How the tunnels were bored? What events preceded the everyone of three major failure? This text does not deal with economical things of boring and effects of specific personal influence on the process of boring. Key words Tunnel, excavation, Blanka, Brusnice, Letná, Stromovka park, Vltava river, underground water, geology, ordovik, New Austrian Tunnelling method, pilot tunnel, calotte, bench, bottom, arche, bolt, lining, grouting, collapse of ceiling, failure, tube, shale, quarzite, soil, loess, ballast, clay
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Evaluation of Geotechnical Conditions in Vodochody Airport Area
Sotorník, Viktor ; Král, Jan (advisor) ; Polák, Pavel (referee)
This work looks into geotechnical conditions in Vodochody Airport area. In this work research into available literature, sampling and laboratory processing of the samples were conducted. Based on the results of laboratory examinations, evaluation of suitability of the tested materials from the viewpoint of corresponding standards was accomplished. Three geological cross sections were also constructed. Basic classification analysis and additional tests after ČSN 73 6133 (2010), Chapter 4 were conducted. Both archival documentation and tests results prove significant variability of geological setting in the area of interest. The work describes current unsatisfactory state of the airport's runway and proposes one alternative of reconstruction. Foundation conditions of further constructions are also considered. Lastly the work points out several problems which may occur in the area and presents findings that will need to pay attention to in case of further exploration.
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Secondary compression of Brno Tegel
Pavlová, Martina ; Boháč, Jan (advisor) ; Král, Jan (referee) ; Rozsypal, Alexandr (referee)
Observation and measurement of long-term volumetric deformation of clay are important in tackling different geotechnical and geological issues. The area of interest is the northern part of Vienna Basin, where Miocene clays sedimented. It is locally known as Brno Tegel. The Thesis deals with the secondary compression of clays, which can be defined by the Cα/Cc concept. The one-dimensional creep can be determined using the numerical values of the coefficient of secondary compression Cα and subsequently the thickness of overburden of sediments can be estimated. The two different theories, known as Hypothesis A and B are also discussed, which have been used to interpret the effect of creep during the primary consolidation phase. The laboratory measurements of long-term oedometric tests are presented for two undisturbed samples and one reconstituted sample of Brno clay. A nonstandard behaviour in uniaxial creep is also discussed, namely the observed diffusion collapse. Powered by TCPDF (www.tcpdf.org)
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Groundwater aggression and the impact of geological environment
Štěpán, Jiří ; Král, Jan (advisor) ; Malát, Richard (referee)
This bachelor thesis deals with groundwater aggressiveness on two sheets of detailed engineering geology maps of Prague 1:5000. The aim of this thesis is an effort to verify how much geological environment of inner Prague participates in chemical composition of groundwater. The chemical analysis were evaluated using limit values stated in valid norm ČSN EN 206 Concrete - Specification, performance, production and conformity. The chemical analysis that analyzed aggressive components (SO4 2- , CO2 a pH) and total dissolved solids discovered that the geological environment affect the chemical composition of groundwater very strongly. Groundwater in Ordovician shale commonly contains high amount of dissolved solids with a lot of sulphate. In geological environment containing quartzite, greywacke, quartz and greywacke sandstone the groundwater has small amount of dissolved solids and no aggressive component is dominant as well as too high compared to the shale.
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Synthesis and biological evaluation of tacrine-amantadine derivatives
Král, Jan ; Opletalová, Veronika (advisor) ; Holas, Ondřej (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Student: Jan Král Supervisor: doc. RNDr. Veronika Opletalová, Ph.D. Consultant: PharmDr. Jan Korábečný, Ph.D. Title of diploma thesis: Synthesis and biological evaluation of tacrine-amantadine derivatives Alzheimer's disease (AD) is a fatal neurodegenerative disorder of brain. Nowadays there is only paliative treatment available, which can be further subdivided into two groups: acetylcholinesterase inhibitors (AChEIs) and N-methyl-D-aspartate (NMDA) receptor antagonist. Donepezil, rivastigmine and galantamine represents AChEIs currently available for AD treatment. Tacrine is the first AChEIs to be approved for AD treatment, however, it was withdrawn from the market due to its side effects, especially due to its hepatotoxicity. 7-Methoxytacrine (7-MEOTA) is less toxic tacrine derivative preserving pharmacological profile of tacrine. This diploma thesis describes synthesis of multifunctional 7-MEOTA- amantadine derivatives as potential drugs to confront AD. Using Ellmanʼs method, we have established their efficacy to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in vitro. All new synthesized hybrids from 7-MEOTA- amantadine family proved to be better...
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Evaluation of Geotechnical Conditions in Vodochody Airport Area
Sotorník, Viktor ; Král, Jan (advisor) ; Malát, Richard (referee)
This work looks into geotechnical conditions in Vodochody Airport area. In this work research into available literature, sampling and laboratory processing of the samples were conducted. Based on the results of laboratory examinations, evaluation of suitability of the tested materials from the viewpoint of corresponding standards was accomplished. Two geological cross sections were also constructed. Basic classification analysis and additional tests after ČSN 73 6133, Chapter 4 were conducted. Results show that after compaction the quaternary fluvial deposits which cover the examined area can be used in subbase of the airport road system. Other constructions, which do not require deep foundation methods, can too be founded on this soil with no complications. Lastly the work points out several problems which may occur in the area and presents findings that will need to pay attention to in case of further exploration.
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