National Repository of Grey Literature 155 records found  previous11 - 20nextend  jump to record: Search took 0.01 seconds. 
Introduction of an invertebrate animal model, Galleria Galleria mellonellamellonella, to study the in vivo efficacy of existing and newly synthesized, candidate antituberculotics
Kubíček, Jiří ; Konečná, Klára (advisor) ; Němečková, Ivana (referee)
Charles university, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Pharmacy Author: Jiří Kubíček Supervisor: RNDr. Klára Konečná, Ph.D. Title of thesis: Introduction of an invertebrate animal model, Galleria mellonella, to study the in vivo efficacy of existing and newly synthesized, candidate antituberculotics. Background: The aim of this diploma thesis has been to present an invertebrate organism, Galleria mellonella (G. mellonella), Pyralidae, as an alternative, more economically advantegous and morally more acceptable model for in vivo studies of an antituberculotics (anti-TB) efficacy. In the experimental part of this work, attention was paid to the optimization of methodological steps, that should lead to a higher reproducibility and validity of the obtained data. The effect of the disaggregating supplement, tyloxapol, on the growth of Mycobacterium aurum (M. aurum) was evaluated. The growth of this microbial strain in the culture medium, Middlebrook 7H9, was also mapped to determine the exponential growth phase. In the second part of the experiments, G. mellonella larvae were infected with a M. aurum bacterial strain, followed by an administration of the selected, commercially available anti-TB. Methods: To map the exponential phase of M....
Analysis of flagellar proteins in Clostridium difficile isolates of clinically relevant PCR-ribotypes
Houdková, Kateřina ; Konečná, Klára (advisor) ; Tichý, Aleš (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kateřina Houdková MPharm Supervisor: RNDr. Klára Konečná, Ph.D. Consultant: PharmDr. Jiří Dresler, Ph.D. Title of rigorous thesis: Analysis of flagellar proteins in C. difficile isolates of clinically relevant PCR-ribotypes Background: Strains of C. difficile of known human epidemiologic importance are associated with severe clinical features of C. difficile infection (CDI). In this study, a panel of eight different PCR-ribotypes (RTs) with their proteins released in vitro were subjected to analysis. The aim of this work is to monitor the relationship between secretions of individual proteins associated with flagellar formation and function in C. difficile strains of variable virulence. Methods: Within our research, a combination of tandem mass spectrometry with liquid chromatography was used. The semi-quantitative analysis employed label free quantification (LFQ) approach. Results: From the quantifiable proteins, 17 were significantly increased in functional annotations. Among them, several known factors connected with flagellar assembly and other functions were identified. Higher expression of selected flagellar proteins clearly distinguished RTs 027, 176, 005 and 012, confirming the pathogenic...
Evaluation of the effectiveness of selected methodical approaches for disagregation of staphylococcal biofilm biomass
Nosková, Martina ; Konečná, Klára (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Subject of study: Bioanalytical laboratory diagnostics in healtcare Student: Bc. Martina Nosková Supervisor: RNDr. Klára Konečná, Ph.D. Title: Evalution of the effectiveness of selected methodical approaches for disagregation of staphylococcal biofilm biomass Background: Evaluation of the impact of selected biochemical and physical approaches on the disaggregation of the biofilm community formed by the bacterium Staphylococcus aureus. For the biochemical approach was chosen enzyme proteinase K, the impact of sonication, shaking with/without glass beads was studied within the physical approaches. Methods: Staphylococcal biofilm was formed in vitro in two methodical configurations - in the wells of a microtiter plate and on pegs (analogy to the Calgary Biofilm Device). To evaluate the degree of consistency of the obtained data, which reflects the homogenity of the biofilm biomass disaggeragation, the "recovery" method, and the method of metabolic activity evaluation, using the Alamar Blue indicator. To express the degree of effectiveness of the chosen approaches on the overall disaggregation of the biofilm community, the method of quantification of biofilm biomass by crystal violet staining was...
Evaluation of antifungal activity of newly synthesized compounds
Bláhová, Lenka ; Voxová, Barbora (advisor) ; Konečná, Klára (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Lenka Bláhová Supervisor: PharmDr. Barbora Voxová Thesis title: Evaluation of antifungal activity of newly synthesized compounds English title: Evaluation of antifungal activity of newly synthesized compounds Thesis goal The goal of the thesis is to test new molecules as for their antifungal activity. Altogether 28 compounds of natural origin from the Department of Pharmaceutical Botany of the Faculty of Pharmacy of the Charles University were tested. Tested was the sensitivity to potentially antifungal substances with 8 micromycete strains, namely Candida albicans, Candida krusei, Candida parapsolosis, Candida tropicalis, Aspergillus fumigatus, Aspergillus flavus, Absidia corymbifera, Trichophyton interdigitale. Methods Molecules were tested with the broth microdilution method. Results 28 newly synthesized compounds were put to the test to prove the antimycotic effect. 11 out of them proved to be hopeful for further testing. Antifungal activity against all testing micromycetes was found with 5 compounds, 6 compounds proved antifungal activity against at least one micromycete. Conclusion The molecule structure of effective and ineffective compounds differs in the substitution of the benzene...
Evaluation of the effectiveness of selected methodical approaches for disagregation of staphylococcal biofilm biomass
Nosková, Martina ; Konečná, Klára (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Subject of study: Bioanalytical laboratory diagnostics in healtcare Student: Bc. Martina Nosková Supervisor: RNDr. Klára Konečná, Ph.D. Title: Evalution of the effectiveness of selected methodical approaches for disagregation of staphylococcal biofilm biomass Background: Evaluation of the impact of selected biochemical and physical approaches on the disaggregation of the biofilm community formed by the bacterium Staphylococcus aureus. For the biochemical approach was chosen enzyme proteinase K, the impact of sonication, shaking with/without glass beads was studied within the physical approaches. Methods: Staphylococcal biofilm was formed in vitro in two methodical configurations - in the wells of a microtiter plate and on pegs (analogy to the Calgary Biofilm Device). To evaluate the degree of consistency of the obtained data, which reflects the homogenity of the biofilm biomass disaggeragation, the "recovery" method, and the method of metabolic activity evaluation, using the Alamar Blue indicator. To express the degree of effectiveness of the chosen approaches on the overall disaggregation of the biofilm community, the method of quantification of biofilm biomass by crystal violet staining was...
Introduction of an invertebrate animal model, Galleria Galleria mellonellamellonella, to study the in vivo efficacy of existing and newly synthesized, candidate antituberculotics
Kubíček, Jiří ; Konečná, Klára (advisor) ; Němečková, Ivana (referee)
Charles university, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Pharmacy Author: Jiří Kubíček Supervisor: RNDr. Klára Konečná, Ph.D. Title of thesis: Introduction of an invertebrate animal model, Galleria mellonella, to study the in vivo efficacy of existing and newly synthesized, candidate antituberculotics. Background: The aim of this diploma thesis has been to present an invertebrate organism, Galleria mellonella (G. mellonella), Pyralidae, as an alternative, more economically advantegous and morally more acceptable model for in vivo studies of an antituberculotics (anti-TB) efficacy. In the experimental part of this work, attention was paid to the optimization of methodological steps, that should lead to a higher reproducibility and validity of the obtained data. The effect of the disaggregating supplement, tyloxapol, on the growth of Mycobacterium aurum (M. aurum) was evaluated. The growth of this microbial strain in the culture medium, Middlebrook 7H9, was also mapped to determine the exponential growth phase. In the second part of the experiments, G. mellonella larvae were infected with a M. aurum bacterial strain, followed by an administration of the selected, commercially available anti-TB. Methods: To map the exponential phase of M....
Novel compounds active against methicillin-resistant staphylococci and vancomycin-resistant enterococci
Pokorná, Anežka ; Krátký, Martin (advisor) ; Konečná, Klára (referee)
The bachelor thesis deals with compounds acting on methicillin-resistant staphylococci and vancomycin-resistant enterococci. It overviews suitable established or newly used antibiotics that are effective in combating these resistant strains, as well as new potentially useful compounds. The theoretical part includes information about particular strains, their characteristics, laboratory diagnostics and an overview of the diseases they cause, as well as information on mechanisms of resistance to clinically used antibiotics. Knowledge of these mechanisms is important for the future synthesis of new drugs. The second part reports the drugs themselves and promising compounds in development that are suitable for particular strains. The thesis discusses both already established compounds and potential compounds in various stages of (pre)clinical development. Keywords Antibiotics, biofilm, methicillin, methicillin-resistant staphylococci, natural derivatives, new compounds, resistance, vancomycin, vancomycin-resistant enterococci
Detection of antibodies reactive with F. tularensis in the early phase of infection.
Straková, Kristina ; Konečná, Klára (advisor) ; Janovská, Sylva (referee)
Kristina Straková Detection of antibodies reactive with F. tularensis in early phase of infection Thesis Charles University in Prague, Faculty Of Pharmacy in Hradec Králové Study program Pharmacy Because of its high virulence and mortality Francisella tularensis is considered to be a dangerous potential biological weapon and therefore this etiological agent is a suitable object of the primary research. Problems of an antibody response to the infection with intracellular pathogens are currently gaining in importance. Opinions and conclusions of various research teams differ, so this topic is up-to-date. The aim of this study was to find whether F. tularensis induces the antibody immune reaction in an early phase of infection (after 24 and 48 hours). The virulent strain of F. tularensis was used. We also intended to validate a feasibility of chosen methodology. Methods: We prepared a whole-cell bacteria lysate of F. tularensis holarctica FSC200. This lysate was separated by 2D electrophoresis with subsequent Western blotting. Proteins were marked by immunodetection using serum of BALB/c mice. Mice sera were collected 24 and 48 hours post challenge with F. tularensis. The serum of uninfected mice was utilized as control. Using comparative analysis, we tried to find proteins reacting only with the...
Production of secreted aspartic proteinases by pathogenic yeasts.
Šlajchová, Andrea ; Buchta, Vladimír (advisor) ; Konečná, Klára (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Andrea Šlajchová Supervisor: Doc. RNDr. Vladimír Buchta, CSc. Title of diploma thesis: Production of secreted aspartic proteinases by pathogenic yeasts The objective of this thesis was to identify the effect of farnesol as a quorum sensing molecule, which facilitates intracellular communication of Candida albicans, on the production of secreted aspartic proteinases (Sap). The theoretical section summarizes the morphology of Candida, the virulence factors, the illnessess cause by Candida and its treatment. A separate chapter focuses on secreted aspartic proteinases and on farnesol. The strains tested came from clinical isolates belonging to the Clinical Microbiology Institute of FN Hradec Kralove; two further strains were collected as well (10231 and 25188). The ATCC 10231 strain, which did not show farnesol production but the production of farnesol acid, was included in the test. Special media contained a source of bovine hemoglobin as the only source of nitrogen and had differing pHs. The strains were first pre-incubated to exogenous farnesol and later transfered to test media at various different concentrations of farnesol. The results were read after seven days of...

National Repository of Grey Literature : 155 records found   previous11 - 20nextend  jump to record:
See also: similar author names
1 Konečná, Kamila
2 Konečná, Karolina
2 Konečná, Karolína
13 Konečná, Kateřina
2 Konečná, Klaudie
5 Konečná, Kristýna
Interested in being notified about new results for this query?
Subscribe to the RSS feed.